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产品名称：p53-ABAI
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简单介绍

p53-ABAI菌株发货前均经过严格的多重验证，如存在质量问题，请在收到产品的三个月内通知我司。p53-ABAI收到质粒后请短暂离心，加入20μl ddH2O溶解质粒，取2μl转化至对应感受态中，挑取单克隆重新提取质粒后使用。

产品描述

p53-ABAI

产品信息
基本信息
质粒简介
质粒图谱
质粒序列

产品信息

产品货号	产品名称	产品规格	优惠价
XY0233	p53-ABAI	5μg	¥请询价

使用说明

信裕质粒平台的各批次质粒菌株发货前均经过严格的多重验证，如存在质量问题，请在收到产品的三个月内通知我司。收到质粒后请短暂离心，加入20μl ddH2O溶解质粒，取2μl转化至对应感受态中，挑取单克隆重新提取质粒后使用。如需获取其他详细信息请登录我司官网查询。

基本信息

启动子:	URA3
复制子:	pUC ori
质粒分类:	酵母系列质粒；酵母杂交质粒；单杂交类质粒
质粒大小:	4944bp
原核抗性:	氨苄青霉素Amp（100μg/ml）
筛选标记:	URA3
克隆菌株:	DH5α等大肠杆菌
培养条件:	37℃，有氧 LB
表达宿主:	Y1Hgold等酵母菌
培养条件:	30℃，YPDA，有氧
5'测序引物:	pABAI-F（GTTCCTTATATGTAGCTTTCGACA）
3'测序引物:	pABAI-R（CCATCTCGAAAAAGGGTTTGCC）
备注:	低拷贝质粒

质粒简介

  p53-ABAI质粒是一种单杂交酵母阳性对照质粒。这个质粒具有AbA抗性因而具有很强的筛选能力，可极大地降低背景水平。
p53-AbAi is a yeast reporter vector that serves as a positive control in the Matchmaker Gold Yeast One-Hybrid Library Screening System. The vector contains a p53 binding site, located upstream of the yeast iso-1-cytochrome C minimal promoter and the AUR1-C gene, an antibiotic resistance gene that confers resistance to Aureobasidin A. Expression of AUR1-C, and thus AbA resistance, is induced by the binding of GAL4 activation domain-p53 fusion proteins to the p53 binding site upstream of AUR1-C.
  p53-AbAi cannot be propagated episomally in yeast; it can only be stably maintained through integration into the host genome. Integration is accomplished via homologous recombination between the vector’s URA3 gene and the nonfunctional ura3-52 locus of the yeast strain provided in the Matchmaker Gold Yeast One-Hybrid System. URA3 is a nutritional marker that can also be used for the selection of recombinant yeast. To allow propagation and selection in E. coli, the vector also contains a Col E1 origin of replication and an ampicillin resistance gene (Ampr).
    p53-AbAi is a positive control reporter vector that is designed to be used in conjunction with the autonomously replicating pGADT7-Rec vector and the p53 control cDNA provided in the Matchmaker Gold Yeast One-Hybrid Library Screening System. To perform control reactions, first linearize the p53-AbAi vector with BstBI, transform the vector into competent yeast cells, and select for integrants on SD/–Ura medium. Next, cotransform the p53 control cDNA and the SmaI-linearized pGADT7-Rec vector (provided) into competent yeast cells, and select for recombinants on SD/–Leu medium containing AbA (see the protocol in the Matchmaker Gold Yeast One-Hybrid Library Screening System User Manual for details).
    Transformation of yeast with the linearized p53-AbAi vector will result in the integration of the vector into the yeast chromosome. Subsequent cotransformation of the linearized pGADT7-Rec vector and the p53 control cDNA will yield a construct, through the gap-repair method,that will constitutively express a GAL4 AD-p53 fusion protein. GAL4 AD-p53 will interact with the p53 binding sites on p53-AbAi and stimulate transcription of AUR1-C.
•    Suitable host strains: DH5α and other general purpose strains.
•    Selectable marker: plasmid confers resistance to ampicillin (100 µg/ml) to E. coli hosts.
•    E. coli replication origin: ColE1
•    Copy number: low

质粒图谱

质粒序列

LOCUS Exported 4944 bp ds-DNA circular SYN 10-SEP-2016

DEFINITION synthetic circular DNA

KEYWORDS p53-AbAi

SOURCE synthetic DNA construct

ORGANISM synthetic DNA construct

REFERENCE 1 (bases 1 to 4944)

TITLE Direct Submission

JOURNAL Exported Saturday, September 10, 2016 from MiaoLingPlasmid

http://www.miaolingbio.com

COMMENT Created by Clontech Laboratories Inc. http://www.clontech.com

Cat. No. 630491

FEATURES Location/Qualifiers

source 1..4944

/organism="synthetic DNA construct"

/mol_type="other DNA"

promoter 25..83

/note="p53 binding site"

misc_feature 235..1440

/note="Aur1-C"

/note="Aureobasidin Resistance"

rep_origin 2209..2659

/note="pUC origin"

misc_feature complement(2856..3716)

/note="Amp(R)"

misc_feature complement(3925..4727)

/note="URA3"

ORIGIN

1 aagcttgaat tcgagctcgg taccaggcat gcctagcatg cctaggcatg cctaggcatg

61 cctaggcatg cctaggcatg cctggatccc tcgaggcatg tgctctgtat gtatataaaa

121 ctcttgtttt cttcttttct ctaaatattc tttccttata cattaggtcc tttgtagcat

181 aaattactat acttctatag acacgcaaac acaaatacac acactaaatt aataatggca

241 aacccttttt cgagatggtt tctatcagag agacctccaa actgccatgt agccgattta

301 gaaacaagtt tagatcccca tcaaacgttg ttgaaggtgc aaaaatacaa acccgcttta

361 agcgactggg tgcattacat cttcttggga tccatcatgc tgtttgtgtt cattactaat

421 cccgcacctt ggatcttcaa gatccttttt tattgtttct tgggcacttt attcatcatt

481 ccagctacgt cacagttttt cttcaatgcc ttgcccatcc taacatgggt ggcgctgtat

541 ttcacttcat cgtactttcc agatgaccgc aggcctccta ttactgtcaa agtgttacca

601 gcggtggaaa caattttata cggcgacaat ttaagtgata ttcttgcaac atcgacgaat

661 tcctttttgg acattttagc atggttaccg tacggactat ttcattatgg ggccccattt

721 gtcgttgctg ccatcttatt cgtatttggt ccaccaactg ttttgcaagg ttatgctttt

781 gcatttggtt atatgaacct gtttggtgtt atcatgcaaa atgtctttcc agccgctccc

841 ccatggtata aaattctcta tggattgcaa tcagccaact atgatatgca tggctcgcct

901 ggtggattag ctagaattga taagctactc ggtattaata tgtatactac atgtttttca

961 aattcctccg tcattttcgg tgcttttcct tcactgcatt ccgggtgtgc tactatggaa

1021 gccctgtttt tctgttattg ttttccaaaa ttgaagccct tgtttattgc ttatgtttgc

1081 tggttatggt ggtcaactat gtatctgaca caccattatt ttgtagacct tatggcaggt

1141 tctgtgctgt catacgttat tttccagtac acaaagtaca cacatttacc aattgtagat

1201 acatctcttt tttgcagatg gtcatacact tcaattgaga aatacgatat atcaaagagt

1261 gatccattgg ctgcagattc aaacgatatc gaaagtgtcc ctttgtccaa cttggaactt

1321 gactttgatc ttaatatgac tgatgaaccc agtgtaagcc cttcgttatt tgatggatct

1381 acttctgttt ctcgttcgtc cgccacgtct ataacgtcac taggtgtaaa gagggcttaa

1441 ggaaatccat tatgtactat ttaaaaaaca caaacttttg gatgttcggt ttattctttt

1501 tcttttactt ttttatcatg ggagcctact tcccgttttt cccgatttgg ctacatgaca

1561 tcaaccatat cagcaaaagt gatacgggta ttatttttgc cgctatttct ctgttctcgc

1621 tattattcca accgctgttt ggtctgcttt ctgacaaact cggcctcgac tctagcgaat

1681 taattcgtaa tcatgtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca

1741 cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa

1801 ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag

1861 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc

1921 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct

1981 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg

2041 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc

2101 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga

2161 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct

2221 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg

2281 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag

2341 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat

2401 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac

2461 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac

2521 tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc

2581 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt

2641 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc

2701 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg

2761 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca

2821 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca

2881 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag

2941 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac

3001 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc

3061 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct

3121 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc

3181 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg

3241 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc

3301 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat

3361 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag

3421 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aacacgggat

3481 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg

3541 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca

3601 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga

3661 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc

3721 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata

3781 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg

3841 ccacctgggt aataactgat ataattaaat tgaagctcta atttgtgagt ttagtataca

3901 tgcatttact tataatacag ttttttagtt ttgctggccg catcttctca aatatgcttc

3961 ccagcctgct tttctgtaac gttcaccctc taccttagca tcccttccct ttgcaaatag

4021 tcctcttcca acaataataa tgtcagatcc tgtagagacc acatcatcca cggttctata

4081 ctgttgaccc aatgcgtctc ccttgtcatc taaacccaca ccgggtgtca taatcaacca

4141 atcgtaacct tcatctcttc cacccatgtc tctttgagca ataaagccga taacaaaatc

4201 tttgtcgctc ttcgcaatgt caacagtacc cttagtatat tctccagtag atagggagcc

4261 cttgcatgac aattctgcta acatcaaaag gcctctaggt tcctttgtta cttcttctgc

4321 cgcctgcttc aaaccgctaa caatacctgg gcccaccaca ccgtgtgcat tcgtaatgtc

4381 tgcccattct gctattctgt atacacccgc agagtactgc aatttgactg tattaccaat

4441 gtcagcaaat tttctgtctt cgaagagtaa aaaattgtac ttggcggata atgcctttag

4501 cggcttaact gtgccctcca tggaaaaatc agtcaagata tccacatgtg tttttagtaa

4561 acaaattttg ggacctaatg cttcaactaa ctccagtaat tccttggtgg tacgaacatc

4621 caatgaagca cacaagtttg tttgcttttc gtgcatgata ttaaatagct tggcagcaac

4681 aggactagga tgagtagcag cacgttcctt atatgtagct ttcgacatga tttatcttcg

4741 tttcctgcag gtttttgttc tgtgcagttg ggttaagaat actgggcaat ttcatgtttc

4801 ttcaacacta catatgcgta tatataccaa tctaagtctg tgctccttcc ttcgttcttc

4861 cttctgttcg gagattaccg aatcaaaaaa atttcaagga aaccgaaatc aaaaaaaaga

4921 ataaaaaaaa aatgatgaat tgaa

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产品目录

产品资料

p53-ABAI

p53-ABAI

产品信息

使用说明

基本信息

质粒简介

质粒图谱

质粒序列