LOCUS Exported 4456 bp ds-DNA circular SYN 29-NOV-2013
DEFINITION Yeast integrative vector with a HIS3 marker and an MCS derived from
pBLUESCRIPT II.
ACCESSION U03443
KEYWORDS pRS403
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 4456)
AUTHORS Sikorski RS, Hieter P.
TITLE A system of shuttle vectors and yeast host strains designed for
efficient manipulation of DNA in Saccharomyces cerevisiae.
JOURNAL Genetics 1989;122:19-27.
PUBMED 2659436
REFERENCE 2 (bases 1 to 4456)
TITLE Direct Submission
JOURNAL Exported Monday, September 12, 2016 from MiaoLingPlasmid
http://www.miaolingbio.com
FEATURES Location/Qualifiers
source 1..4456
/organism="synthetic DNA construct"
/lab_host="Saccharomyces cerevisiae"
/mol_type="other DNA"
promoter 317..503
/gene="S. cerevisiae HIS3"
/note="HIS3 promoter"
CDS 504..1163
/codon_start=1
/gene="S. cerevisiae HIS3"
/product="imidazoleglycerol-phosphate dehydratase, required
for histidine biosynthesis"
/note="HIS3"
/note="yeast auxotrophic marker"
/translation="MTEQKALVKRITNETKIQIAISLKGGPLAIEHSIFPEKEAEAVAE
QATQSQVINVHTGIGFLDHMIHALAKHSGWSLIVECIGDLHIDDHHTTEDCGIALGQAF
KEALLARGVKRFGSGFAPLDEALSRAVVDLSNRPYAVVELGLQREKVGDLSCEMIPHFL
ESFAEASRITLHVDCLRGKNDHHRSESAFKALAVAIREATSPNGTNDVPSTKGVLM"
rep_origin complement(1423..1878)
/direction=LEFT
/note="f1 ori"
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
CDS complement(1661..2239)
/codon_start=1
/gene="lacZ fragment"
/product="LacZ-alpha fragment of beta-galactosidase"
/note="lacZ-alpha"
/translation="MTMITPSAQLTLTKGNKSWSSTAVAAALELVDPPGCRNSISSLSI
PSTSRGGPVPNSPYSESYYARSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEAR
TDRPSQQLRSLNGEWRDAPCSGALSAAGVVVTRSVTATLASALAPAPFAFFPSFLATFA
GFPRQALNRGLPLGFRFSALRHLDPKKLD"
primer_bind 2023..2039
/note="M13 fwd"
/note="common sequencing primer, one of multiple similar
variants"
promoter 2049..2067
/note="T7 promoter"
/note="promoter for bacteriophage T7 RNA polymerase"
misc_feature 2076..2183
/note="MCS"
/note="pBluescript multiple cloning site"
promoter complement(2196..2214)
/note="T3 promoter"
/note="promoter for bacteriophage T3 RNA polymerase"
primer_bind complement(2235..2251)
/note="M13 rev"
/note="common sequencing primer, one of multiple similar
variants"
protein_bind 2259..2275
/bound_moiety="lac repressor encoded by lacI"
/note="lac operator"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(2283..2313)
/note="lac promoter"
/note="promoter for the E. coli lac operon"
rep_origin complement(2637..3225)
/direction=LEFT
/note="ori"
/note="high-copy-number colE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(3396..4256)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/note="AmpR"
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(4257..4361)
/gene="bla"
/note="AmpR promoter"
ORIGIN
1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
181 accataaatt cccgttttaa gagcttggtg agcgctagga gtcactgcca ggtatcgttt
241 gaacacggca ttagtcaggg aagtcataac acagtccttt cccgcaattt tctttttcta
301 ttactcttgg cctcctctag tacactctat atttttttat gcctcggtaa tgattttcat
361 tttttttttt cccctagcgg atgactcttt ttttttctta gcgattggca ttatcacata
421 atgaattata cattatataa agtaatgtga tttcttcgaa gaatatacta aaaaatgagc
481 aggcaagata aacgaaggca aagatgacag agcagaaagc cctagtaaag cgtattacaa
541 atgaaaccaa gattcagatt gcgatctctt taaagggtgg tcccctagcg atagagcact
601 cgatcttccc agaaaaagag gcagaagcag tagcagaaca ggccacacaa tcgcaagtga
661 ttaacgtcca cacaggtata gggtttctgg accatatgat acatgctctg gccaagcatt
721 ccggctggtc gctaatcgtt gagtgcattg gtgacttaca catagacgac catcacacca
781 ctgaagactg cgggattgct ctcggtcaag cttttaaaga ggccctactg gcgcgtggag
841 taaaaaggtt tggatcagga tttgcgcctt tggatgaggc actttccaga gcggtggtag
901 atctttcgaa caggccgtac gcagttgtcg aacttggttt gcaaagggag aaagtaggag
961 atctctcttg cgagatgatc ccgcattttc ttgaaagctt tgcagaggct agcagaatta
1021 ccctccacgt tgattgtctg cgaggcaaga atgatcatca ccgtagtgag agtgcgttca
1081 aggctcttgc ggttgccata agagaagcca cctcgcccaa tggtaccaac gatgttccct
1141 ccaccaaagg tgttcttatg tagtgacacc gattatttaa agctgcagca tacgatatat
1201 atacatgtgt atatatgtat acctatgaat gtcagtaagt atgtatacga acagtatgat
1261 actgaagatg acaaggtaat gcatcattct atacgtgtca ttctgaacga ggcgcgcttt
1321 ccttttttct ttttgctttt tctttttttt tctcttgaac tcgacggatc tatgcggtgt
1381 gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggaaattgta aacgttaata
1441 ttttgttaaa attcgcgtta aatttttgtt aaatcagctc attttttaac caataggccg
1501 aaatcggcaa aatcccttat aaatcaaaag aatagaccga gatagggttg agtgttgttc
1561 cagtttggaa caagagtcca ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa
1621 ccgtctatca gggcgatggc ccactacgtg aaccatcacc ctaatcaagt tttttggggt
1681 cgaggtgccg taaagcacta aatcggaacc ctaaagggag cccccgattt agagcttgac
1741 ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta
1801 gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg
1861 cgccgctaca gggcgcgtcg cgccattcgc cattcaggct gcgcaactgt tgggaagggc
1921 gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt gctgcaaggc
1981 gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg acggccagtg
2041 agcgcgcgta atacgactca ctatagggcg aattgggtac cgggcccccc ctcgaggtcg
2101 acggtatcga taagcttgat atcgaattcc tgcagcccgg gggatccact agttctagag
2161 cggccgccac cgcggtggag ctccagcttt tgttcccttt agtgagggtt aattgcgcgc
2221 ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca
2281 cacaacatag gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgaggtaa
2341 ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag
2401 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc
2461 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
2521 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
2581 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
2641 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
2701 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
2761 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
2821 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
2881 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
2941 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
3001 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
3061 tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc
3121 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
3181 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
3241 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
3301 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
3361 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
3421 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
3481 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac
3541 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc
3601 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct
3661 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc
3721 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg
3781 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc
3841 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat
3901 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag
3961 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat
4021 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg
4081 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca
4141 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga
4201 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc
4261 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata
4321 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg
4381 ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc
4441 acgaggccct ttcgtc
//