LOCUS Exported 4946 bp ds-DNA circular SYN 19-SEP-2016
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS Untitled 9
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 4946)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Monday, September 19, 2016 from SnapGene Viewer 3.2.1
http://www.miaolingbio.com
FEATURES Location/Qualifiers
source 1..4946
/organism="synthetic DNA construct"
/mol_type="other DNA"
CDS 79..795
/codon_start=1
/product="enhanced blue variant of GFP"
/note="EBFP"
/note="mammalian codon-optimized"
/translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
FICTTGKLPVPWPTLVTTLTHGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYIMADKQKNGIKV
NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
FVTAAGITLGMDELYK"
CDS 802..828
/codon_start=1
/product="HA (human influenza hemagglutinin) epitope tag"
/note="HA"
/translation="YPYDVPDYA"
terminator 861..1048
/gene="S. cerevisiae ADH1"
/note="ADH1 terminator"
/note="transcription terminator for the S. cerevisiae
alcohol dehydrogenase 1 (ADH1) gene"
gene 1123..2479
/note="kanMX"
/note="yeast selectable marker conferring kanamycin
resistance (Wach et al., 1994)"
promoter 1123..1466
/note="TEF promoter"
/note="Ashbya gossypii TEF promoter"
CDS 1467..2276
/codon_start=1
/gene="aph(3')-Ia"
/product="aminoglycoside phosphotransferase"
/note="KanR"
/note="confers resistance to kanamycin"
/translation="MGKEKTHVSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
terminator 2282..2479
/note="TEF terminator"
/note="Ashbya gossypii TEF terminator"
promoter complement(2584..2602)
/note="T7 promoter"
/note="promoter for bacteriophage T7 RNA polymerase"
rep_origin complement(2860..3448)
/direction=LEFT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(3619..4479)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/note="AmpR"
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(4480..4584)
/gene="bla"
/note="AmpR promoter"
promoter 4930..2
/note="SP6 promoter"
/note="promoter for bacteriophage SP6 RNA polymerase"
ORIGIN
1 gaacgcggcc gccagctgaa gcttcgtacg ctgcaggtcg acggatccgg agcaggtgct
61 ggtgctggtg ctggagcaat gagcaagggc gaggagctgt tcaccggggt ggtgcccatc
121 ctggtcgagc tggacggcga cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag
181 ggcgatgcca cctacggcaa gctgaccctg aagttcatct gcaccaccgg caagctgccc
241 gtgccctggc ccaccctcgt gaccaccctg acccacggcg tgcagtgctt cagccgctac
301 cccgaccaca tgaagcagca cgacttcttc aagtccgcca tgcccgaagg ctacgtccag
361 gagcgcacca tcttcttcaa ggacgacggc aactacaaga cccgcgccga ggtgaagttc
421 gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc
481 aacatcctgg ggcacaagct ggagtacaac ttcaacagcc acaacgtcta tatcatggcc
541 gacaagcaga agaacggcat caaggtgaac ttcaagatcc gccacaacat cgaggacggc
601 agcgtgcagc tcgccgacca ctaccagcag aacaccccca tcggcgacgg ccccgtgctg
661 ctgcccgaca accactacct gagcacccag tccgccctga gcaaagaccc caacgagaag
721 cgcgatcaca tggtcctgct ggagttcgtg accgccgccg ggatcactct cggcatggac
781 gagctgtaca agtaaggatc ctatccatat gacgttccag attacgctgc tcagtgctag
841 ggcgcgccac ttctaaataa gcgaatttct tatgatttat gatttttatt attaaataag
901 ttataaaaaa aataagtgta tacaaatttt aaagtgactc ttaggtttta aaacgaaaat
961 tcttattctt gagtaactct ttcctgtagg tcaggttgct ttctcaggta tagtatgagg
1021 tcgctcttat tgaccacacc tctaccggca gatccgctag ggataacagg gtaatataga
1081 tctgtttagc ttgcctcgtc cccgccgggt cacccggcca gcgacatgga ggcccagaat
1141 accctccttg acagtcttga cgtgcgcagc tcaggggcat gatgtgactg tcgcccgtac
1201 atttagccca tacatcccca tgtataatca tttgcatcca tacattttga tggccgcacg
1261 gcgcgaagca aaaattacgg ctcctcgctg cagacctgcg agcagggaaa cgctcccctc
1321 acagacgcgt tgaattgtcc ccacgccgcg cccctgtaga gaaatataaa aggttaggat
1381 ttgccactga ggttcttctt tcatatactt ccttttaaaa tcttgctagg atacagttct
1441 cacatcacat ccgaacataa acaaccatgg gtaaggaaaa gactcacgtt tcgaggccgc
1501 gattaaattc caacatggat gctgatttat atgggtataa atgggctcgc gataatgtcg
1561 ggcaatcagg tgcgacaatc tatcgattgt atgggaagcc cgatgcgcca gagttgtttc
1621 tgaaacatgg caaaggtagc gttgccaatg atgttacaga tgagatggtc agactaaact
1681 ggctgacgga atttatgcct cttccgacca tcaagcattt tatccgtact cctgatgatg
1741 catggttact caccactgcg atccccggca aaacagcatt ccaggtatta gaagaatatc
1801 ctgattcagg tgaaaatatt gttgatgcgc tggcagtgtt cctgcgccgg ttgcattcga
1861 ttcctgtttg taattgtcct tttaacagcg atcgcgtatt tcgtctcgct caggcgcaat
1921 cacgaatgaa taacggtttg gttgatgcga gtgattttga tgacgagcgt aatggctggc
1981 ctgttgaaca agtctggaaa gaaatgcata agcttttgcc attctcaccg gattcagtcg
2041 tcactcatgg tgatttctca cttgataacc ttatttttga cgaggggaaa ttaataggtt
2101 gtattgatgt tggacgagtc ggaatcgcag accgatacca ggatcttgcc atcctatgga
2161 actgcctcgg tgagttttct ccttcattac agaaacggct ttttcaaaaa tatggtattg
2221 ataatcctga tatgaataaa ttgcagtttc atttgatgct cgatgagttt ttctaatcag
2281 tactgacaat aaaaagattc ttgttttcaa gaacttgtca tttgtatagt ttttttatat
2341 tgtagttgtt ctattttaat caaatgttag cgtgatttat attttttttc gcctcgacat
2401 catctgccca gatgcgaagt taagtgcgca gaaagtaata tcatgcgtca atcgtatgtg
2461 aatgctggtc gctatactgc tgtcgattcg atactaacgc cgccatccag tttaaacgag
2521 ctcgaattca tcgatgatat cagatccact agtggcctat gcggccgcgg atctgccggt
2581 ctccctatag tgagtcgtat taatttcgat aagccaggtt aacctgcatt aatgaatcgg
2641 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga
2701 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
2761 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
2821 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
2881 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
2941 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
3001 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcaatgctc
3061 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
3121 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
3181 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
3241 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
3301 gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
3361 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
3421 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
3481 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
3541 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
3601 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg
3661 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga
3721 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc
3781 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac
3841 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc
3901 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc
3961 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc
4021 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt
4081 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc
4141 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg
4201 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag
4261 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat
4321 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc
4381 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa
4441 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta
4501 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
4561 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga
4621 aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc cctttcgtct
4681 cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg agacggtcac
4741 agcttgtctg taagcggatg ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt
4801 tggcgggtgt cggggctggc ttaactatgc ggcatcagag cagattgtac tgagagtgca
4861 ccatatggac atattgtcgt tagaacgcgg ctacaattaa tacataacct tatgtatcat
4921 acacatacga tttaggtgac actata
//