LOCUS Exported 11928 bp ds-DNA circular SYN 03-DEC-2017
DEFINITION synthetic circular DNA
FEATURES Location/Qualifiers
source 1..11928
/organism="synthetic DNA construct"
/mol_type="other DNA"
CDS complement(664..1758)
/codon_start=1
/gene="S. cerevisiae LEU2"
/product="3-isopropylmalate dehydrogenase, required for
leucine biosynthesis"
/label=LEU2
/note="yeast auxotrophic marker"
/translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
IGGAAIDATGVPLPDEALEASKKADAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA
NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
VPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ
LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
GLYEPCHGSAPDLPKNKVNPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
DLGGSNSTTEVGDAVAEEVKKILA"
promoter complement(1759..2166)
/gene="S. cerevisiae LEU2"
/label=LEU2 promoter
rep_origin complement(2467..2922)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 3194..3860
/gene="S. cerevisiae TDH3"
/label=GAP promoter
/note="promoter for glyceraldehyde-3-phosphate
dehydrogenase"
regulatory 3889..3898
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
CDS 3895..7998
/codon_start=1
/product="Cas9 (Csn1) endonuclease from the Streptococcus
pyogenes Type II CRISPR/Cas system"
/label=Cas9
/note="generates RNA-guided double strand breaks in DNA"
/translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
DATLIHQSITGLYETRIDLSQLGGD"
CDS 8008..8028
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
terminator 8056..8243
/gene="S. cerevisiae ADH1"
/label=ADH1 terminator
/note="transcription terminator for the S. cerevisiae
alcohol dehydrogenase 1 (ADH1) gene"
rep_origin complement(8764..9352)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(9523..10383)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(10384..10488)
/gene="bla"
/label=AmpR promoter
rep_origin 10515..11861
/label=2u ori
/note="yeast 2u plasmid origin of replication"
ORIGIN
1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
181 accatatcga ctacgtcgtt aaggccgttt ctgacagagt aaaattcttg agggaacttt
241 caccattatg ggaaatggtt caagaaggta ttgacttaaa ctccatcaaa tggtcaggtc
301 attgagtgtt ttttatttgt tgtatttttt tttttttaga gaaaatcctc caatatataa
361 attaggaatc atagtttcat gattttctgt tacacctaac tttttgtgtg gtgccctcct
421 ccttgtcaat attaatgtta aagtgcaatt ctttttcctt atcacgttga gccattagta
481 tcaatttgct tacctgtatt cctttacatc ctcctttttc tccttcttga taaatgtatg
541 tagattgcgt atatagtttc gtctacccta tgaacatatt ccattttgta atttcgtgtc
601 gtttctatta tgaatttcat ttataaagtt tatgtacaaa tatcataaaa aaagagaatc
661 tttttaagca aggattttct taacttcttc ggcgacagca tcaccgactt cggtggtact
721 gttggaacca cctaaatcac cagttctgat acctgcatcc aaaacctttt taactgcatc
781 ttcaatggcc ttaccttctt caggcaagtt caatgacaat ttcaacatca ttgcagcaga
841 caagatagtg gcgatagggt tgaccttatt ctttggcaaa tctggagcag aaccgtggca
901 tggttcgtac aaaccaaatg cggtgttctt gtctggcaaa gaggccaagg acgcagatgg
961 caacaaaccc aaggaacctg ggataacgga ggcttcatcg gagatgatat caccaaacat
1021 gttgctggtg attataatac catttaggtg ggttgggttc ttaactagga tcatggcggc
1081 agaatcaatc aattgatgtt gaaccttcaa tgtaggaaat tcgttcttga tggtttcctc
1141 cacagttttt ctccataatc ttgaagaggc caaaacatta gctttatcca aggaccaaat
1201 aggcaatggt ggctcatgtt gtagggccat gaaagcggcc attcttgtga ttctttgcac
1261 ttctggaacg gtgtattgtt cactatccca agcgacacca tcaccatcgt cttcctttct
1321 cttaccaaag taaatacctc ccactaattc tctgacaaca acgaagtcag tacctttagc
1381 aaattgtggc ttgattggag ataagtctaa aagagagtcg gatgcaaagt tacatggtct
1441 taagttggcg tacaattgaa gttctttacg gatttttagt aaaccttgtt caggtctaac
1501 actacctgta ccccatttag gaccacccac agcacctaac aaaacggcat cagccttctt
1561 ggaggcttcc agcgcctcat ctggaagtgg aacacctgta gcatctatag cagcaccacc
1621 aattaaatga ttttcgaaat cgaacttgac attggaacga acatcagaaa tagctttaag
1681 aaccttaatg gcttcggctg tgatttcttg accaacgtgg tcacctggca aaacgacgat
1741 cttcttaggg gcagacatta gaatggtata tccttgaaat atatatatat atattgctga
1801 aatgtaaaag gtaagaaaag ttagaaagta agacgattgc taaccaccta ttggaaaaaa
1861 caataggtcc ttaaataata ttgtcaactt caagtattgt gatgcaagca tttagtcatg
1921 aacgcttctc tattctatat gaaaagccgg ttccggcgct ctcacctttc ctttttctcc
1981 caatttttca gttgaaaaag gtatatgcgt caggcgacct ctgaaattaa caaaaaattt
2041 ccagtcatcg aatttgattc tgtgcgatag cgcccctgtg tgttctcgtt atgttgagga
2101 aaaaaataat ggttgctaag agattcgaac tcttgcatct tacgatacct gagtattccc
2161 acagttaact gcggtcaaga tatttcttga atcaggcgcc ttagaccgct cggccaaaca
2221 accaattact tgttgagaaa tagagtataa ttatcctata aatataacgt ttttgaacac
2281 acatgaacaa ggaagtacag gacaattgat tttgaagaga atgtggattt tgatgtaatt
2341 gttgggattc catttttaat aaggcaataa tattaggtat gtagatatac tagaagttct
2401 cctcgaccgg tcgatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc
2461 atcaggaaat tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca
2521 gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga
2581 ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg
2641 actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat
2701 caccctaatc aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag
2761 ggagcccccg atttagagct tgacggggaa agccggcgaa cgtggcgaga aaggaaggga
2821 agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa
2881 ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc gtccattcgc cattcaggct
2941 gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa
3001 agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg
3061 ttgtaaaacg acggccagtg agcgcgcgta atacgactca ctatagggcg aattgggtac
3121 cgggcccccc ctcgaggtcg acggtatcga taagcttgat atcgaattca gtctactagt
3181 cagttcgagt ttatcattat caatactgcc atttcaaaga atacgtaaat aattaatagt
3241 agtgattttc ctaactttat ttagtcaaaa aattagcctt ttaattctgc tgtaacccgt
3301 acatgcccaa aatagggggc gggttacaca gaatatataa catcgtaggt gtctgggtga
3361 acagtttatt cctggcatcc actaaatata atggagcccg ctttttaagc tggcatccag
3421 aaaaaaaaag aatcccagca ccaaaatatt gttttcttca ccaaccatca gttcataggt
3481 ccattctctt agcgcaacta cagagaacag gggcacaaac aggcaaaaaa cgggcacaac
3541 ctcaatggag tgatgcaacc tgcctggagt aaatgatgac acaaggcaat tgacccacgc
3601 atgtatctat ctcattttct tacaccttct attaccttct gctctctctg atttggaaaa
3661 agctgaaaaa aaaggttgaa accagttccc tgaaattatt cccctacttg actaataagt
3721 atataaagac ggtaggtatt gattgtaatt ctgtaaatct atttcttaaa cttcttaaat
3781 tctactttta tagttagtct tttttttagt tttaaaacac caagaactta gtttcgaata
3841 aacacacata aacaaacaaa agatctgata tggatcgaat tagatctcgc caccatggac
3901 aagaagtatt ctatcggact ggacatcggg actaatagcg tcgggtgggc cgtgatcact
3961 gacgagtaca aggtgccctc taagaagttc aaggtgctcg ggaacaccga ccggcattcc
4021 atcaagaaaa atctgatcgg agctctcctc tttgattcag gggagaccgc tgaagcaacc
4081 cgcctcaagc ggactgctag acggcggtac accaggagga agaaccggat ttgttacctt
4141 caagagatat tctccaacga aatggcaaag gtcgacgaca gcttcttcca taggctggaa
4201 gaatcattcc tcgtggaaga ggataagaag catgaacggc atcccatctt cggtaatatc
4261 gtcgacgagg tggcctatca cgagaaatac ccaaccatct accatcttcg caaaaagctg
4321 gtggactcaa ccgacaaggc agacctccgg cttatctacc tggccctggc ccacatgatc
4381 aagttcagag gccacttcct gatcgagggc gacctcaatc ctgacaatag cgatgtggat
4441 aaactgttca tccagctggt gcagacttac aaccagctct ttgaagagaa ccccatcaat
4501 gcaagcggag tcgatgccaa ggccattctg tcagcccggc tgtcaaagag ccgcagactt
4561 gagaatctta tcgctcagct gccgggtgaa aagaaaaatg gactgttcgg gaacctgatt
4621 gctctttcac ttgggctgac tcccaatttc aagtctaatt tcgacctggc agaggatgcc
4681 aagctgcaac tgtccaagga cacctatgat gacgatctcg acaacctcct ggcccagatc
4741 ggtgaccaat acgccgacct tttccttgct gctaagaatc tttctgacgc catcctgctg
4801 tctgacattc tccgcgtgaa cactgaaatc accaaggccc ctctttcagc ttcaatgatt
4861 aagcggtatg atgagcacca ccaggacctg accctgctta aggcactcgt ccggcagcag
4921 cttccggaga agtacaagga aatcttcttt gaccagtcaa agaatggata cgccggctac
4981 atcgacggag gtgcctccca agaggaattt tataagttta tcaaacctat ccttgagaag
5041 atggacggca ccgaagagct cctcgtgaaa ctgaatcggg aggatctgct gcggaagcag
5101 cgcactttcg acaatgggag cattccccac cagatccatc ttggggagct tcacgccatc
5161 cttcggcgcc aagaggactt ctaccccttt cttaaggaca acagggagaa gattgagaaa
5221 attctcactt tccgcatccc ctactacgtg ggacccctcg ccagaggaaa tagccggttt
5281 gcttggatga ccagaaagtc agaagaaact atcactccct ggaacttcga agaggtggtg
5341 gacaagggag ccagcgctca gtcattcatc gaacggatga ctaacttcga taagaacctc
5401 cccaatgaga aggtcctgcc gaaacattcc ctgctctacg agtactttac cgtgtacaac
5461 gagctgacca aggtgaaata tgtcaccgaa gggatgagga agcccgcatt cctgtcaggc
5521 gaacaaaaga aggcaattgt ggaccttctg ttcaagacca atagaaaggt gaccgtgaag
5581 cagctgaagg aggactattt caagaaaatt gaatgcttcg actctgtgga gattagcggg
5641 gtcgaagatc ggttcaacgc aagcctgggt acctaccatg atctgcttaa gatcatcaag
5701 gacaaggatt ttctggacaa tgaggagaac gaggacatcc ttgaggacat tgtcctgact
5761 ctcactctgt tcgaggaccg ggaaatgatc gaggagaggc ttaagaccta cgcccatctg
5821 ttcgacgata aagtgatgaa gcaacttaaa cggagaagat ataccggatg gggacgcctt
5881 agccgcaaac tcatcaacgg aatccgggac aaacagagcg gaaagaccat tcttgatttc
5941 cttaagagcg acggattcgc taatcgcaac ttcatgcaac ttatccatga tgattccctg
6001 acctttaagg aggacatcca gaaggcccaa gtgtctggac aaggtgactc actgcacgag
6061 catatcgcaa atctggctgg ttcacccgct attaagaagg gtattctcca gaccgtgaaa
6121 gtcgtggacg agctggtcaa ggtgatgggt cgccataaac cagagaacat tgtcatcgag
6181 atggccaggg aaaaccagac tacccagaag ggacagaaga acagcaggga gcggatgaaa
6241 agaattgagg aagggattaa ggagctcggg tcacagatcc ttaaagagca cccggtggaa
6301 aacacccagc ttcagaatga gaagctctat ctgtactacc ttcaaaatgg acgcgatatg
6361 tatgtggacc aagagcttga tatcaacagg ctctcagact acgacgtgga ccacatcgtc
6421 cctcagagct tcctcaaaga cgactcaatt gacaataagg tgctgactcg ctcagacaag
6481 aaccggggaa agtcagataa cgtgccctca gaggaagtcg tgaaaaagat gaagaactat
6541 tggcgccagc ttctgaacgc aaagctgatc actcagcgga agttcgacaa tctcactaag
6601 gctgagaggg gcggactgag cgaactggac aaagcaggat tcattaaacg gcaacttgtg
6661 gagactcggc agattactaa acatgtcgcc caaatccttg actcacgcat gaataccaag
6721 tacgacgaaa acgacaaact tatccgcgag gtgaaggtga ttaccctgaa gtccaagctg
6781 gtcagcgatt tcagaaagga ctttcaattc tacaaagtgc gggagatcaa taactatcat
6841 catgctcatg acgcatatct gaatgccgtg gtgggaaccg ccctgatcaa gaagtaccca
6901 aagctggaaa gcgagttcgt gtacggagac tacaaggtct acgacgtgcg caagatgatt
6961 gccaaatctg agcaggagat cggaaaggcc accgcaaagt acttcttcta cagcaacatc
7021 atgaatttct tcaagaccga aatcaccctt gcaaacggtg agatccggaa gaggccgctc
7081 atcgagacta atggggagac tggcgaaatc gtgtgggaca agggcagaga tttcgctacc
7141 gtgcgcaaag tgctttctat gcctcaagtg aacatcgtga agaaaaccga ggtgcaaacc
7201 ggaggctttt ctaaggaatc aatcctcccc aagcgcaact ccgacaagct cattgcaagg
7261 aagaaggatt gggaccctaa gaagtacggc ggattcgatt caccaactgt ggcttattct
7321 gtcctggtcg tggctaaggt ggaaaaagga aagtctaaga agctcaagag cgtgaaggaa
7381 ctgctgggta tcaccattat ggagcgcagc tccttcgaga agaacccaat tgactttctc
7441 gaagccaaag gttacaagga agtcaagaag gaccttatca tcaagctccc aaagtatagc
7501 ctgttcgaac tggagaatgg gcggaagcgg atgctcgcct ccgctggcga acttcagaag
7561 ggtaatgagc tggctctccc ctccaagtac gtgaatttcc tctaccttgc aagccattac
7621 gagaagctga aggggagccc cgaggacaac gagcaaaagc aactgtttgt ggagcagcat
7681 aagcattatc tggacgagat cattgagcag atttccgagt tttctaaacg cgtcattctc
7741 gctgatgcca acctcgataa agtccttagc gcatacaata agcacagaga caaaccaatt
7801 cgggagcagg ctgagaatat catccacctg ttcaccctca ccaatcttgg tgcccctgcc
7861 gcattcaagt acttcgacac caccatcgac cggaaacgct atacctccac caaagaagtg
7921 ctggacgcca ccctcatcca ccagagcatc accggacttt acgaaactcg gattgacctc
7981 tcacagctcg gaggggatga gggagctccc aagaaaaagc gcaaggtagg tagttccgga
8041 tcctagactc tcgaggcgaa tttcttatga tttatgattt ttattattaa ataagttata
8101 aaaaaaataa gtgtatacaa attttaaagt gactcttagg ttttaaaacg aaaattctta
8161 ttcttgagta actctttcct gtaggtcagg ttgctttctc aggtatagca tgaggtcgct
8221 cttattgacc acacctctac cggcatgccg agcaaatgcc tgcaaatcgc tccccatttc
8281 tctagagcgg ccgccaccgc ggtggagctc cagcttttgt tccctttagt gagggttaat
8341 tgcgcgcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac
8401 aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt
8461 gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc
8521 gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg
8581 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
8641 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
8701 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
8761 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
8821 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
8881 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
8941 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
9001 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
9061 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
9121 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
9181 gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt
9241 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
9301 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
9361 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
9421 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
9481 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag
9541 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
9601 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc
9661 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc
9721 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
9781 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac
9841 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
9901 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc
9961 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact
10021 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
10081 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat
10141 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc
10201 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac
10261 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
10321 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
10381 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg
10441 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg
10501 aaaagtgcca cctgaacgaa gcatctgtgc ttcattttgt agaacaaaaa tgcaacgcga
10561 gagcgctaat ttttcaaaca aagaatctga gctgcatttt tacagaacag aaatgcaacg
10621 cgaaagcgct attttaccaa cgaagaatct gtgcttcatt tttgtaaaac aaaaatgcaa
10681 cgcgagagcg ctaatttttc aaacaaagaa tctgagctgc atttttacag aacagaaatg
10741 caacgcgaga gcgctatttt accaacaaag aatctatact tcttttttgt tctacaaaaa
10801 tgcatcccga gagcgctatt tttctaacaa agcatcttag attacttttt ttctcctttg
10861 tgcgctctat aatgcagtct cttgataact ttttgcactg taggtccgtt aaggttagaa
10921 gaaggctact ttggtgtcta ttttctcttc cataaaaaaa gcctgactcc acttcccgcg
10981 tttactgatt actagcgaag ctgcgggtgc attttttcaa gataaaggca tccccgatta
11041 tattctatac cgatgtggat tgcgcatact ttgtgaacag aaagtgatag cgttgatgat
11101 tcttcattgg tcagaaaatt atgaacggtt tcttctattt tgtctctata tactacgtat
11161 aggaaatgtt tacattttcg tattgttttc gattcactct atgaatagtt cttactacaa
11221 tttttttgtc taaagagtaa tactagagat aaacataaaa aatgtagagg tcgagtttag
11281 atgcaagttc aaggagcgaa aggtggatgg gtaggttata tagggatata gcacagagat
11341 atatagcaaa gagatacttt tgagcaatgt ttgtggaagc ggtattcgca atattttagt
11401 agctcgttac agtccggtgc gtttttggtt ttttgaaagt gcgtcttcag agcgcttttg
11461 gttttcaaaa gcgctctgaa gttcctatac tttctagcta gagaatagga acttcggaat
11521 aggaacttca aagcgtttcc gaaaacgagc gcttccgaaa atgcaacgcg agctgcgcac
11581 atacagctca ctgttcacgt cgcacctata tctgcgtgtt gcctgtatat atatatacat
11641 gagaagaacg gcatagtgcg tgtttatgct taaatgcgta cttatatgcg tctatttatg
11701 taggatgaaa ggtagtctag tacctcctgt gatattatcc cattccatgc ggggtatcgt
11761 atgcttcctt cagcactacc ctttagctgt tctatatgct gccactcctc aattggatta
11821 gtctcatcct tcaatgctat catttccttt gatattggat catctaagaa accattatta
11881 tcatgacatt aacctataaa aataggcgta tcacgaggcc ctttcgtc
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