LOCUS Exported 12366 bp ds-DNA circular SYN 03-DEC-2017
DEFINITION synthetic circular DNA
FEATURES Location/Qualifiers
source 1..12366
/organism="synthetic DNA construct"
/mol_type="other DNA"
CDS complement(664..1758)
/codon_start=1
/gene="S. cerevisiae LEU2"
/product="3-isopropylmalate dehydrogenase, required for
leucine biosynthesis"
/label=LEU2
/note="yeast auxotrophic marker"
/translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
IGGAAIDATGVPLPDEALEASKKADAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA
NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
VPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ
LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
GLYEPCHGSAPDLPKNKVNPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
DLGGSNSTTEVGDAVAEEVKKILA"
promoter complement(1759..2166)
/gene="S. cerevisiae LEU2"
/label=LEU2 promoter
rep_origin complement(2467..2922)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 3089..3107
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
promoter 3194..3860
/gene="S. cerevisiae TDH3"
/label=GAP promoter
/note="promoter for glyceraldehyde-3-phosphate
dehydrogenase"
regulatory 3889..3898
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
CDS 3895..7998
/codon_start=1
/product="Cas9 (Csn1) endonuclease from the Streptococcus
pyogenes Type II CRISPR/Cas system"
/label=Cas9
/note="generates RNA-guided double strand breaks in DNA"
/translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
DATLIHQSITGLYETRIDLSQLGGD"
CDS 8008..8028
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
terminator 8056..8243
/gene="S. cerevisiae ADH1"
/label=ADH1 terminator
/note="transcription terminator for the S. cerevisiae
alcohol dehydrogenase 1 (ADH1) gene"
terminator 8361..8380
/label=SUP4 terminator
/note="transcription terminator for the S. cerevisiae SUP4
tRNA gene"
promoter complement(8462..8730)
/label=SNR52 promoter
/note="promoter for the S. cerevisiae small nucleolar RNA
gene SNR52"
rep_origin complement(9202..9790)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(9961..10821)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(10822..10926)
/gene="bla"
/label=AmpR promoter
rep_origin 10953..12299
/label=2u ori
/note="yeast 2u plasmid origin of replication"
ORIGIN
1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
181 accatatcga ctacgtcgtt aaggccgttt ctgacagagt aaaattcttg agggaacttt
241 caccattatg ggaaatggtt caagaaggta ttgacttaaa ctccatcaaa tggtcaggtc
301 attgagtgtt ttttatttgt tgtatttttt tttttttaga gaaaatcctc caatatataa
361 attaggaatc atagtttcat gattttctgt tacacctaac tttttgtgtg gtgccctcct
421 ccttgtcaat attaatgtta aagtgcaatt ctttttcctt atcacgttga gccattagta
481 tcaatttgct tacctgtatt cctttacatc ctcctttttc tccttcttga taaatgtatg
541 tagattgcgt atatagtttc gtctacccta tgaacatatt ccattttgta atttcgtgtc
601 gtttctatta tgaatttcat ttataaagtt tatgtacaaa tatcataaaa aaagagaatc
661 tttttaagca aggattttct taacttcttc ggcgacagca tcaccgactt cggtggtact
721 gttggaacca cctaaatcac cagttctgat acctgcatcc aaaacctttt taactgcatc
781 ttcaatggcc ttaccttctt caggcaagtt caatgacaat ttcaacatca ttgcagcaga
841 caagatagtg gcgatagggt tgaccttatt ctttggcaaa tctggagcag aaccgtggca
901 tggttcgtac aaaccaaatg cggtgttctt gtctggcaaa gaggccaagg acgcagatgg
961 caacaaaccc aaggaacctg ggataacgga ggcttcatcg gagatgatat caccaaacat
1021 gttgctggtg attataatac catttaggtg ggttgggttc ttaactagga tcatggcggc
1081 agaatcaatc aattgatgtt gaaccttcaa tgtaggaaat tcgttcttga tggtttcctc
1141 cacagttttt ctccataatc ttgaagaggc caaaacatta gctttatcca aggaccaaat
1201 aggcaatggt ggctcatgtt gtagggccat gaaagcggcc attcttgtga ttctttgcac
1261 ttctggaacg gtgtattgtt cactatccca agcgacacca tcaccatcgt cttcctttct
1321 cttaccaaag taaatacctc ccactaattc tctgacaaca acgaagtcag tacctttagc
1381 aaattgtggc ttgattggag ataagtctaa aagagagtcg gatgcaaagt tacatggtct
1441 taagttggcg tacaattgaa gttctttacg gatttttagt aaaccttgtt caggtctaac
1501 actacctgta ccccatttag gaccacccac agcacctaac aaaacggcat cagccttctt
1561 ggaggcttcc agcgcctcat ctggaagtgg aacacctgta gcatctatag cagcaccacc
1621 aattaaatga ttttcgaaat cgaacttgac attggaacga acatcagaaa tagctttaag
1681 aaccttaatg gcttcggctg tgatttcttg accaacgtgg tcacctggca aaacgacgat
1741 cttcttaggg gcagacatta gaatggtata tccttgaaat atatatatat atattgctga
1801 aatgtaaaag gtaagaaaag ttagaaagta agacgattgc taaccaccta ttggaaaaaa
1861 caataggtcc ttaaataata ttgtcaactt caagtattgt gatgcaagca tttagtcatg
1921 aacgcttctc tattctatat gaaaagccgg ttccggcgct ctcacctttc ctttttctcc
1981 caatttttca gttgaaaaag gtatatgcgt caggcgacct ctgaaattaa caaaaaattt
2041 ccagtcatcg aatttgattc tgtgcgatag cgcccctgtg tgttctcgtt atgttgagga
2101 aaaaaataat ggttgctaag agattcgaac tcttgcatct tacgatacct gagtattccc
2161 acagttaact gcggtcaaga tatttcttga atcaggcgcc ttagaccgct cggccaaaca
2221 accaattact tgttgagaaa tagagtataa ttatcctata aatataacgt ttttgaacac
2281 acatgaacaa ggaagtacag gacaattgat tttgaagaga atgtggattt tgatgtaatt
2341 gttgggattc catttttaat aaggcaataa tattaggtat gtagatatac tagaagttct
2401 cctcgaccgg tcgatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc
2461 atcaggaaat tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca
2521 gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga
2581 ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg
2641 actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat
2701 caccctaatc aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag
2761 ggagcccccg atttagagct tgacggggaa agccggcgaa cgtggcgaga aaggaaggga
2821 agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa
2881 ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc gtccattcgc cattcaggct
2941 gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa
3001 agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg
3061 ttgtaaaacg acggccagtg agcgcgcgta atacgactca ctatagggcg aattgggtac
3121 cgggcccccc ctcgaggtcg acggtatcga taagcttgat atcgaattca gtctactagt
3181 cagttcgagt ttatcattat caatactgcc atttcaaaga atacgtaaat aattaatagt
3241 agtgattttc ctaactttat ttagtcaaaa aattagcctt ttaattctgc tgtaacccgt
3301 acatgcccaa aatagggggc gggttacaca gaatatataa catcgtaggt gtctgggtga
3361 acagtttatt cctggcatcc actaaatata atggagcccg ctttttaagc tggcatccag
3421 aaaaaaaaag aatcccagca ccaaaatatt gttttcttca ccaaccatca gttcataggt
3481 ccattctctt agcgcaacta cagagaacag gggcacaaac aggcaaaaaa cgggcacaac
3541 ctcaatggag tgatgcaacc tgcctggagt aaatgatgac acaaggcaat tgacccacgc
3601 atgtatctat ctcattttct tacaccttct attaccttct gctctctctg atttggaaaa
3661 agctgaaaaa aaaggttgaa accagttccc tgaaattatt cccctacttg actaataagt
3721 atataaagac ggtaggtatt gattgtaatt ctgtaaatct atttcttaaa cttcttaaat
3781 tctactttta tagttagtct tttttttagt tttaaaacac caagaactta gtttcgaata
3841 aacacacata aacaaacaaa agatctgata tggatcgaat tagatctcgc caccatggac
3901 aagaagtatt ctatcggact ggacatcggg actaatagcg tcgggtgggc cgtcatcact
3961 gacgagtaca aggtgccctc taagaagttc aaggtgctcg ggaacaccga ccggcattcc
4021 atcaagaaaa atctgatcgg agctctcctc tttgattcag gggagaccgc tgaagcaacc
4081 cgcctcaagc ggactgctag acggcggtac accaggagga agaaccggat ttgttacctt
4141 caagagatat tctccaacga aatggcaaag gtcgacgaca gcttcttcca taggctggaa
4201 gaatcattcc tcgtggaaga ggataagaag catgaacggc atcccatctt cggtaatatc
4261 gtcgacgagg tggcctatca cgagaaatac ccaaccatct accatcttcg caaaaagctg
4321 gtggactcaa ccgacaaggc agacctccgg cttatctacc tggccctggc ccacatgatt
4381 aagttcagag gccacttcct gatcgagggc gacctcaatc ctgacaatag cgatgtggat
4441 aaactgttca tccagctggt gcagacttac aaccagctct ttgaagagaa ccccatcaat
4501 gcaagcggag tcgatgccaa ggccattctg tcagcccggc tgtcaaagag ccgcagactt
4561 gagaatctta tcgctcagct gccgggtgaa aagaaaaatg gactgttcgg gaacctgatt
4621 gctctttcac ttgggctgac tcccaatttc aagtctaatt tcgacctggc agaggatgcc
4681 aagctgcaac tgtccaagga cacctatgat gacgatctcg acaacctcct ggcccagatc
4741 ggtgaccaat acgccgacct tttccttgct gctaagaatc tttctgacgc catcctgctg
4801 tctgacattc tccgcgtgaa cactgaaatc accaaggccc ctctttcagc ttcaatgatt
4861 aagcggtatg atgagcacca ccaggacctg accctgctta aggcactcgt ccggcagcag
4921 cttccggaga agtacaagga aatcttcttt gaccagtcaa agaatggata cgccggctac
4981 atcgacggag gtgcctccca agaggaattt tataagttta tcaaacctat ccttgagaag
5041 atggacggca ccgaagagct cctcgtgaaa ctgaatcggg aggatctgct gcggaagcag
5101 cgcactttcg acaatgggag cattccccac cagatccatc ttggggagct tcacgccatc
5161 cttcggcgcc aagaggactt ctaccccttt cttaaggaca acagggagaa gattgagaaa
5221 attctcactt tccgcatccc ctactacgtg ggacccctcg ccagaggaaa tagccggttt
5281 gcttggatga ccagaaagtc agaagaaact atcactccct ggaacttcga agaggtggtg
5341 gacaagggag ccagcgctca gtcattcatc gaacggatga ctaacttcga taagaacctc
5401 cccaatgaga aggtcctgcc gaaacattcc ctgctctacg agtactttac cgtgtacaac
5461 gagctgacca aggtgaaata tgtcaccgaa gggatgagga agcccgcatt cctgtcaggc
5521 gaacaaaaga aggcaattgt ggaccttctg ttcaagacca atagaaaggt gaccgtgaag
5581 cagctgaagg aggactattt caagaaaatt gaatgcttcg actctgtgga gattagcggg
5641 gtcgaagatc ggttcaacgc aagcctgggt acctaccatg atctgcttaa gatcatcaag
5701 gacaaggatt ttctggacaa tgaggagaac gaggacatcc ttgaggacat tgtcctgact
5761 ctcactctgt tcgaggaccg ggaaatgatc gaggagaggc ttaagaccta cgcccatctg
5821 ttcgacgata aagtgatgaa gcaacttaaa cggagaagat ataccggatg gggacgcctt
5881 agccgcaaac tcatcaacgg aatccgggac aaacagagcg gaaagaccat tcttgatttc
5941 cttaagagcg acggattcgc taatcgcaac ttcatgcaac ttatccatga tgattccctg
6001 acctttaagg aggacatcca gaaggcccaa gtgtctggac aaggtgactc actgcacgag
6061 catatcgcaa atctggctgg ttcacccgct attaagaagg gtattctcca gaccgtgaaa
6121 gtcgtggacg agctggtcaa ggtgatgggt cgccataaac cagagaacat tgtcatcgag
6181 atggccaggg aaaaccagac tacccagaag ggacagaaga acagcaggga gcggatgaaa
6241 agaattgagg aagggattaa ggagctcggg tcacagatcc ttaaagagca cccggtggaa
6301 aacacccagc ttcagaatga gaagctctat ctgtactacc ttcaaaatgg acgcgatatg
6361 tatgtggacc aagagcttga tatcaacagg ctctcagact acgacgtgga ccacatcgtc
6421 cctcagagct tcctcaaaga cgactcaatt gacaataagg tgctgactcg ctcagacaag
6481 aaccggggaa agtcagataa cgtgccctca gaggaagtcg tgaaaaagat gaagaactat
6541 tggcgccagc ttctgaacgc aaagctaatc actcagcgga agttcgacaa tctcactaag
6601 gctgagaggg gcggactgag cgaactggac aaagcaggat tcattaaacg gcaacttgtg
6661 gagactcggc agattactaa acatgtcgcc caaatccttg actcacgcat gaataccaag
6721 tacgacgaaa acgacaaact tatccgcgag gtgaaggtga ttaccctgaa gtccaagctg
6781 gtcagcgatt tcagaaagga ctttcaattc tacaaagtgc gggagatcaa taactatcat
6841 catgctcatg acgcatatct gaatgccgtg gtgggaaccg ccctaatcaa gaagtaccca
6901 aagctggaaa gcgagttcgt gtacggagac tacaaggtct acgacgtgcg caagatgatt
6961 gccaaatctg agcaggagat cggaaaggcc accgcaaagt acttcttcta cagcaacatc
7021 atgaatttct tcaagaccga aatcaccctt gcaaacggtg agatccggaa gaggccgctc
7081 atcgagacta atggggagac tggcgaaatc gtgtgggaca agggcagaga tttcgctacc
7141 gtgcgcaaag tgctttctat gcctcaagtg aacatcgtga agaaaaccga ggtgcaaacc
7201 ggaggctttt ctaaggaatc aatcctcccc aagcgcaact ccgacaagct cattgcaagg
7261 aagaaggatt gggaccctaa gaagtacggc ggattcgatt caccaactgt ggcttattct
7321 gtcctggtcg tggctaaggt ggaaaaagga aagtctaaga agctcaagag cgtgaaggaa
7381 ctgctgggta tcaccattat ggagcgcagc tccttcgaga agaacccaat tgactttctc
7441 gaagccaaag gttacaagga agtcaagaag gaccttatca tcaagctccc aaagtatagc
7501 ctgttcgaac tggagaatgg gcggaagcgg atgctcgcct ccgctggcga acttcagaag
7561 ggtaatgagc tggctctccc ctccaagtac gtgaatttcc tctaccttgc aagccattac
7621 gagaagctga aggggagccc cgaggacaac gagcaaaagc aactgtttgt ggagcagcat
7681 aagcattatc tggacgagat cattgagcag atttccgagt tttctaaacg cgtcattctc
7741 gctgatgcca acctcgataa agtccttagc gcatacaata agcacagaga caaaccaatt
7801 cgggagcagg ctgagaatat catccacctg ttcaccctca ccaatcttgg tgcccctgcc
7861 gcattcaagt acttcgacac caccatcgac cggaaacgct atacctccac caaagaagtg
7921 ctggacgcca ccctcatcca ccagagcatc accggacttt acgaaactcg gattgacctc
7981 tcacagctcg gaggggatga gggagctccc aagaaaaagc gcaaggtagg tagttccgga
8041 tcctagactc tcgaggcgaa tttcttatga tttatgattt ttattattaa ataagttata
8101 aaaaaaataa gtgtatacaa attttaaagt gactcttagg ttttaaaacg aaaattctta
8161 ttcttgagta actctttcct gtaggtcagg ttgctttctc aggtatagca tgaggtcgct
8221 cttattgacc acacctctac cggcatgccg agcaaatgcc tgcaaatcgc tccccatttc
8281 tctagagcgg ccgtggtatc gtttagattg gcaattacag tgtcttagct cacatgctta
8341 taactaatta catgactcga agacataaaa aacaaaaaaa gcaccaccga ctcggtgcca
8401 ctttttcaag ttgataacgg actagcctta ttttaacttg ctatttaaat gcatcgattc
8461 tgatcattta tctttcactg cggagaagtt tcgaacgccg aaacatgcgc accaactttc
8521 acttctacag cgtttgacca aaatcttttg aacagaacat tgtagggtgt gaaaaaatgc
8581 gcacctttac cgctagccca agagggcact acaaaatcta gagttgtact tcaaacgtac
8641 atgtaatcac cttgtatata ctcgaaagaa aacatcaagt ttctgtataa atatgagtga
8701 aagcataatc atacattatc ttttcaaaga ttgtccgcgg tggagctcca gcttttgttc
8761 cctttagtga gggttaattg cgcgcttggc gtaatcatgg tcatagctgt ttcctgtgtg
8821 aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc
8881 ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt
8941 ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg
9001 cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt
9061 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc
9121 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa
9181 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
9241 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
9301 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
9361 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag
9421 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
9481 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
9541 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
9601 agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg
9661 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
9721 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
9781 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa
9841 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt
9901 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag
9961 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
10021 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
10081 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa
10141 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
10201 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
10261 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
10321 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc
10381 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact
10441 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc
10501 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
10561 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct
10621 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
10681 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
10741 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
10801 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg
10861 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
10921 tccgcgcaca tttccccgaa aagtgccacc tgaacgaagc atctgtgctt cattttgtag
10981 aacaaaaatg caacgcgaga gcgctaattt ttcaaacaaa gaatctgagc tgcattttta
11041 cagaacagaa atgcaacgcg aaagcgctat tttaccaacg aagaatctgt gcttcatttt
11101 tgtaaaacaa aaatgcaacg cgagagcgct aatttttcaa acaaagaatc tgagctgcat
11161 ttttacagaa cagaaatgca acgcgagagc gctattttac caacaaagaa tctatacttc
11221 ttttttgttc tacaaaaatg catcccgaga gcgctatttt tctaacaaag catcttagat
11281 tacttttttt ctcctttgtg cgctctataa tgcagtctct tgataacttt ttgcactgta
11341 ggtccgttaa ggttagaaga aggctacttt ggtgtctatt ttctcttcca taaaaaaagc
11401 ctgactccac ttcccgcgtt tactgattac tagcgaagct gcgggtgcat tttttcaaga
11461 taaaggcatc cccgattata ttctataccg atgtggattg cgcatacttt gtgaacagaa
11521 agtgatagcg ttgatgattc ttcattggtc agaaaattat gaacggtttc ttctattttg
11581 tctctatata ctacgtatag gaaatgttta cattttcgta ttgttttcga ttcactctat
11641 gaatagttct tactacaatt tttttgtcta aagagtaata ctagagataa acataaaaaa
11701 tgtagaggtc gagtttagat gcaagttcaa ggagcgaaag gtggatgggt aggttatata
11761 gggatatagc acagagatat atagcaaaga gatacttttg agcaatgttt gtggaagcgg
11821 tattcgcaat attttagtag ctcgttacag tccggtgcgt ttttggtttt ttgaaagtgc
11881 gtcttcagag cgcttttggt tttcaaaagc gctctgaagt tcctatactt tctagctaga
11941 gaataggaac ttcggaatag gaacttcaaa gcgtttccga aaacgagcgc ttccgaaaat
12001 gcaacgcgag ctgcgcacat acagctcact gttcacgtcg cacctatatc tgcgtgttgc
12061 ctgtatatat atatacatga gaagaacggc atagtgcgtg tttatgctta aatgcgtact
12121 tatatgcgtc tatttatgta ggatgaaagg tagtctagta cctcctgtga tattatccca
12181 ttccatgcgg ggtatcgtat gcttccttca gcactaccct ttagctgttc tatatgctgc
12241 cactcctcaa ttggattagt ctcatccttc aatgctatca tttcctttga tattggatca
12301 tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc acgaggccct
12361 ttcgtc
//