LOCUS Exported 5856 bp ds-DNA circular SYN 07-FEB-2013
DEFINITION High-copy episomal vector for galactose-inducible expression of
proteins in Saccharomyces cerevisiae.
KEYWORDS pYES2
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5856)
AUTHORS Invitrogen (Life Technologies)
TITLE Direct Submission
JOURNAL Exported Sunday, September 11, 2016 from MiaoLingPlasmid
http://www.miaolingbio.com
FEATURES Location/Qualifiers
source 1..5856
/organism="synthetic DNA construct"
/lab_host="Saccharomyces cerevisiae"
/mol_type="other DNA"
promoter 2..443
/gene="S. cerevisiae GAL1"
/note="GAL1 promoter"
/note="inducible promoter, regulated by Gal4"
protein_bind 2..119
/bound_moiety="Gal4"
/note="UAS"
/note="upstream activating sequence mediating
Gal4-dependent induction"
promoter 475..493
/note="T7 promoter"
/note="promoter for bacteriophage T7 RNA polymerase"
misc_feature 501..600
/note="MCS"
/note="multiple cloning site"
terminator 609..856
/gene="S. cerevisiae CYC1"
/note="CYC1 terminator"
/note="transcription terminator for CYC1"
rep_origin complement(1097..1685)
/direction=LEFT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(1856..2716)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/note="AmpR"
/note="confers resistance fo ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCDTLLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDESDTTMPVAMPTTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKRSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
CDS complement(2812..3615)
/codon_start=1
/gene="S. cerevisiae URA3"
/product="orotidine-5'-phosphate decarboxylase, required
for uracil biosynthesis"
/note="URA3"
/note="yeast auxotrophic marker, counterselectable with
5-fluoroorotic acid (5-FOA)"
/translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
promoter complement(3616..3831)
/gene="S. cerevisiae URA3"
/note="URA3 promoter"
rep_origin 4435..5315
/note="2u ori"
/note="yeast 2u plasmid origin of replication"
rep_origin complement(5384..5839)
/direction=LEFT
/note="f1 ori"
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
ORIGIN
1 acggattaga agccgccgag cgggtgacag ccctccgaag gaagactctc ctccgtgcgt
61 cctcgtcttc accggtcgcg ttcctgaaac gcagatgtgc ctcgcgccgc actgctccga
121 acaataaaga ttctacaata ctagctttta tggttatgaa gaggaaaaat tggcagtaac
181 ctggccccac aaaccttcaa atgaacgaat caaattaaca accataggat gataatgcga
241 ttagtttttt agccttattt ctggggtaat taatcagcga agcgatgatt tttgatctat
301 taacagatat ataaatgcaa aaactgcata accactttaa ctaatacttt caacattttc
361 ggtttgtatt acttcttatt caaatgtaat aaaagtatca acaaaaaatt gttaatatac
421 ctctatactt taacgtcaag gagaaaaaac cccggatcgg actactagca gctgtaatac
481 gactcactat agggaatatt aagcttggta ccgagctcgg atccactagt aacggccgcc
541 agtgtgctgg aattctgcag atatccatca cactggcggc cgctcgagca tgcatctaga
601 gggccgcatc atgtaattag ttatgtcacg cttacattca cgccctcccc ccacatccgc
661 tctaaccgaa aaggaaggag ttagacaacc tgaagtctag gtccctattt atttttttat
721 agttatgtta gtattaagaa cgttatttat atttcaaatt tttctttttt ttctgtacag
781 acgcgtgtac gcatgtaaca ttatactgaa aaccttgctt gagaaggttt tgggacgctc
841 gaaggcttta atttgcggcc ctgcattaat gaatcggcca acgcgcgggg agaggcggtt
901 tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
961 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
1021 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa gcccaggaac cgtaaaaagg
1081 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
1141 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
1201 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
1261 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
1321 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
1381 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
1441 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
1501 tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc
1561 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
1621 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
1681 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
1741 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt
1801 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc
1861 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg
1921 cctgactccc cgtcgtgtag ataactacga tacgggagcg cttaccatct ggccccagtg
1981 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc
2041 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
2101 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg
2161 ttggcattgc tacaggcatc gtggtgtcac tctcgtcgtt tggtatggct tcattcagct
2221 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta
2281 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg
2341 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga
2401 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt
2461 gcccggcgtc aatacgggat aatagtgtat cacatagcag aactttaaaa gtgctcatca
2521 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt
2581 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt
2641 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga
2701 aatgttgaat actcatactc ttcctttttc aatgggtaat aactgatata attaaattga
2761 agctctaatt tgtgagttta gtatacatgc atttacttat aatacagttt tttagttttg
2821 ctggccgcat cttctcaaat atgcttccca gcctgctttt ctgtaacgtt caccctctac
2881 cttagcatcc cttccctttg caaatagtcc tcttccaaca ataataatgt cagatcctgt
2941 agagaccaca tcatccacgg ttctatactg ttgacccaat gcgtctccct tgtcatctaa
3001 acccacaccg ggtgtcataa tcaaccaatc gtaaccttca tctcttccac ccatgtctct
3061 ttgagcaata aagccgataa caaaatcttt gtcgctcttc gcaatgtcaa cagtaccctt
3121 agtatattct ccagtagata gggagccctt gcatgacaat tctgctaaca tcaaaaggcc
3181 tctaggttcc tttgttactt cttctgccgc ctgcttcaaa ccgctaacaa tacctgggcc
3241 caccacaccg tgtgcattcg taatgtctgc ccattctgct attctgtata cacccgcaga
3301 gtactgcaat ttgactgtat taccaatgtc agcaaatttt ctgtcttcga agagtaaaaa
3361 attgtacttg gcggataatg cctttagcgg cttaactgtg ccctccatgg aaaaatcagt
3421 caagatatcc acatgtgttt ttagtaaaca aattttggga cctaatgctt caactaactc
3481 cagtaattcc ttggtggtac gaacatccaa tgaagcacac aagtttgttt gcttttcgtg
3541 catgatatta aatagcttgg cagcaacagg actaggatga gtagcagcac gttccttata
3601 tgtagctttc gacatgattt atcttcgttt cctgcaggtt tttgttctgt gcagttgggt
3661 taagaatact gggcaatttc atgtttcttc aacactacat atgcgtatat ataccaatct
3721 aagtctgtgc tccttccttc gttcttcctt ctgttcggag attaccgaat caaaaaaatt
3781 tcaaagaaac cgaaatcaaa aaaaagaata aaaaaaaaat gatgaattga attgaaaagc
3841 tagcttatcg atgataagct gtcaaagatg agaattaatt ccacggacta tagactatac
3901 tagatactcc gtctactgta cgatacactt ccgctcaggt ccttgtcctt taacgaggcc
3961 ttaccactct tttgttactc tattgatcca gctcagcaaa ggcagtgtga tctaagattc
4021 tatcttcgcg atgtagtaaa actagctaga ccgagaaaga gactagaaat gcaaaaggca
4081 cttctacaat ggctgccatc attattatcc gatgtgacgc tgcagcttct caatgatatt
4141 cgaatacgct ttgaggagat acagcctaat atccgacaaa ctgttttaca gatttacgat
4201 cgtacttgtt acccatcatt gaattttgaa catccgaacc tgggagtttt ccctgaaaca
4261 gatagtatat ttgaacctgt ataataatat atagtctagc gctttacgga agacaatgta
4321 tgtatttcgg ttcctggaga aactattgca tctattgcat aggtaatctt gcacgtcgca
4381 tccccggttc attttctgcg tttccatctt gcacttcaat agcatatctt tgttaacgaa
4441 gcatctgtgc ttcattttgt agaacaaaaa tgcaacgcga gagcgctaat ttttcaaaca
4501 aagaatctga gctgcatttt tacagaacag aaatgcaacg cgaaagcgct attttaccaa
4561 cgaagaatct gtgcttcatt tttgtaaaac aaaaatgcaa cgcgacgaga gcgctaattt
4621 ttcaaacaaa gaatctgagc tgcattttta cagaacagaa atgcaacgcg agagcgctat
4681 tttaccaaca aagaatctat acttcttttt tgttctacaa aaatgcatcc cgagagcgct
4741 atttttctaa caaagcatct tagattactt tttttctcct ttgtgcgctc tataatgcag
4801 tctcttgata actttttgca ctgtaggtcc gttaaggtta gaagaaggct actttggtgt
4861 ctattttctc ttccataaaa aaagcctgac tccacttccc gcgtttactg attactagcg
4921 aagctgcggg tgcatttttt caagataaag gcatccccga ttatattcta taccgatgtg
4981 gattgcgcat actttgtgaa cagaaagtga tagcgttgat gattcttcat tggtcagaaa
5041 attatgaacg gtttcttcta ttttgtctct atatactacg tataggaaat gtttacattt
5101 tcgtattgtt ttcgattcac tctatgaata gttcttacta caattttttt gtctaaagag
5161 taatactaga gataaacata aaaaatgtag aggtcgagtt tagatgcaag ttcaaggagc
5221 gaaaggtgga tgggtaggtt atatagggat atagcacaga gatatatagc aaagagatac
5281 ttttgagcaa tgtttgtgga agcggtattc gcaatgggaa gctccacccc ggttgataat
5341 cagaaaagcc ccaaaaacag gaagattgta taagcaaata tttaaattgt aaacgttaat
5401 attttgttaa aattcgcgtt aaatttttgt taaatcagct cattttttaa cgaatagccc
5461 gaaatcggca aaatccctta taaatcaaaa gaatagaccg agatagggtt gagtgttgtt
5521 ccagtttcca acaagagtcc actattaaag aacgtggact ccaacgtcaa agggcgaaaa
5581 agggtctatc agggcgatgg cccactacgt gaaccatcac cctaatcaag ttttttgggg
5641 tcgaggtgcc gtaaagcagt aaatcggaag ggtaaacgga tgcccccatt tagagcttga
5701 cggggaaagc cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg agcgggggct
5761 agggcggtgg gaagtgtagg ggtcacgctg ggcgtaacca ccacacccgc cgcgcttaat
5821 ggggcgctac agggcgcgtg gggatgatcc actagt
//