LOCUS Exported 11240 bp ds-DNA circular SYN 03-DEC-2017
DEFINITION synthetic circular DNA
FEATURES Location/Qualifiers
source 1..11240
/organism="synthetic DNA construct"
/mol_type="other DNA"
CDS 1..4113
/codon_start=1
/product="N-terminal portion of Streptococcus pyogenes Cas9
(Zetsche et al., 2015)"
/label=Cas9
/translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEEKEDILEDIVLTLTLFEDREMIEER
LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
DATLIHQSITGLYETRIDLSQLGGDEGA"
CDS 4114..4134
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
terminator 4162..4349
/gene="S. cerevisiae ADH1"
/label=ADH1 terminator
/note="transcription terminator for the S. cerevisiae
alcohol dehydrogenase 1 (ADH1) gene"
terminator 4467..4486
/label=SUP4 terminator
/note="transcription terminator for the S. cerevisiae SUP4
tRNA gene"
promoter complement(4568..4836)
/label=SNR52 promoter
/note="promoter for the S. cerevisiae small nucleolar RNA
gene SNR52"
rep_origin complement(5308..5896)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(6067..6927)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(6928..7032)
/gene="bla"
/label=AmpR promoter
rep_origin 7059..8401
/label=2u ori
/note="yeast 2u plasmid origin of replication"
promoter 8663..8878
/gene="S. cerevisiae URA3"
/label=URA3 promoter
CDS 8879..9682
/codon_start=1
/gene="S. cerevisiae URA3"
/product="orotidine-5'-phosphate decarboxylase, required
for uracil biosynthesis"
/label=URA3
/note="yeast auxotrophic marker, counterselectable with
5-fluoroorotic acid (5-FOA)"
/translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
rep_origin complement(9813..10268)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 10540..11206
/gene="S. cerevisiae TDH3"
/label=GAP promoter
/note="promoter for glyceraldehyde-3-phosphate
dehydrogenase"
ORIGIN
1 atggacaaga agtattctat cggactggac atcgggacta atagcgtcgg gtgggccgtc
61 atcactgacg agtacaaggt gccctctaag aagttcaagg tgctcgggaa caccgaccgg
121 cattccatca agaaaaatct gatcggagct ctcctctttg attcagggga gaccgctgaa
181 gcaacccgcc tcaagcggac tgctagacgg cggtacacca ggaggaagaa ccggatttgt
241 taccttcaag agatattctc caacgaaatg gcaaaggtcg acgacagctt cttccatagg
301 ctggaagaat cattcctcgt ggaagaggat aagaagcatg aacggcatcc catcttcggt
361 aatatcgtcg acgaggtggc ctatcacgag aaatacccaa ccatctacca tcttcgcaaa
421 aagctggtgg actcaaccga caaggcagac ctccggctta tctacctggc cctggcccac
481 atgattaagt tcagaggcca cttcctgatc gagggcgacc tcaatcctga caatagcgat
541 gtggataaac tgttcatcca gctggtgcag acttacaacc agctctttga agagaacccc
601 atcaatgcaa gcggagtcga tgccaaggcc attctgtcag cccggctgtc aaagagccgc
661 agacttgaga atcttatcgc tcagctgccg ggtgaaaaga aaaatggact gttcgggaac
721 ctgattgctc tttcacttgg gctgactccc aatttcaagt ctaatttcga cctggcagag
781 gatgccaagc tgcaactgtc caaggacacc tatgatgacg atctcgacaa cctcctggcc
841 cagatcggtg accaatacgc cgaccttttc cttgctgcta agaatctttc tgacgccatc
901 ctgctgtctg acattctccg cgtgaacact gaaatcacca aggcccctct ttcagcttca
961 atgattaagc ggtatgatga gcaccaccag gacctgaccc tgcttaaggc actcgtccgg
1021 cagcagcttc cggagaagta caaggaaatc ttctttgacc agtcaaagaa tggatacgcc
1081 ggctacatcg acggaggtgc ctcccaagag gaattttata agtttatcaa acctatcctt
1141 gagaagatgg acggcaccga agagctcctc gtgaaactga atcgggagga tctgctgcgg
1201 aagcagcgca ctttcgacaa tgggagcatt ccccaccaga tccatcttgg ggagcttcac
1261 gccatccttc ggcgccaaga ggacttctac ccctttctta aggacaacag ggagaagatt
1321 gagaaaattc tcactttccg catcccctac tacgtgggac ccctcgccag aggaaatagc
1381 cggtttgctt ggatgaccag aaagtcagaa gaaactatca ctccctggaa cttcgaagag
1441 gtggtggaca agggagccag cgctcagtca ttcatcgaac ggatgactaa cttcgataag
1501 aacctcccca atgagaaggt cctgccgaaa cattccctgc tctacgagta ctttaccgtg
1561 tacaacgagc tgaccaaggt gaaatatgtc accgaaggga tgaggaagcc cgcattcctg
1621 tcaggcgaac aaaagaaggc aattgtggac cttctgttca agaccaatag aaaggtgacc
1681 gtgaagcagc tgaaggagga ctatttcaag aaaattgaat gcttcgactc tgtggagatt
1741 agcggggtcg aagatcggtt caacgcaagc ctgggtacct accatgatct gcttaagatc
1801 atcaaggaca aggattttct ggacaatgag gagaaagagg acatccttga ggacattgtc
1861 ctgactctca ctctgttcga ggaccgggaa atgatcgagg agaggcttaa gacctacgcc
1921 catctgttcg acgataaagt gatgaagcaa cttaaacgga gaagatatac cggatgggga
1981 cgccttagcc gcaaactcat caacggaatc cgggacaaac agagcggaaa gaccattctt
2041 gatttcctta agagcgacgg attcgctaat cgcaacttca tgcaacttat ccatgatgat
2101 tccctgacct ttaaggagga catccagaag gcccaagtgt ctggacaagg tgactcactg
2161 cacgagcata tcgcaaatct ggctggttca cccgctatta agaagggtat tctccagacc
2221 gtgaaagtcg tggacgagct ggtcaaggtg atgggtcgcc ataaaccaga gaacattgtc
2281 atcgagatgg ccagggaaaa ccagactacc cagaagggac agaagaacag cagggagcgg
2341 atgaaaagaa ttgaggaagg gattaaggag ctcgggtcac agatccttaa agagcacccg
2401 gtggaaaaca cccagcttca gaatgagaag ctctatctgt actaccttca aaatggacgc
2461 gatatgtatg tggaccaaga gcttgatatc aacaggctct cagactacga cgtggaccac
2521 atcgtccctc agagcttcct caaagacgac tcaattgaca ataaggtgct gactcgctca
2581 gacaagaacc ggggaaagtc agataacgtg ccctcagagg aagtcgtgaa aaagatgaag
2641 aactattggc gccagcttct gaacgcaaag ctaatcactc agcggaagtt cgacaatctc
2701 actaaggctg agaggggcgg actgagcgaa ctggacaaag caggattcat taaacggcaa
2761 cttgtggaga ctcggcagat tactaaacat gtagcccaaa tccttgactc acgcatgaat
2821 accaagtacg acgaaaacga caaacttatc cgcgaggtga aggtgattac cctgaagtcc
2881 aagctggtca gcgatttcag aaaggacttt caattctaca aagtgcggga gatcaataac
2941 tatcatcatg ctcatgacgc atatctgaat gccgtggtgg gaaccgccct aatcaagaag
3001 tacccaaagc tggaaagcga gttcgtgtac ggagactaca aggtctacga cgtgcgcaag
3061 atgattgcca aatctgagca ggagatcgga aaggccaccg caaagtactt cttctacagc
3121 aacatcatga atttcttcaa gaccgaaatc acccttgcaa acggtgagat ccggaagagg
3181 ccgctcatcg agactaatgg ggagactggc gaaatcgtgt gggacaaggg cagagatttc
3241 gctaccgtgc gcaaagtgct ttctatgcct caagtgaaca tcgtgaagaa aaccgaggtg
3301 caaaccggag gcttttctaa ggaatcaatc ctccccaagc gcaactccga caagctcatt
3361 gcaaggaaga aggattggga ccctaagaag tacggcggat tcgattcacc aactgtggct
3421 tattctgtcc tggtcgtggc taaggtggaa aaaggaaagt ctaagaagct caagagcgtg
3481 aaggaactgc tgggtatcac cattatggag cgcagctcct tcgagaagaa cccaattgac
3541 tttctcgaag ccaaaggtta caaggaagtc aagaaggacc ttatcatcaa gctcccaaag
3601 tatagcctgt tcgaactgga gaatgggcgg aagcggatgc tcgcctccgc tggcgaactt
3661 cagaagggta atgagctggc tctcccctcc aagtacgtga atttcctcta ccttgcaagc
3721 cattacgaga agctgaaggg gagccccgag gacaacgagc aaaagcaact gtttgtggag
3781 cagcataagc attatctgga cgagatcatt gagcagattt ccgagttttc taaacgcgtc
3841 attctcgctg atgccaacct cgataaagtc cttagcgcat acaataagca cagagacaaa
3901 ccaattcggg agcaggctga gaatatcatc cacctgttca ccctcaccaa tcttggtgcc
3961 cctgccgcat tcaagtactt cgacaccacc atcgaccgga aacgctatac ctccaccaaa
4021 gaagtgctgg acgccaccct catccaccag agcatcaccg gactttacga aactcggatt
4081 gacctctcac agctcggagg ggatgaggga gctcccaaga aaaagcgcaa ggtaggtagt
4141 tccggatcct agactctcga ggcgaatttc ttatgattta tgatttttat tattaaataa
4201 gttataaaaa aaataagtgt atacaaattt taaagtgact cttaggtttt aaaacgaaaa
4261 ttcttattct tgagtaactc tttcctgtag gtcaggttgc tttctcaggt atagcatgag
4321 gtcgctctta ttgaccacac ctctaccggc atgccgagca aatgcctgca aatcgctccc
4381 catttctcta gagcggccgt ggtatcgttt agattggcaa ttacagtgtc ttagctcaca
4441 tgcttataac taattacatg actcgaagac ataaaaaaca aaaaaagcac caccgactcg
4501 gtgccacttt ttcaagttga taacggacta gccttatttt aacttgctat ttaaatgcat
4561 cgattctgat catttatctt tcactgcgga gaagtttcga acgccgaaac atgcgcacca
4621 actttcactt ctacagcgtt tgaccaaaat cttttgaaca gaacattgta gggtgtgaaa
4681 aaatgcgcac ctttaccgct agcccaagag ggcactacaa aatctagagt tgtacttcaa
4741 acgtacatgt aatcaccttg tatatactcg aaagaaaaca tcaagtttct gtataaatat
4801 gagtgaaagc ataatcatac attatctttt caaagattgt ccgcggtgga gctccagctt
4861 ttgttccctt tagtgagggt taattgcgcg cttggcgtaa tcatggtcat agctgtttcc
4921 tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg
4981 taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc
5041 cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg
5101 gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc
5161 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
5221 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
5281 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
5341 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
5401 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
5461 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
5521 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
5581 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
5641 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
5701 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg
5761 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
5821 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
5881 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
5941 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
6001 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
6061 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc
6121 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc
6181 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc
6241 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc
6301 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt
6361 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc
6421 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa
6481 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt
6541 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg
6601 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc
6661 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa
6721 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt
6781 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt
6841 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag
6901 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta
6961 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat
7021 aggggttccg cgcacatttc cccgaaaagt gccacctgaa cgaagcatct gtgcttcatt
7081 ttgtagaaca aaaatgcaac gcgagagcgc taatttttca aacaaagaat ctgagctgca
7141 tttttacaga acagaaatgc aacgcgaaag cgctatttta ccaacgaaga atctgtgctt
7201 catttttgta aaacaaaaat gcaacgcgag agcgctaatt tttcaaacaa agaatctgag
7261 ctgcattttt acagaacaga aatgcaacgc gagagcgcta ttttaccaac aaagaatcta
7321 tacttctttt ttgttctaca aaaatgcatc ccgagagcgc tatttttcta acaaagcatc
7381 ttagattact ttttttctcc tttgtgcgct ctataatgca gtctcttgat aactttttgc
7441 actgtaggtc cgttaaggtt agaagaaggc tactttggtg tctattttct cttccataaa
7501 aaaagcctga ctccacttcc cgcgtttact gattactagc gaagctgcgg gtgcattttt
7561 tcaagataaa ggcatccccg attatattct ataccgatgt ggattgcgca tactttgtga
7621 acagaaagtg atagcgttga tgattcttca ttggtcagaa aattatgaac ggtttcttct
7681 attttgtctc tatatactac gtataggaaa tgtttacatt ttcgtattgt tttcgattca
7741 ctctatgaat agttcttact acaatttttt tgtctaaaga gtaatactag agataaacat
7801 aaaaaatgta gaggtcgagt ttagatgcaa gttcaaggag cgaaaggtgg atgggtaggt
7861 tatataggga tatagcacag agatatatag caaagagata cttttgagca atgtttgtgg
7921 aagcggtatt cgcaatattt tagtagctcg ttacagtccg gtgcgttttt ggttttttga
7981 aagtgcgtct tcagagcgct tttggttttc aaaagcgctc tgaagttcct atactttcta
8041 gagaatagga acttcggaat aggaacttca aagcgtttcc gaaaacgagc gcttccgaaa
8101 atgcaacgcg agctgcgcac atacagctca ctgttcacgt cgcacctata tctgcgtgtt
8161 gcctgtatat atatatacat gagaagaacg gcatagtgcg tgtttatgct taaatgcgta
8221 cttatatgcg tctatttatg taggatgaaa ggtagtctag tacctcctgt gatattatcc
8281 cattccatgc ggggtatcgt atgcttcctt cagcactacc ctttagctgt tctatatgct
8341 gccactcctc aattggatta gtctcatcct tcaatgctat catttccttt gatattggat
8401 catactaaga aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc
8461 cctttcgtct cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg
8521 agacggtcac agcttgtctg taagcggatg ccgggagcag acaagcccgt cagggcgcgt
8581 cagcgggtgt tggcgggtgt cggggctggc ttaactatgc ggcatcagag cagattgtac
8641 tgagagtgca ccataccacc ttttcaattc atcatttttt ttttattctt ttttttgatt
8701 tcggtttcct tgaaattttt ttgattcggt aatctccgaa cagaaggaag aacgaaggaa
8761 ggagcacaga cttagattgg tatatatacg catatgtagt gttgaagaaa catgaaattg
8821 cccagtattc ttaacccaac tgcacagaac aaaaacctgc aggaaacgaa gataaatcat
8881 gtcgaaagct acatataagg aacgtgctgc tactcatcct agtcctgttg ctgccaagct
8941 atttaatatc atgcacgaaa agcaaacaaa cttgtgtgct tcattggatg ttcgtaccac
9001 caaggaatta ctggagttag ttgaagcatt aggtcccaaa atttgtttac taaaaacaca
9061 tgtggatatc ttgactgatt tttccatgga gggcacagtt aagccgctaa aggcattatc
9121 cgccaagtac aattttttac tcttcgaaga cagaaaattt gctgacattg gtaatacagt
9181 caaattgcag tactctgcgg gtgtatacag aatagcagaa tgggcagaca ttacgaatgc
9241 acacggtgtg gtgggcccag gtattgttag cggtttgaag caggcggcag aagaagtaac
9301 aaaggaacct agaggccttt tgatgttagc agaattgtca tgcaagggct ccctatctac
9361 tggagaatat actaagggta ctgttgacat tgcgaagagc gacaaagatt ttgttatcgg
9421 ctttattgct caaagagaca tgggtggaag agatgaaggt tacgattggt tgattatgac
9481 acccggtgtg ggtttagatg acaagggaga cgcattgggt caacagtata gaaccgtgga
9541 tgatgtggtc tctacaggat ctgacattat tattgttgga agaggactat ttgcaaaggg
9601 aagggatgct aaggtagagg gtgaacgtta cagaaaagca ggctgggaag catatttgag
9661 aagatgcggc cagcaaaact aaaaaactgt attataagta aatgcatgta tactaaactc
9721 acaaattaga gcttcaattt aattatatca gttattaccc tatgcggtgt gaaataccgc
9781 acagatgcgt aaggagaaaa taccgcatca ggaaattgta agcgttaata ttttgttaaa
9841 attcgcgtta aatttttgtt aaatcagctc attttttaac caataggccg aaatcggcaa
9901 aatcccttat aaatcaaaag aatagaccga gatagggttg agtgttgttc cagtttggaa
9961 caagagtcca ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca
10021 gggcgatggc ccactacgtg aaccatcacc ctaatcaagt tttttggggt cgaggtgccg
10081 taaagcacta aatcggaacc ctaaagggag cccccgattt agagcttgac ggggaaagcc
10141 ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta gggcgctggc
10201 aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg cgccgctaca
10261 gggcgcgtcc attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc
10321 tcttcgctat tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta
10381 acgccagggt tttcccagtc acgacgttgt aaaacgacgg ccagtgagcg cgcgtaatac
10441 gactcactat agggcgaatt gggtaccggg ccccccctcg aggtcgacgg tatcgataag
10501 cttgatatcg aattcagtct actagtcagt tcgagtttat cattatcaat actgccattt
10561 caaagaatac gtaaataatt aatagtagtg attttcctaa ctttatttag tcaaaaaatt
10621 agccttttaa ttctgctgta acccgtacat gcccaaaata gggggcgggt tacacagaat
10681 atataacatc gtaggtgtct gggtgaacag tttattcctg gcatccacta aatataatgg
10741 agcccgcttt ttaagctggc atccagaaaa aaaaagaatc ccagcaccaa aatattgttt
10801 tcttcaccaa ccatcagttc ataggtccat tctcttagcg caactacaga gaacaggggc
10861 acaaacaggc aaaaaacggg cacaacctca atggagtgat gcaacctgcc tggagtaaat
10921 gatgacacaa ggcaattgac ccacgcatgt atctatctca ttttcttaca ccttctatta
10981 ccttctgctc tctctgattt ggaaaaagct gaaaaaaaag gttgaaacca gttccctgaa
11041 attattcccc tacttgacta ataagtatat aaagacggta ggtattgatt gtaattctgt
11101 aaatctattt cttaaacttc ttaaattcta cttttatagt tagtcttttt tttagtttta
11161 aaacaccaag aacttagttt cgaataaaca cacataaaca aacaaaagat ctgatatgga
11221 tcgaattaga tctcgccacc
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