LOCUS Exported 6200 bp ds-DNA circular SYN 17-SEP-2017
DEFINITION synthetic circular DNA
KEYWORDS pPR3-N
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6200)
AUTHORS yin
TITLE Direct Submission
JOURNAL Exported Sep 17, 2017 from MiaoLingPlasmid
http://www.miaolingbio.com
FEATURES Location/Qualifiers
source 1..6200
/organism="synthetic DNA construct"
/mol_type="other DNA"
misc_feature 64..360
/label=CYC1 promoter
misc_feature 367..369
/label=M
CDS 487..513
/codon_start=1
/product="HA (human influenza hemagglutinin) epitope tag"
/label=HA
/translation="YPYDVPDYA"
misc_feature 514..623
/label=MCS
terminator 630..881
/gene="S. cerevisiae CYC1"
/label=CYC1 terminator
/note="transcription terminator for CYC1"
rep_origin 1081..1536
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
CDS complement(1634..2308)
/codon_start=1
/gene="S. cerevisiae TRP1"
/product="phosphoribosylanthranilate isomerase, required
for tryptophan biosynthesis"
/label=TRP1
/note="yeast auxotrophic marker"
/translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNRK
RTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGDES
WQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSISDW
VGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANFVKNA
KK"
promoter complement(2309..2589)
/gene="S. cerevisiae TRP1"
/label=TRP1 promoter
rep_origin 2844..4186
/label=2u ori
/note="yeast 2u plasmid origin of replication"
promoter 4213..4317
/gene="bla"
/label=AmpR promoter
CDS 4318..5178
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 5349..5937
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 gccgcggatg attacgccaa gcgcgcaatt aaccctcact aaagggaaca aaagctggag
61 ctctcatttg gcgagcgttg gttggtggat caagcccacg cgtaggcaat cctcgagcag
121 atccgccagg cgtgtatata tagcgtggat ggccaggcaa ctttagtgct gacacataca
181 ggcatatata tatgtgtgcg acgacacatg atcatatggc atgcatgtgc tctgtatgta
241 tataaaactc ttgttttctt cttttctcta aatattcttt ccttatacat taggaccttt
301 gcagcataaa ttactatact tctatagaca cgcaaacaca aatacacaca ctaatctaga
361 actagtatgc agattttcgt caagactttg accggtaaaa ccggaacatt ggaagttgaa
421 tcttccgata ccatcgacaa cgttaagtcg aaaattcaag acaaggaagg aatccctggt
481 ggtccatacc catacgatgt tccagattac gctggatcca agcagtggta tcaacgcaga
541 gtggccatta cggcccggga aaaaacatgt cggccgcctc ggcctctcga gaattcgata
601 tcaagcttat cgataccgtc gacctcgagt catgtaatta gttatgtcac gcttacattc
661 acgccctccc cccacatccg ctctaaccga aaaggaagga gttagacaac ctgaagtcta
721 ggtccctatt tattttttta tagttatgtt agtattaaga acgttattta tatttcaaat
781 ttttcttttt tttctgtaca gacgcgtgta cgcatgtaac attatactga aaaccttgct
841 tgagaaggtt ttgggacgct cgaaggcttt aatttgcggc cggtacccaa ttcgccctat
901 agtgagtcgt attacgcgcg ctcactggcc gtcgttttac aacgtcgtga ctgggaaaac
961 cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat
1021 agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg
1081 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg
1141 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca
1201 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta
1261 gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc
1321 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg
1381 gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct tttgatttat
1441 aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta
1501 acgcgaattt taacaaaata ttaacgttta caatttcctg atgcgtattt tctccttacg
1561 catctgtgcg gtatttcaca ccgcataggc aagtgcacaa acaatactta aataaatact
1621 actcagtaat aacctatttc ttagcatttt tgacgaaatt tgctattttg ttagagtctt
1681 ttacaccatt tgtctccaca cctccgctta catcaacacc aataacgcca tttaatctaa
1741 gcgcatcacc aacattttct ggcgtcagtc caccagctaa cataaaatgt aagctttcgg
1801 ggctctcttg ccttccaacc cagtcagaaa tcgagttcca atccaaaagt tcacctgtcc
1861 cacctgcttc tgaatcaaac aagggaataa acgaatgagg tttctgtgaa gctgcactga
1921 gtagtatgtt gcagtctttt ggaaatacga gtcttttaat aactggcaaa ccgaggaact
1981 cttggtattc ttgccacgac tcatctccat gcagttggac gatatcaatg ccgtaatcat
2041 tgaccagagc caaaacatcc tccttaggtt gattacgaaa cacgccaacc aagtatttcg
2101 gagtgcctga actattttta tatgctttta caagacttga aattttcctt gcaataaccg
2161 ggtcaattgt tctctttcta ttgggcacac atataatacc cagcaagtca gcatcggaat
2221 ctagagcaca ttctgcggcc tctgtgctct gcaagccgca aactttcacc aatggaccag
2281 aactacctgt gaaattaata acagacatac tccaagctgc ctttgtgtgc ttaatcacgt
2341 atactcacgt gctcaatagt caccaatgcc ctccctcttg gccctctcct tttctttttt
2401 cgaccgaatt aattcttaat cggcaaaaaa agaaaagctc cggatcaaga ttgtacgtaa
2461 ggtgacaagc tatttttcaa taaagaatat cttccactac tgccatctgg cgtcataact
2521 gcaaagtaca catatattac gatgctgtct attaaatgct tcctatatta tatatatagt
2581 aatgtcgttt atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagc
2641 cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg
2701 cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat
2761 caccgaaacg cgcgagacga aagggcctcg tgatacgcct atttttatag gttaatgtca
2821 tgataataat ggtttcttaa tatgatccaa tatcaaagga aatgatagca ttgaaggatg
2881 agactaatcc aattgaggag tggcagcata tagaacagct aaagggtagt gctgaaggaa
2941 gcatacgata ccccgcatgg aatgggataa tatcacagga ggtactagac tacctttcat
3001 cctacataaa tagacgcata taagtacgca tttaagcata aacacgcact atgccgttct
3061 tctcatgtat atatatatac aggcaacacg cagatatagg tgcgacgtga acagtgagct
3121 gtatgtgcgc agctcgcgtt gcattttcgg aagcgctcgt tttcggaaac gctttgaagt
3181 tcctattccg aagttcctat tctctagaaa gtataggaac ttcagagcgc ttttgaaaac
3241 caaaagcgct ctgaagacgc actttcaaaa aaccaaaaac gcaccggact gtaacgagct
3301 actaaaatat tgcgaatacc gcttccacaa acattgctca aaagtatctc tttgctatat
3361 atctctgtgc tatatcccta tataacctac ccatccacct ttcgctcctt gaacttgcat
3421 ctaaactcga cctctacatt ttttatgttt atctctagta ttactcttta gacaaaaaaa
3481 ttgtagtaag aactattcat agagtgaatc gaaaacaata cgaaaatgta aacatttcct
3541 atacgtagta tatagagaca aaatagaaga aaccgttcat aattttctga ccaatgaaga
3601 atcatcaacg ctatcacttt ctgttcacaa agtatgcgca atccacatcg gtatagaata
3661 taatcgggga tgcctttatc ttgaaaaaat gcacccgcag cttcgctagt aatcagtaaa
3721 cgcgggaagt ggagtcaggc tttttttatg gaagagaaaa tagacaccaa agtagccttc
3781 ttctaacctt aacggaccta cagtgcaaaa agttatcaag agactgcatt atagagcgca
3841 caaaggagaa aaaaagtaat ctaagatgct ttgttagaaa aatagcgctc tcgggatgca
3901 tttttgtaga acaaaaaaga agtatagatt ctttgttggt aaaatagcgc tctcgcgttg
3961 catttctgtt ctgtaaaaat gcagctcaga ttctttgttt gaaaaattag cgctctcgcg
4021 ttgcattttt gttttacaaa aatgaagcac agattcttcg ttggtaaaat agcgctttcg
4081 cgttgcattt ctgttctgta aaaatgcagc tcagattctt tgtttgaaaa attagcgctc
4141 tcgcgttgca tttttgttct acaaaatgaa gcacagatgc ttcgttcagg tggcactttt
4201 cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat
4261 ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg
4321 agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt
4381 tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga
4441 gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa
4501 gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt
4561 attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt
4621 gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc
4681 agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga
4741 ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat
4801 cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct
4861 gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc
4921 cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg
4981 gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc
5041 ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg
5101 acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca
5161 ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta
5221 aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc
5281 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa
5341 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
5401 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
5461 actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc
5521 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
5581 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta
5641 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
5701 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt
5761 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
5821 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
5881 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
5941 gccagcaacg cggcttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
6001 ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata
6061 ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc
6121 gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg
6181 acaggtttcc cgactggaaa
//