LOCUS Exported 4894 bp ds-DNA circular SYN 10-SEP-2016
DEFINITION synthetic circular DNA
KEYWORDS Untitled 21
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 4894)
TITLE Direct Submission
JOURNAL Exported Saturday, September 10, 2016 from MiaoLingPlasmid
http://www.miaolingbio.com
FEATURES Location/Qualifiers
source 1..4894
/organism="synthetic DNA construct"
/mol_type="other DNA"
CDS 185..1390
/codon_start=1
/gene="S. cerevisiae AUR1 with a dominant F158Y mutation"
/product="aureobasidin A-resistant mutant of inositol
phosphorylceramide synthase"
/note="AurR"
/note="confers fungal resistance to Aureobasidin A"
/translation="MANPFSRWFLSERPPNCHVADLETSLDPHQTLLKVQKYKPALSDW
VHYIFLGSIMLFVFITNPAPWIFKILFYCFLGTLFIIPATSQFFFNALPILTWVALYFT
SSYFPDDRRPPITVKVLPAVETILYGDNLSDILATSTNSFLDILAWLPYGLFHYGAPFV
VAAILFVFGPPTVLQGYAFAFGYMNLFGVIMQNVFPAAPPWYKILYGLQSANYDMHGSP
GGLARIDKLLGINMYTTCFSNSSVIFGAFPSLHSGCATMEALFFCYCFPKLKPLFIAYV
CWLWWSTMYLTHHYFVDLMAGSVLSYVIFQYTKYTHLPIVDTSLFCRWSYTSIEKYDIS
KSDPLAADSNDIESVPLSNLELDFDLNMTDEPSVSPSLFDGSTSVSRSSATSITSLGVK
RA"
primer_bind complement(1645..1661)
/note="M13 rev"
/note="common sequencing primer, one of multiple similar
variants"
protein_bind 1669..1685
/bound_moiety="lac repressor encoded by lacI"
/note="lac operator"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(1693..1723)
/note="lac promoter"
/note="promoter for the E. coli lac operon"
protein_bind 1738..1759
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding site"
/note="CAP binding activates transcription in the presence
of cAMP."
rep_origin complement(2047..2635)
/direction=LEFT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(2806..3666)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/note="AmpR"
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(3667..3771)
/gene="bla"
/note="AmpR promoter"
CDS complement(3875..4678)
/codon_start=1
/gene="S. cerevisiae URA3"
/product="orotidine-5'-phosphate decarboxylase, required
for uracil biosynthesis"
/note="URA3"
/note="yeast auxotrophic marker, counterselectable with
5-fluoroorotic acid (5-FOA)"
/translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
promoter complement(4679..4894)
/gene="S. cerevisiae URA3"
/note="URA3 promoter"
ORIGIN
1 aagcttgaat tcgagctcgg tacccgggga tctgtcgacc tcgaggcatg tgctctgtat
61 gtatataaaa ctcttgtttt cttcttttct ctaaatattc tttccttata cattaggtcc
121 tttgtagcat aaattactat acttctatag acacgcaaac acaaatacac acactaaatt
181 aataatggca aacccttttt cgagatggtt tctatcagag agacctccaa actgccatgt
241 agccgattta gaaacaagtt tagatcccca tcaaacgttg ttgaaggtgc aaaaatacaa
301 acccgcttta agcgactggg tgcattacat cttcttggga tccatcatgc tgtttgtgtt
361 cattactaat cccgcacctt ggatcttcaa gatccttttt tattgtttct tgggcacttt
421 attcatcatt ccagctacgt cacagttttt cttcaatgcc ttgcccatcc taacatgggt
481 ggcgctgtat ttcacttcat cgtactttcc agatgaccgc aggcctccta ttactgtcaa
541 agtgttacca gcggtggaaa caattttata cggcgacaat ttaagtgata ttcttgcaac
601 atcgacgaat tcctttttgg acattttagc atggttaccg tacggactat ttcattatgg
661 ggccccattt gtcgttgctg ccatcttatt cgtatttggt ccaccaactg ttttgcaagg
721 ttatgctttt gcatttggtt atatgaacct gtttggtgtt atcatgcaaa atgtctttcc
781 agccgctccc ccatggtata aaattctcta tggattgcaa tcagccaact atgatatgca
841 tggctcgcct ggtggattag ctagaattga taagctactc ggtattaata tgtatactac
901 atgtttttca aattcctccg tcattttcgg tgcttttcct tcactgcatt ccgggtgtgc
961 tactatggaa gccctgtttt tctgttattg ttttccaaaa ttgaagccct tgtttattgc
1021 ttatgtttgc tggttatggt ggtcaactat gtatctgaca caccattatt ttgtagacct
1081 tatggcaggt tctgtgctgt catacgttat tttccagtac acaaagtaca cacatttacc
1141 aattgtagat acatctcttt tttgcagatg gtcatacact tcaattgaga aatacgatat
1201 atcaaagagt gatccattgg ctgcagattc aaacgatatc gaaagtgtcc ctttgtccaa
1261 cttggaactt gactttgatc ttaatatgac tgatgaaccc agtgtaagcc cttcgttatt
1321 tgatggatct acttctgttt ctcgttcgtc cgccacgtct ataacgtcac taggtgtaaa
1381 gagggcttaa ggaaatccat tatgtactat ttaaaaaaca caaacttttg gatgttcggt
1441 ttattctttt tcttttactt ttttatcatg ggagcctact tcccgttttt cccgatttgg
1501 ctacatgaca tcaaccatat cagcaaaagt gatacgggta ttatttttgc cgctatttct
1561 ctgttctcgc tattattcca accgctgttt ggtctgcttt ctgacaaact cggcctcgac
1621 tctagcgaat taattcgtaa tcatgtcata gctgtttcct gtgtgaaatt gttatccgct
1681 cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg
1741 agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct
1801 gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg
1861 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
1921 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
1981 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
2041 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
2101 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
2161 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
2221 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
2281 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
2341 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
2401 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
2461 gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc
2521 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
2581 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
2641 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
2701 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
2761 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat
2821 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc
2881 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat
2941 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag
3001 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg
3061 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc
3121 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca
3181 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg
3241 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc
3301 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta
3361 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc
3421 aacacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg
3481 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc
3541 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc
3601 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat
3661 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag
3721 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc
3781 ccgaaaagtg ccacctgggt aataactgat ataattaaat tgaagctcta atttgtgagt
3841 ttagtataca tgcatttact tataatacag ttttttagtt ttgctggccg catcttctca
3901 aatatgcttc ccagcctgct tttctgtaac gttcaccctc taccttagca tcccttccct
3961 ttgcaaatag tcctcttcca acaataataa tgtcagatcc tgtagagacc acatcatcca
4021 cggttctata ctgttgaccc aatgcgtctc ccttgtcatc taaacccaca ccgggtgtca
4081 taatcaacca atcgtaacct tcatctcttc cacccatgtc tctttgagca ataaagccga
4141 taacaaaatc tttgtcgctc ttcgcaatgt caacagtacc cttagtatat tctccagtag
4201 atagggagcc cttgcatgac aattctgcta acatcaaaag gcctctaggt tcctttgtta
4261 cttcttctgc cgcctgcttc aaaccgctaa caatacctgg gcccaccaca ccgtgtgcat
4321 tcgtaatgtc tgcccattct gctattctgt atacacccgc agagtactgc aatttgactg
4381 tattaccaat gtcagcaaat tttctgtctt cgaagagtaa aaaattgtac ttggcggata
4441 atgcctttag cggcttaact gtgccctcca tggaaaaatc agtcaagata tccacatgtg
4501 tttttagtaa acaaattttg ggacctaatg cttcaactaa ctccagtaat tccttggtgg
4561 tacgaacatc caatgaagca cacaagtttg tttgcttttc gtgcatgata ttaaatagct
4621 tggcagcaac aggactagga tgagtagcag cacgttcctt atatgtagct ttcgacatga
4681 tttatcttcg tttcctgcag gtttttgttc tgtgcagttg ggttaagaat actgggcaat
4741 ttcatgtttc ttcaacacta catatgcgta tatataccaa tctaagtctg tgctccttcc
4801 ttcgttcttc cttctgttcg gagattaccg aatcaaaaaa atttcaagga aaccgaaatc
4861 aaaaaaaaga ataaaaaaaa aatgatgaat tgaa
//