LOCUS Exported 5186 bp ds-DNA circular SYN 25-NOV-2017
DEFINITION synthetic circular DNA
FEATURES Location/Qualifiers
source 1..5186
/organism="synthetic DNA construct"
/mol_type="other DNA"
promoter 27..131
/gene="bla"
/label=AmpR promoter
CDS 132..992
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 1163..1751
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
terminator 2378..2397
/label=SUP4 terminator
/note="transcription terminator for the S. cerevisiae SUP4
tRNA gene"
promoter complement(2479..2747)
/label=SNR52 promoter
/note="promoter for the S. cerevisiae small nucleolar RNA
gene SNR52"
promoter 4089..4304
/gene="S. cerevisiae URA3"
/label=URA3 promoter
CDS 4305..5108
/codon_start=1
/gene="S. cerevisiae URA3"
/product="orotidine-5'-phosphate decarboxylase, required
for uracil biosynthesis"
/label=URA3
/note="yeast auxotrophic marker, counterselectable with
5-fluoroorotic acid (5-FOA)"
/translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
ORIGIN
1 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
61 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
121 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
181 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
241 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
301 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
361 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc
421 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
481 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
541 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
601 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
661 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
721 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
781 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
841 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
901 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
961 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
1021 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
1081 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
1141 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
1201 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
1261 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt
1321 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
1381 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
1441 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
1501 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
1561 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
1621 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
1681 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
1741 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
1801 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
1861 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
1921 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
1981 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta
2041 atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta
2101 tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt
2161 acgccaagct cgaaattaac cctcactaaa gggaacaaaa gctggtaccg ggccccccct
2221 cgatcgaggt cgacggtatc gataagcttg atatcgaatt cctgcagccc ccgctcgagc
2281 gggggggatc cactagttct agagcggccg tggtatcgtt tagattggca attacagtgt
2341 cttagctcac atgcttataa ctaattacat gactcgaaga cataaaaaac aaaaaaagca
2401 ccaccgactc ggtgccactt tttcaagttg ataacggact agccttattt taacttgcta
2461 tttaaatgca tcgattctga tcatttatct ttcactgcgg agaagtttcg aacgccgaaa
2521 catgcgcacc aactttcact tctacagcgt ttgaccaaaa tcttttgaac agaacattgt
2581 agggtgtgaa aaaatgcgca cctttaccgc tagcccaaga gggcactaca aaatctagag
2641 ttgtacttca aacgtacatg taatcacctt gtatatactc gaaagaaaac atcaagtttc
2701 tgtataaata tgagtgaaag cataatcata cattatcttt tcaaagattg tccgcggtgg
2761 agctccaatt cgccctatag tgagtcgtat tacaattcac tggccgtcgt tttacaacgt
2821 cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc
2881 gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc
2941 ctgaatggcg aatggcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca
3001 caccgcataa ctagctttta acttgtatcc taggttatct atgctgtctc accatagaga
3061 atattaccta tttcagaatg tatgtccatg attcgccggg taaatacata taatacacaa
3121 atctggctta ataaagtcta taatatatct cataaagaag tgctaaattg gctagtgcta
3181 tatattttta agaaaatttc ttttgactaa gtccatatcg actttgtaaa agttcacttt
3241 agcatacata tattacacga gccagaaatt gtaacttttg cctaaaatca caaattgcaa
3301 aatttaattg cttgcaaaag gtcacatgct tataatcaac ttttttaaaa atttaaaata
3361 cttttttatt ttttattttt aaacataaat gaaataattt atttattgtt tatgattacc
3421 gaaacataaa acctgctcaa gaaaaagaaa ctgttttgtc cttggaaaaa aagcactacc
3481 taggagcggc caaaatgccg aggctttcat agcttaaact ctttacagaa aataggcatt
3541 atagatcagt tcgagttttc ttattcttcc ttccggtttt atcgtcacag ttttacagta
3601 aataagtatc acctcttaga gttggcggtt gtttgcaaga ccgagaaaag gctagcaaga
3661 atcgggtcat tgtagcgtat gcgcctgtga acattctctt caacaagttt gattccattg
3721 cggtgaaatg gtaaaagtca accccctgcg atgtatattt tcctgtacaa tcaatcaaaa
3781 agccaaatga tttagcatta tctttacatc ttgttatttt acagatttta tgtttagatc
3841 ttttatgctt gcttttcaaa aggcctgcag gcaagtgcac aaacaatact taaataaata
3901 ctactcagta ataacctatt tcttagcatt tttgacgaaa tttgctattt tgttagagtc
3961 ttttacacca tttgtctcca cacctccgct tacatcaaca ccaataacgc catttaatct
4021 aagcgcatca ccaacatttt ctggcgtcag tccaccagct aacataaaat gtaagctaag
4081 cttttcaatt caattcatca tttttttttt attctttttt ttgatttcgg tttctttgaa
4141 atttttttga ttcggtaatc tccgaacaga aggaagaacg aaggaaggag cacagactta
4201 gattggtata tatacgcata tgtagtgttg aagaaacatg aaattgccca gtattcttaa
4261 cccaactgca cagaacaaaa acctgcagga aacgaagata aatcatgtcg aaagctacat
4321 ataaggaacg tgctgctact catcctagtc ctgttgctgc caagctattt aatatcatgc
4381 acgaaaagca aacaaacttg tgtgcttcat tggatgttcg taccaccaag gaattactgg
4441 agttagttga agcattaggt cccaaaattt gtttactaaa aacacatgtg gatatcttga
4501 ctgatttttc catggagggc acagttaagc cgctaaaggc attatccgcc aagtacaatt
4561 ttttactctt cgaagacaga aaatttgctg acattggtaa tacagtcaaa ttgcagtact
4621 ctgcgggtgt atacagaata gcagaatggg cagacattac gaatgcacac ggtgtggtgg
4681 gcccaggtat tgttagcggt ttgaagcagg cggcagaaga agtaacaaag gaacctagag
4741 gccttttgat gttagcagaa ttgtcatgca agggctccct atctactgga gaatatacta
4801 agggtactgt tgacattgcg aagagcgaca aagattttgt tatcggcttt attgctcaaa
4861 gagacatggg tggaagagat gaaggttacg attggttgat tatgacaccc ggtgtgggtt
4921 tagatgacaa gggagacgca ttgggtcaac agtatagaac cgtggatgat gtggtctcta
4981 caggatctga cattattatt gttggaagag gactatttgc aaagggaagg gatgctaagg
5041 tagagggtga acgttacaga aaagcaggct gggaagcata tttgagaaga tgcggccagc
5101 aaaactaaaa aactgtatta taagtaaatg catgtatact aaactcacaa attagagctt
5161 caatttaatt atatcagtta ttaccc
//