LOCUS Exported 14856 bp ds-DNA circular SYN 15-JAN-2018
DEFINITION synthetic circular DNA
FEATURES Location/Qualifiers
source 1..14856
/organism="synthetic DNA construct"
/mol_type="other DNA"
CDS join(1..9,200..2017)
/codon_start=1
/product="beta-glucuronidase"
/label=GUS
/note="This version of the gusA gene has a 5' extension
with a catalase intron to ensure expression in plants but
not bacteria."
/translation="DLRNRRLVRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESAL
QESRAIAVPGSFNDQFADADIRNYAGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVW
VNNQEVMEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKK
QSYFHDFFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVSVEL
RDADQQVVATGQGTSGTLQVVNPHLWQPGEGYLYELEVTAKSQTESDIYPLRVGIRSVA
VKGQQFLINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYA
EEMLDWADEHGIVVIDETAAVGFQLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAIK
ELIARDKNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCDAH
TDTISDLFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVDTLA
GLHSMYTDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGNKKGI
FTRDRKPKSAAFLLQKRWTGMNFGEKPQQGGKQ"
intron 10..199
/label=cat1 intron
/note="castor bean catalase intron, modified"
CDS 2024..2041
/codon_start=1
/product="6xHis affinity tag"
/label=6xHis
/translation="HHHHHH"
terminator 2076..2328
/label=NOS terminator
/note="nopaline synthase terminator and poly(A) signal"
misc_feature 2350..2374
/label=RB T-DNA repeat
/note="right border repeat from nopaline C58 T-DNA"
CDS 3674..4303
/codon_start=1
/product="stability protein from the Pseudomonas plasmid
pVS1 (Heeb et al., 2000)"
/label=pVS1 StaA
/translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
misc_feature 6408..6548
/label=bom
/note="basis of mobility region from pBR322"
rep_origin complement(6734..7322)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(7409..8203)
/codon_start=1
/gene="aphA-3"
/product="aminoglycoside phosphotransferase"
/label=KanR
/note="confers resistance to kanamycin"
/translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
misc_feature 8628..8652
/label=LB T-DNA repeat
/note="left border repeat from nopaline C58 T-DNA"
polyA_signal 8730..8904
/label=CaMV poly(A) signal
/note="cauliflower mosaic virus polyadenylation signal"
CDS complement(8944..9969)
/codon_start=1
/gene="aph(4)-Ia"
/product="aminoglycoside phosphotransferase from E. coli"
/label=HygR
/note="confers resistance to hygromycin"
/translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
K"
promoter complement(10036..10713)
/label=CaMV 35S promoter (enhanced)
/note="cauliflower mosaic virus 35S promoter with a
duplicated enhancer region"
terminator 11036..11288
/label=NOS terminator
/note="nopaline synthase terminator and poly(A) signal"
misc_feature 11305..11546
/label=Stuffer
misc_feature 11553..12060
/label=Rice intron
promoter complement(12088..14080)
/label=Ubi promoter
/note="maize polyubiquitin gene promoter"
promoter 14489..14834
/label=CaMV 35S promoter
/note="strong constitutive promoter from cauliflower mosaic
virus"
ORIGIN
1 gatctgaggg taaatttcta gtttttctcc ttcattttct tggttaggac ccttttctct
61 ttttattttt ttgagctttg atctttcttt aaactgatct attttttaat tgattggtta
121 tggtgtaaat attacatagc tttaactgat aatctgatta ctttatttcg tgtgtctatg
181 atgatgatga tagttacaga accgacgact cgtccgtcct gtagaaaccc caacccgtga
241 aatcaaaaaa ctcgacggcc tgtgggcatt cagtctggat cgcgaaaact gtggaattga
301 tcagcgttgg tgggaaagcg cgttacaaga aagccgggca attgctgtgc caggcagttt
361 taacgatcag ttcgccgatg cagatattcg taattatgcg ggcaacgtct ggtatcagcg
421 cgaagtcttt ataccgaaag gttgggcagg ccagcgtatc gtgctgcgtt tcgatgcggt
481 cactcattac ggcaaagtgt gggtcaataa tcaggaagtg atggagcatc agggcggcta
541 tacgccattt gaagccgatg tcacgccgta tgttattgcc gggaaaagtg tacgtatcac
601 cgtttgtgtg aacaacgaac tgaactggca gactatcccg ccgggaatgg tgattaccga
661 cgaaaacggc aagaaaaagc agtcttactt ccatgatttc tttaactatg ccggaatcca
721 tcgcagcgta atgctctaca ccacgccgaa cacctgggtg gacgatatca ccgtggtgac
781 gcatgtcgcg caagactgta accacgcgtc tgttgactgg caggtggtgg ccaatggtga
841 tgtcagcgtt gaactgcgtg atgcggatca acaggtggtt gcaactggac aaggcactag
901 cgggactttg caagtggtga atccgcacct ctggcaaccg ggtgaaggtt atctctatga
961 actcgaagtc acagccaaaa gccagacaga gtctgatatc tacccgcttc gcgtcggcat
1021 ccggtcagtg gcagtgaagg gccaacagtt cctgattaac cacaaaccgt tctactttac
1081 tggctttggt cgtcatgaag atgcggactt acgtggcaaa ggattcgata acgtgctgat
1141 ggtgcacgac cacgcattaa tggactggat tggggccaac tcctaccgta cctcgcatta
1201 cccttacgct gaagagatgc tcgactgggc agatgaacat ggcatcgtgg tgattgatga
1261 aactgctgct gtcggctttc agctgtcttt aggcattggt ttcgaagcgg gcaacaagcc
1321 gaaagaactg tacagcgaag aggcagtcaa cggggaaact cagcaagcgc acttacaggc
1381 gattaaagag ctgatagcgc gtgacaaaaa ccacccaagc gtggtgatgt ggagtattgc
1441 caacgaaccg gatacccgtc cgcaaggtgc acgggaatat ttcgcgccac tggcggaagc
1501 aacgcgtaaa ctcgacccga cgcgtccgat cacctgcgtc aatgtaatgt tctgcgacgc
1561 tcacaccgat accatcagcg atctctttga tgtgctgtgc ctgaaccgtt attacggatg
1621 gtatgtccaa agcggcgatt tggaaacggc agagaaggta ctggaaaaag aacttctggc
1681 ctggcaggag aaactgcatc agccgattat catcaccgaa tacggcgtgg atacgttagc
1741 cgggctgcac tcaatgtaca ccgacatgtg gagtgaagag tatcagtgtg catggctgga
1801 tatgtatcac cgcgtctttg atcgcgtcag cgccgtcgtc ggtgaacagg tatggaattt
1861 cgccgatttt gcgacctcgc aaggcatatt gcgcgttggc ggtaacaaga aagggatctt
1921 cactcgcgac cgcaaaccga agtcggcggc ttttctgctg caaaaacgct ggactggcat
1981 gaacttcggt gaaaaaccgc agcagggagg caaacaagct agccaccacc accaccacca
2041 cgtgtgaatt acaggtgacc agctcgaatt tccccgatcg ttcaaacatt tggcaataaa
2101 gtttcttaag attgaatcct gttgccggtc ttgcgatgat tatcatataa tttctgttga
2161 attacgttaa gcatgtaata attaacatgt aatgcatgac gttatttatg agatgggttt
2221 ttatgattag agtcccgcaa ttatacattt aatacgcgat agaaaacaaa atatagcgcg
2281 caaactagga taaattatcg cgcgcggtgt catctatgtt actagatcgg gaattaaact
2341 atcagtgttt gacaggatat attggcgggt aaacctaaga gaaaagagcg tttattagaa
2401 taacggatat ttaaaagggc gtgaaaaggt ttatccgttc gtccatttgt atgtgcatgc
2461 caaccacagg gttcccctcg ggatcaaagt actttgatcc aacccctccg ctgctatagt
2521 gcagtcggct tctgacgttc agtgcagccg tcttctgaaa acgacatgtc gcacaagtcc
2581 taagttacgc gacaggctgc cgccctgccc ttttcctggc gttttcttgt cgcgtgtttt
2641 agtcgcataa agtagaatac ttgcgactag aaccggagac attacgccat gaacaagagc
2701 gccgccgctg gcctgctggg ctatgcccgc gtcagcaccg acgaccagga cttgaccaac
2761 caacgggccg aactgcacgc ggccggctgc accaagctgt tttccgagaa gatcaccggc
2821 accaggcgcg accgcccgga gctggccagg atgcttgacc acctacgccc tggcgacgtt
2881 gtgacagtga ccaggctaga ccgcctggcc cgcagcaccc gcgacctact ggacattgcc
2941 gagcgcatcc aggaggccgg cgcgggcctg cgtagcctgg cagagccgtg ggccgacacc
3001 accacgccgg ccggccgcat ggtgttgacc gtgttcgccg gcattgccga gttcgagcgt
3061 tccctaatca tcgaccgcac ccggagcggg cgcgaggccg ccaaggcccg aggcgtgaag
3121 tttggccccc gccctaccct caccccggca cagatcgcgc acgcccgcga gctgatcgac
3181 caggaaggcc gcaccgtgaa agaggcggct gcactgcttg gcgtgcatcg ctcgaccctg
3241 taccgcgcac ttgagcgcag cgaggaagtg acgcccaccg aggccaggcg gcgcggtgcc
3301 ttccgtgagg acgcattgac cgaggccgac gccctggcgg ccgccgagaa tgaacgccaa
3361 gaggaacaag catgaaaccg caccaggacg gccaggacga accgtttttc attaccgaag
3421 agatcgaggc ggagatgatc gcggccgggt acgtgttcga gccgcccgcg cacgtctcaa
3481 ccgtgcggct gcatgaaatc ctggccggtt tgtctgatgc caagctggcg gcctggccgg
3541 ccagcttggc cgctgaagaa accgagcgcc gccgtctaaa aaggtgatgt gtatttgagt
3601 aaaacagctt gcgtcatgcg gtcgctgcgt atatgatgcg atgagtaaat aaacaaatac
3661 gcaaggggaa cgcatgaagg ttatcgctgt acttaaccag aaaggcgggt caggcaagac
3721 gaccatcgca acccatctag cccgcgccct gcaactcgcc ggggccgatg ttctgttagt
3781 cgattccgat ccccagggca gtgcccgcga ttgggcggcc gtgcgggaag atcaaccgct
3841 aaccgttgtc ggcatcgacc gcccgacgat tgaccgcgac gtgaaggcca tcggccggcg
3901 cgacttcgta gtgatcgacg gagcgcccca ggcggcggac ttggctgtgt ccgcgatcaa
3961 ggcagccgac ttcgtgctga ttccggtgca gccaagccct tacgacatat gggccaccgc
4021 cgacctggtg gagctggtta agcagcgcat tgaggtcacg gatggaaggc tacaagcggc
4081 ctttgtcgtg tcgcgggcga tcaaaggcac gcgcatcggc ggtgaggttg ccgaggcgct
4141 ggccgggtac gagctgccca ttcttgagtc ccgtatcacg cagcgcgtga gctacccagg
4201 cactgccgcc gccggcacaa ccgttcttga atcagaaccc gagggcgacg ctgcccgcga
4261 ggtccaggcg ctggccgctg aaattaaatc aaaactcatt tgagttaatg aggtaaagag
4321 aaaatgagca aaagcacaaa cacgctaagt gccggccgtc cgagcgcacg cagcagcaag
4381 gctgcaacgt tggccagcct ggcagacacg ccagccatga agcgggtcaa ctttcagttg
4441 ccggcggagg atcacaccaa gctgaagatg tacgcggtac gccaaggcaa gaccattacc
4501 gagctgctat ctgaatacat cgcgcagcta ccagagtaaa tgagcaaatg aataaatgag
4561 tagatgaatt ttagcggcta aaggaggcgg catggaaaat caagaacaac caggcaccga
4621 cgccgtggaa tgccccatgt gtggaggaac gggcggttgg ccaggcgtaa gcggctgggt
4681 tgtctgccgg ccctgcaatg gcactggaac ccccaagccc gaggaatcgg cgtgacggtc
4741 gcaaaccatc cggcccggta caaatcggcg cggcgctggg tgatgacctg gtggagaagt
4801 tgaaggccgc gcaggccgcc cagcggcaac gcatcgaggc agaagcacgc cccggtgaat
4861 cgtggcaagc ggccgctgat cgaatccgca aagaatcccg gcaaccgccg gcagccggtg
4921 cgccgtcgat taggaagccg cccaagggcg acgagcaacc agattttttc gttccgatgc
4981 tctatgacgt gggcacccgc gatagtcgca gcatcatgga cgtggccagt tttaaagagt
5041 tttaggcgga aaaatcgcct tttttctctt ttatatcagt cacttacatg tgtgaccggt
5101 tcccaatgta cggctttggg ttcccaatgt acgggttccg gttcccaatg tacggctttg
5161 ggttcccaat gtacgtgcta tccacaggaa agagaccttt tcgacctttt tcccctgcta
5221 gggcaatttg ccctagcatc tgctccgtac attaggaacc ggcggatgct tcgccctcga
5281 tcaggttgcg gtagcgcatg actaggatcg ggccagcctg ccccgcctcc tccttcaaat
5341 cgtactccgg caggtcattt gacccgatca gcttgcgcac ggtgaaacag aacttcttga
5401 actctccggc gctgccactg cgttcgtaga tcgtcttgaa caaccatctg gcttctgcct
5461 tgcctgcggc gcggcgtgcc aggcggtaga gaaaacggcc gatgccggga tcgatcaaaa
5521 agtaatcggg gtgaaccgtc agcacgtccg ggttcttgcc ttctgtgatc tcgcggtaca
5581 tccaatcagc tagctcgatc tcgatgtact ccggccgccc ggtttcgctc tttacgatct
5641 tgtagcggct aatcaaggct tcaccctcgg ataccgtcac caggcggccg ttcttggcct
5701 tcttcgtacg ctgcatggca acgtgcgtgg tgtttaaccg aatgcaggtt tctaccaggt
5761 cgtctttctg ctttccgcca tcggctcgcc ggcagaactt gagtacgtcc gcaacgtgtg
5821 gacggaacac gcggccgggc ttgtctccct tcccttcccg gtatcggttc atggattcgg
5881 ttagatggga aaccgccatc agtaccaggt cgtaatccca cacactggcc atgccggccg
5941 gccctgcgga aacctctacg tgcccgtctg gaagctcgta gcggatcacc tcgccagctc
6001 gtcggtcacg cttcgacaga cggaaaacta ttaaaactct taaaacccgc ctggcctgtg
6061 cataactgtc tggccagcgc acagccgaag agctgcaaaa agcgcctacc cttcggtcgc
6121 tgcgctccct acgccccgcc gcttcgcgtc ggcctatcgc ggccgctggc cgctcaaaaa
6181 tggctggcct acggccaggc aatctaccag ggcgcggaca agccgcgccg tcgccactcg
6241 accgccggcg cccacatcaa ggcaccctgc ctcgcgcgtt tcggtgatga cggtgaaaac
6301 ctctgacaca tgcagctccc ggagacggtc acagcttgtc tgtaagcgga tgccgggagc
6361 agacaagccc gtcagggcgc gtcagcgggt gttggcgggt gtcggggcgc agccatgacc
6421 cagtcacgta gcgatagcgg agtgtatact ggcttaacta tgcggcatca gagcagattg
6481 tactgagagt gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc
6541 gcatcaggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
6601 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
6661 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
6721 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
6781 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
6841 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
6901 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
6961 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
7021 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
7081 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
7141 tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc
7201 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
7261 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
7321 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
7381 aagggatttt ggtcatgcat tctaggtact aaaacaattc atccagtaaa atataatatt
7441 ttattttctc ccaatcaggc ttgatcccca gtaagtcaaa aaatagctcg acatactgtt
7501 cttccccgat atcctccctg atcgaccgga cgcagaaggc aatgtcatac cacttgtccg
7561 ccctgccgct tctcccaaga tcaataaagc cacttacttt gccatctttc acaaagatgt
7621 tgctgtctcc caggtcgccg tgggaaaaga caagttcctc ttcgggcttt tccgtcttta
7681 aaaaatcata cagctcgcgc ggatctttaa atggagtgtc ttcttcccag ttttcgcaat
7741 ccacatcggc cagatcgtta ttcagtaagt aatccaattc ggctaagcgg ctgtctaagc
7801 tattcgtata gggacaatcc gatatgtcga tggagtgaaa gagcctgatg cactccgcat
7861 acagctcgat aatcttttca gggctttgtt catcttcata ctcttccgag caaaggacgc
7921 catcggcctc actcatgagc agattgctcc agccatcatg ccgttcaaag tgcaggacct
7981 ttggaacagg cagctttcct tccagccata gcatcatgtc cttttcccgt tccacatcat
8041 aggtggtccc tttataccgg ctgtccgtca tttttaaata taggttttca ttttctccca
8101 ccagcttata taccttagca ggagacattc cttccgtatc ttttacgcag cggtattttt
8161 cgatcagttt tttcaattcc ggtgatattc tcattttagc catttattat ttccttcctc
8221 ttttctacag tatttaaaga taccccaaga agctaattat aacaagacga actccaattc
8281 actgttcctt gcattctaaa accttaaata ccagaaaaca gctttttcaa agttgttttc
8341 aaagttggcg tataacatag tatcgacgga gccgattttg aaaccgcggt gatcacaggc
8401 agcaacgctc tgtcatcgtt acaatcaaca tgctaccctc cgcgagatca tccgtgtttc
8461 aaacccggca gcttagttgc cgttcttccg aatagcatcg gtaacatgag caaagtctgc
8521 cgccttacaa cggctctccc gctgacgccg tcccggactg atgggctgcc tgtatcgagt
8581 ggtgattttg tgccgagctg ccggtcgggg agctgttggc tggctggtgg caggatatat
8641 tgtggtgtaa acaaattgac gcttagacaa cttaataaca cattgcggac gtttttaatg
8701 tactgaatta acgccgaatt aattcggggg atctggattt tagtactgga ttttggtttt
8761 aggaattaga aattttattg atagaagtat tttacaaata caaatacata ctaagggttt
8821 cttatatgct caacacatga gcgaaaccct ataggaaccc taattccctt atctgggaac
8881 tactcacaca ttattatgga gaaactcgag cttgtcgatc gacagatccg gtcggcatct
8941 actctatttc tttgccctcg gacgagtgct ggggcgtcgg tttccactat cggcgagtac
9001 ttctacacag ccatcggtcc agacggccgc gcttctgcgg gcgatttgtg tacgcccgac
9061 agtcccggct ccggatcgga cgattgcgtc gcatcgaccc tgcgcccaag ctgcatcatc
9121 gaaattgccg tcaaccaagc tctgatagag ttggtcaaga ccaatgcgga gcatatacgc
9181 ccggagtcgt ggcgatcctg caagctccgg atgcctccgc tcgaagtagc gcgtctgctg
9241 ctccatacaa gccaaccacg gcctccagaa gaagatgttg gcgacctcgt attgggaatc
9301 cccgaacatc gcctcgctcc agtcaatgac cgctgttatg cggccattgt ccgtcaggac
9361 attgttggag ccgaaatccg cgtgcacgag gtgccggact tcggggcagt cctcggccca
9421 aagcatcagc tcatcgagag cctgcgcgac ggacgcactg acggtgtcgt ccatcacagt
9481 ttgccagtga tacacatggg gatcagcaat cgcgcatatg aaatcacgcc atgtagtgta
9541 ttgaccgatt ccttgcggtc cgaatgggcc gaacccgctc gtctggctaa gatcggccgc
9601 agcgatcgca tccatagcct ccgcgaccgg ttgtagaaca gcgggcagtt cggtttcagg
9661 caggtcttgc aacgtgacac cctgtgcacg gcgggagatg caataggtca ggctctcgct
9721 aaactcccca atgtcaagca cttccggaat cgggagcgcg gccgatgcaa agtgccgata
9781 aacataacga tctttgtaga aaccatcggc gcagctattt acccgcagga catatccacg
9841 ccctcctaca tcgaagctga aagcacgaga ttcttcgccc tccgagagct gcatcaggtc
9901 ggagacgctg tcgaactttt cgatcagaaa cttctcgaca gacgtcgcgg tgagttcagg
9961 ctttttcata tctcattgcc ccccgggatc tgcgaaagct cgaggagata gatttgtaga
10021 gagagactgg tgatttcagc gtgtcctctc caaatgaaat gaacttcctt atatagagga
10081 aggtcttgcg aaggatagtg ggattgtgcg tcatccctta cgtcagtgga gatatcacat
10141 caatccactt gctttgaaga cgtggttgga acgtcttctt tttccacgat gctcctcgtg
10201 ggtgggggtc catctttggg accactgtcg gcagaggcat cttgaacgat agcctttcct
10261 ttatcgcaat gatggcattt gtaggtgcca ccttcctttt ctactgtcct tttgatgaag
10321 tgacagatag ctgggcaatg gaatccgagg aggtttcccg atattaccct ttgttgaaaa
10381 gtctcaatag ccctttggtc ttctgagact gtatctttga tattcttgga gtagacgaga
10441 gtgtcgtgct ccaccatgtt atcacatcaa tccacttgct ttgaagacgt ggttggaacg
10501 tcttcttttt ccacgatgct cctcgtgggt gggggtccat ctttgggacc actgtcggca
10561 gaggcatctt gaacgatagc ctttccttta tcgcaatgat ggcatttgta ggtgccacct
10621 tccttttcta ctgtcctttt gatgaagtga cagatagctg ggcaatggaa tccgaggagg
10681 tttcccgata ttaccctttg ttgaaaagtc tcaatagccc tttggtcttc tgagactgta
10741 tctttgatat tcttggagta gacgagagtg tcgtgctcca ccatgtttgg caagctgctc
10801 tagccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac
10861 gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc
10921 actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt
10981 gtgagcggat aacaatttca cacaggaaac agctatgaca tgattacgaa ttcccgatct
11041 agtaacatag atgacaccgc gcgcgataat ttatcctagt ttgcgcgcta tattttgttt
11101 tctatcgcgt attaaatgta taattgcggg actctaatca taaaaaccca tctcataaat
11161 aacgtcatgc attacatgtt aattattaca tgcttaacgt aattcaacag aaattatatg
11221 ataatcatcg caagaccggc aacaggattc aatcttaaga aactttattg ccaaatgttt
11281 gaacgatcgg ggaaattcga gctcattaca ttgatgagaa atattacgta catatacata
11341 catccacgtc gatgcttaga agttacagca tgaaccaggg tttttattga acatgtcgaa
11401 agcgtttata aaacataaac acacatttaa ttacggcaca ttcccaccgg ccgcctatat
11461 attgatcgcg ccggtcaagt attttttttc ccaccttttc agcggacgcc gggtcgatta
11521 gatggggatc gcgctggtga tccgcgacta gtatcgattg aaaatctcga aacagccgtg
11581 tcatagtcaa tcattaggtg ttataggaac aatcaaaggt tttttcaagt gttaatcttc
11641 atactaatat atacagtggg tactctttat ctactgccgt ggaactgtca tatttgatta
11701 tgaaatttta gctctagaaa atatttgatc atcaatgtca agactttatg accttgcaaa
11761 atacatttcc taattgagaa cagggtaaaa ttatgaacta tgcctctgaa ccttcataca
11821 caggcagcac attttttgtt gtaaaattca tcttaatatc agcggaaaga ctggaccaga
11881 gaaagaaaaa gttaagacag gcatatactc ttgatcctct aaaagagatg aggcggtaca
11941 atgatcaacc atgaacatta aagtgatacg tggaacatga gaacacaaat aattgtcact
12001 ggaacataag tacaaaaagg tttgtagtaa cttaccgcta gcagatctgt cgacctcgag
12061 ggtacccggg gatcctctag agtcgacctg cagaagtaac accaaacaac agggtgagca
12121 tcgacaaaag aaacagtacc aagcaaataa atagcgtatg aaggcagggc taaaaaaatc
12181 cacatatagc tgctgcatat gccatcatcc aagtatatca agatcaaaat aattataaaa
12241 catacttgtt tattataata gataggtact caaggttaga gcatatgaat agatgctgca
12301 tatgccatca tgtatatgca tcagtaaaac ccacatcaac atgtatacct atcctagatc
12361 gatatttcca tccatcttaa actcgtaact atgaagatgt atgacacaca catacagttc
12421 caaaattaat aaatacacca ggtagtttga aacagtattc tactccgatc tagaacgaat
12481 gaacgaccgc ccaaccacac cacatcatca caaccaagcg aacaaaaagc atctctgtat
12541 atgcatcagt aaaacccgca tcaacatgta tacctatcct agatcgatat ttccatccat
12601 catcttcaat tcgtaactat gaatatgtat ggcacacaca tacagatcca aaattaataa
12661 atccaccagg tagtttgaaa cagaattcta ctccgatcta gaacgaccgc ccaaccagac
12721 cacatcatca caaccaagac aaaaaaaagc atgaaaagat gacccgacaa acaagtgcac
12781 ggcatatatt gaaataaagg aaaagggcaa accaaaccct atgcaacgaa acaaaaaaaa
12841 tcatgaaatc gatcccgtct gcggaacggc tagagccatc ccaggattcc ccaaagagaa
12901 acactggcaa gttagcaatc agaacgtgtc tgacgtacag gtcgcatccg tgtacgaacg
12961 ctagcagcac ggatctaaca caaacacgga tctaacacaa acatgaacag aagtagaact
13021 accgggccct aaccatggac cggaacgccg atctagagaa ggtagagagg gggggggggg
13081 gaggacgagc ggcgtacctt gaagcggagg tgccgacggg tggatttggg ggagatctgg
13141 ttgtgtgtgt gtgcgctccg aacaacacga ggttggggaa agagggtgtg gagggggtgt
13201 ctatttatta cggcgggcga ggaagggaaa gcgaaggagc ggtgggaaag gaatcccccg
13261 tagctgccgg tgccgtgaga ggaggaggag gccgcctgcc gtgccggctc acgtctgccg
13321 ctccgccacg caatttctgg atgccgacag cggagcaagt ccaacggtgg agcggaactc
13381 tcgagagggg tccagaggca gcgacagaga tgccgtgccg tctgcttcgc ttggcccgac
13441 gcgacgctgc tggttcgctg gttggtgtcc gttagactcg tcgacggcgt ttaacaggct
13501 ggcattatct actcgaaaca agaaaaatgt ttccttagtt tttttaattt cttaaagggt
13561 atttgtttaa tttttagtca ctttatttta ttctatttta tatctaaatt attaaataaa
13621 aaaactaaaa tagagtttta gttttcttaa tttagaggct aaaatagaat aaaatagatg
13681 tactaaaaaa attagtctat aaaaaccatt aaccctaaac cctaaatgga tgtactaata
13741 aaatggatga agtattatat aggtgaagct atttgcaaaa aaaaaggaga acacatgcac
13801 actaaaaaga taaaactgta gagtcctgtt gtcaaaatac tcaattgtcc tttagaccat
13861 gtctaactgt tcatttatat gattctctaa aacactgata ttattgtagt actatagatt
13921 atattattcg tagagtaaag tttaaatata tgtataaaga tagataaact gcacttcaaa
13981 caagtgtgac aaaaaaaata tgtggtaatt ttttataact tagacatgca atgctcatta
14041 tctctagaga ggggcacgac cgggtcacgc tgcactgcag gcatgcaagc ttggcactgg
14101 ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt aatcgccttg
14161 cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc gatcgccctt
14221 cccaacagtt gcgcagcctg aatggcgaat gctagagcag cttgagcttg gatcagattg
14281 tcgtttcccg ccttcagttt agcttcatgg agtcaaagat tcaaatagag gacctaacag
14341 aactcgccgt aaagactggc gaacagttca tacagagtct cttacgactc aatgacaaga
14401 agaaaatctt cgtcaacatg gtggagcacg acacacttgt ctactccaaa aatatcaaag
14461 atacagtctc agaagaccaa agggcaattg agacttttca acaaagggta atatccggaa
14521 acctcctcgg attccattgc ccagctatct gtcactttat tgtgaagata gtggaaaagg
14581 aaggtggctc ctacaaatgc catcattgcg ataaaggaaa ggccatcgtt gaagatgcct
14641 ctgccgacag tggtcccaaa gatggacccc cacccacgag gagcatcgtg gaaaaagaag
14701 acgttccaac cacgtcttca aagcaagtgg attgatgtga tatctccact gacgtaaggg
14761 atgacgcaca atcccactat ccttcgcaag acccttcctc tatataagga agttcatttc
14821 atttggagag aacacggggg actcttgacc atggta
//