LOCUS Exported 11921 bp ds-DNA circular SYN 29-AUG-2017
DEFINITION synthetic circular DNA
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 11921)
TITLE Direct Submission
JOURNAL Exported Aug 29, 2017 from MiaoLingPlasmid
http://www.miaolingbio.com
FEATURES Location/Qualifiers
source 1..11921
/organism="synthetic DNA construct"
/mol_type="other DNA"
misc_feature 414..643
/label=Cen1-1
misc_feature 655..855
/label=cyc1t
CDS complement(859..879)
/codon_start=1
/product="nuclear localization signal of SV40 large T
antigen"
/label=SV40 NLS
/translation="PKKKRKV"
CDS complement(892..4992)
/codon_start=1
/product="Cas9 (Csn1) endonuclease from the Streptococcus
pyogenes Type II CRISPR/Cas system"
/label=Cas9
/note="generates RNA-guided double strand breaks in DNA"
/translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
ATLIHQSITGLYETRIDLSQLGGD"
misc_feature complement(4993..5523)
/label=TEFin
misc_feature 5530..6030
/label=mig1t
misc_feature 6031..6098
/label=HDV
misc_RNA complement(6099..6178)
/label=gRNA scaffold
/note="guide RNA scaffold for the Streptococcus pyogenes
CRISPR/Cas9 system"
misc_feature 6179..6198
/label=trp1.gRNA
misc_feature 6199..6235
/label=HH
misc_feature complement(6242..6772)
/label=TEFin
rep_origin complement(7205..7793)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(7964..8824)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(8825..8929)
/gene="bla"
/label=AmpR promoter
misc_feature 9165..11903
/label=LEU2
ORIGIN
1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt cgagctcggt acccgctttt
421 cgtagataat ggaatacaaa tggatatcca gagtatacac atggatagta tacactgaca
481 cgacaattct gtatctcttt atgttaacta ctgtgaggcg ttaaatagag cttgatatat
541 aaaatgttac atttcacagt ctgaactttt gcagattacc taatttggta agatattaat
601 tatgaactga aagttgatgg catccctaaa tttgatgaaa gggggatcac gcgtctgtac
661 agaaaaaaaa gaaaaatttg aaatataaat aacgttctta atactaacat aactataaaa
721 aaataaatag ggacctagac ttcaggttgt ctaactcctt ccttttcggt tagagcggat
781 gtggggggag ggcgtgaatg taagcgtgac ataactaatt acatgactcg aggtccaatg
841 actcgaggtc caaaatcaca ccttcctctt cttcttgggg tcagccctgc tgtctccacc
901 gagctgagag aggtcgattc ttgtttcata gagccccgta attgactgat gaatcagtgt
961 ggcgtccagg acctcctttg tagaggtgta ccgctttctg tctatggtgg tgtcgaagta
1021 cttgaaggct gcaggcgcgc ccaagttggt cagagtaaac aagtggataa tgttttctgc
1081 ctgctccctg atgggcttat ccctgtgctt attgtaagca gaaagcacct tatcgaggtt
1141 agcgtcggcg aggatcactc ttttggagaa ttcgcttatt tgctcgatga tctcatcaag
1201 gtagtgtttg tgttgttcca cgaacagctg cttctgctca ttatcttcgg gagacccttt
1261 gagcttttca tagtggctgg ccagatacaa gaaattaacg tatttagagg gcagtgccag
1321 ctcgttacct ttctgcagct cgcccgcact agcgagcatt cgtttccggc cgttttcaag
1381 ctcaaagaga gagtacttgg gaagcttaat gatgaggtct tttttgacct ctttatatcc
1441 tttcgcctcg agaaagtcga tggggttttt ttcgaagctt gatcgctcca tgattgtgat
1501 gcccagcagt tccttgacgc ttttgagttt tttagacttc cctttctcca ctttggccac
1561 aaccagtaca ctgtaagcga ctgtaggaga atcgaatccg ccgtatttct tggggtccca
1621 atcttttttg cgtgcgatca gcttgtcgct gttccttttc gggaggatac tttccttgga
1681 gaagcctccg gtctgtactt cggtcttttt aacgatgttc acctgcggca tggacaggac
1741 cttccggact gtcgcgaaat ccctaccctt gtcccacacg atttctcctg tttctccgtt
1801 tgtttcgata agtggtcgct tccgaatctc tccattggcc agtgtaatct cggtcttgaa
1861 aaaattcata atattgctgt aaaagaagta cttagcggtg gccttgccta tttcctgctc
1921 agactttgcg atcattttcc taacatcgta cactttatag tctccgtaaa caaattcaga
1981 ttcaagcttg ggatattttt tgataagtgc agtgcctacc actgcattca ggtaggcatc
2041 atgcgcatgg tggtaattgt tgatctctct caccttataa aactgaaagt cctttctgaa
2101 atctgagacc agcttagact tcagagtaat aactttcacc tctcgaatca gtttgtcatt
2161 ttcatcgtac ttggtgttca tgcgtgaatc gagaatttgg gccacgtgct tggtgatctg
2221 gcgtgtctca acaagctgcc ttttgatgaa gccggcttta tccaactcag acaggccacc
2281 tcgttcagcc ttagtcagat tatcgaactt ccgttgtgtg atcagtttgg cgttcagcag
2341 ctgccgccaa taatttttca ttttcttgac aacttcttct gaggggacgt tatcactctt
2401 ccctctattt ttatcggatc ttgtcaacac tttattatca atagaatcat ctttgagaaa
2461 agactggggc acgatatgat ccacgtcgta gtcggagagc cgattgatgt ccagttcctg
2521 atccacgtac atgtccctgc cgttctgcag gtagtacagg tagagcttct cattctgaag
2581 ctgggtgttt tcaactgggt gttccttaag gatttgggac cccagttctt ttataccctc
2641 ttcaatcctc ttcatccttt ccctactgtt cttctgtccc ttctgggtag tttggttctc
2701 tcgggccatc tcgataacga tattctcggg cttatgcctt cccattactt tgacgagttc
2761 atccacgacc ttaacggtct gcagtattcc ctttttgata gctgggctac ctgcaagatt
2821 agcgatgtgc tcgtgaagac tgtccccctg gccagaaact tgtgctttct ggatgtcctc
2881 cttaaaggtg agagagtcat catggatcaa ctgcatgaag ttccggttgg caaatccatc
2941 ggacttaaga aaatccagga ttgtctttcc actctgcttg tctcggatcc cattgatcag
3001 ttttcttgac agccgccccc atcctgtata tcggcgcctc ttgagctgtt tcatgacttt
3061 gtcgtcgaag agatgagcgt aagttttcaa gcgttcttca atcatctccc tatcttcaaa
3121 caacgtaagg gtgaggacaa tgtcctcaag aatgtcctcg ttctcctcat tgtccaggaa
3181 gtccttgtct ttaatgattt tcaggagatc gtgatacgtt cccagggatg cgttgaagcg
3241 atcctccact ccgctgattt caacagagtc gaaacattca atctttttga aatagtcttc
3301 tttgagctgt ttcacggtaa ctttccggtt cgtcttgaag aggaggtcca cgatagcttt
3361 cttctgctct ccagacagga atgctggctt tctcatccct tctgtgacgt atttgacctt
3421 ggtgagctcg ttataaactg tgaagtactc gtacagcaga gagtgtttag gaagcacctt
3481 ttcgttaggc agatttttat caaagttagt catcctttcg atgaaggact gggcagaggc
3541 ccccttatcc acgacttcct cgaagttcca gggagtgatg gtctcttctg atttgcgagt
3601 catccacgcg aatctggaat ttccccgggc gagggggcct acatagtagg gtatccgaaa
3661 tgtgaggatt ttctcaatct tttccctgtt atctttcaaa aaggggtaga aatcctcttg
3721 ccgcctgagg atagcgtgca gttcgcccag gtgaatctgg tgggggatgc ttccattgtc
3781 gaaagtgcgc tgtttgcgca acagatcttc tctgttaagc tttaccagca gctcctcggt
3841 gccgtccatt ttttccaaga tgggcttaat aaatttgtaa aattcctcct ggcttgctcc
3901 gccgtcaatg tatccggcgt agccattttt agactgatcg aagaaaattt ccttgtactt
3961 ctcaggcagt tgctgtctga caagggcctt cagcaaagtc aagtcttggt ggtgctcatc
4021 atagcgcttg atcatactag cgctcagcgg agctttggtg atctccgtgt tcactcgcag
4081 aatatcactc agcagaatgg cgtctgacag gttctttgcc gccaaaaaaa ggtctgcgta
4141 ctggtcgccg atctgggcca gcagattgtc gagatcatca tcgtaggtgt ctttgctcag
4201 ttgaagcttg gcatcttcgg ccaggtcgaa gttagattta aagttggggg tcagcccgag
4261 tgacagggcg ataagattac caaacaggcc gttcttcttc tccccaggga gctgtgcgat
4321 gaggttttcg agccgccggg atttggacag cctagcgctc aggattgctt tggcgtcaac
4381 tccggatgcg ttgatcgggt tctcttcgaa aagctgattg taagtctgaa ccagttggat
4441 aaagagtttg tcaacatcgc tgttgtctgg gttcaggtcc ccctcgatga ggaagtgtcc
4501 ccgaaatttg atcatatgcg ccagcgcgag atagatcaac cgcaagtcag ccttatcagt
4561 actgtctaca agcttcttcc tcagatgata tatggttggg tacttttcat ggtacgccac
4621 ctcgtccacg atattgccaa agattgggtg gcgctcgtgc tttttatcct cctccaccaa
4681 aaaggactcc tccagcctat ggaagaaaga gtcatccacc ttagccatct cattactaaa
4741 gatctcctgc aggtagcaga tccgattctt tctgcgggta tatctgcgcc gtgctgttct
4801 tttgagccgc gtggcttcgg ccgtctcccc ggagtcgaac aggagggcgc caatgaggtt
4861 cttctttatg ctgtggcgat cggtattgcc cagaactttg aattttttgc tcggcacctt
4921 gtactcgtcc gtaatgacgg cccagccgac gctgtttgtg ccgatatcga gcccaatgga
4981 gtacttcttg tcctgcggtt agtactgcaa aaagtgctgg tcggatgacg tggcgtcttg
5041 tgtcgatggg aatgggacca cacccggccg tgtgctcaaa gcccgtggca attgctgctg
5101 cctctgaaac tcaccatttt gaatgattct tatactcaga aggaaatgct taacgatttc
5161 gggtgtgagt tgacaaggag agagagaaaa gaagaggaaa ggtaattcgg ggacggtggt
5221 cttttatacc cttggctaaa gtcccaacca caaagcaaaa aaattttcag tagtctattt
5281 tgcgtccggc atgggttacc cggatggcca gacaaagaaa ctagtacaaa gtctgaacaa
5341 gcgtagattc cagactgcag taccctacgc ccttaacggc aagtgtggga accgggggag
5401 gtttgatatg tggggtgaag ggggctctcg ccggggttgg gcccgctact gggtcaattt
5461 ggggtcaatt ggggcaattg gggctgtttt ttgggacaca aatacgccgc caacccggtc
5521 tctgtcgaca aacccaaaag ggccgaaggc tgggccgagg ctctgagatg gatgagatag
5581 ctggggaaaa caggtgagta aatgattagt aatcagacac ccaggcagat ccgatacacc
5641 ggtatgattg catcacgtga cttcattgct cctccacaga gagattagtc atgctctttt
5701 tcagaatcgc aaacccttcc cccacttact taacccctca agctcaactg aaacagtcac
5761 tcactgtaga tgtgctccga aggcccagga acaatgtggg gtaggaaaat attatgtcta
5821 tacgagacga atacggtcac cgactctaat gtaagtgata ccatgtatag tagtgtcttc
5881 tggtacagaa ctccaagtaa tgtgtactta atcccctgta atacaatacc gtgctctcat
5941 tctcttacgg tatcattaca ttcatctata tacagccaat gggcaatgcg tgtgctgagc
6001 tcagcacgtt aaattatcga ccggccagtg gtcccattcg ccatgccgaa gcatgttgcc
6061 cagccggcgc cagcgaggag gctgggacca tgccggccaa aagcaccgac tcggtgccac
6121 tttttcaagt tgataacgga ctagccttat tttaacttgc tatttctagc tctaaaaccg
6181 atggcgtcct gatccagtga cgagcttact cgtttcgtcc tcacggactc atcagactgg
6241 actgcggtta gtactgcaaa aagtgctggt cggatgacgt ggcgtcttgt gtcgatggga
6301 atgggaccac acccggccgt gtgctcaaag cccgtggcaa ttgctgctgc ctctgaaact
6361 caccattttg aatgattctt atactcagaa ggaaatgctt aacgatttcg ggtgtgagtt
6421 gacaaggaga gagagaaaag aagaggaaag gtaattcggg gacggtggtc ttttataccc
6481 ttggctaaag tcccaaccac aaagcaaaaa aattttcagt agtctatttt gcgtccggca
6541 tgggttaccc ggatggccag acaaagaaac tagtacaaag tctgaacaag cgtagattcc
6601 agactgcagt accctacgcc cttaacggca agtgtgggaa ccgggggagg tttgatatgt
6661 ggggtgaagg gggctctcgc cggggttggg cccgctactg ggtcaatttg gggtcaattg
6721 gggcaattgg ggctgttttt tgggacacaa atacgccgcc aacccggtct ctgtttaaac
6781 aaacaagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca
6841 caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag
6901 tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt
6961 cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc
7021 gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg
7081 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa
7141 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
7201 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
7261 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
7321 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
7381 gaagcgtggc gctttctcaa tgctcacgct gtaggtatct cagttcggtg taggtcgttc
7441 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
7501 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
7561 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
7621 ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag
7681 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
7741 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
7801 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
7861 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt
7921 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca
7981 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg
8041 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac
8101 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg
8161 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc
8221 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta
8281 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac
8341 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc
8401 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac
8461 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact
8521 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa
8581 tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt
8641 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca
8701 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa
8761 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac
8821 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg
8881 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc
8941 gaaaagtgcc acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata
9001 ggcgtatcac gaggcccaga tcctctagag tcgaagcggc cgctatgtct gataaaagga
9061 tgtaacatag gcaagctgct cgtgagtgtt gagtacgaac cttagatcca aatcacccgc
9121 acccacggat atacttgctt gaatatacag tagtatgcgg ccgcttcgac accatatcat
9181 ataaaactaa caatgcattg cttattacga agactacccg ttgctatctc cacaccgtta
9241 tctccacggt ccaaaggctg ctcaatgtgc tgcatacgta acgtggggtg caaccttgag
9301 cacatagtac ttttccgaaa accggcgata attaagtgtg cactccaact tttcacactg
9361 agcgtaaaat gtggagaaga aatcggcact aaaaagtcag gtagactgga aaatgcgcca
9421 tgaaatgaat atctcttgct acagtaatgc ccagcatcga ggggtattgt gtcaccaaca
9481 ctatagtggc agctgaagcg ctcgtgattg tagtatgagt ctttattggt gatgggaaga
9541 gttcactcaa tattctcgtt actgccaaaa caccacggta atcggccaga caccatggat
9601 gtagatcacc aagcctgtga atgttattcg agctaaaatg cacatggttg gtgaaaggag
9661 tagttgctgt cgaattccgt cgtcgcctga gtcatcattt atttaccagt tggccacaaa
9721 cccttgacga tctcgtatgt cccctccgac atactcccgg ccggctgggg tacgttcgat
9781 agcgctatcg gcatcgacaa ggtttgggtc cctagccgat accgcactac ctgagtcaca
9841 atcttcggag gtttagtctt ccacatagca cgggcaaaag tgcgtatata tacaagagcg
9901 tttgccagcc acagattttc actccacaca ccacatcaca catacaacca cacacatcca
9961 caatggaacc cgaaactaag aagaccaaga ctgactccaa gaagattgtt cttctcggcg
10021 gcgacttctg tggccccgag gtgattgccg aggccgtcaa ggtgctcaag tctgttgctg
10081 aggcctccgg caccgagttt gtgtttgagg accgactcat tggaggagct gccattgaga
10141 aggagggcga gcccatcacc gacgctactc tcgacatctg ccgaaaggct gactctatta
10201 tgctcggtgc tgtcggaggc gctgccaaca ccgtatggac cactcccgac ggacgaaccg
10261 acgtgcgacc cgagcagggt ctcctcaagc tgcgaaagga cctgaacctg tacgccaacc
10321 tgcgaccctg ccagctgctg tcgcccaagc tcgccgatct ctcccccatc cgaaacgttg
10381 agggcaccga cttcatcatt gtccgagagc tcgtcggagg tatctacttt ggagagcgaa
10441 aggaggatga cggatctggc gtcgcttccg acaccgagac ctactccgtt cctgaggttg
10501 agcgaattgc ccgaatggcc gccttcctgg cccttcagca caacccccct cttcccgtgt
10561 ggtctcttga caaggccaac gtgctggcct cctctcgact ttggcgaaag actgtcactc
10621 gagtcctcaa ggacgaattc ccccagctcg agctcaacca ccagctgatc gactcggccg
10681 ccatgatcct catcaagcag ccctccaaga tgaatggtat catcatcacc accaacatgt
10741 ttggcgatat catctccgac gaggcctccg tcatccccgg ttctctgggt ctgctgccct
10801 ccgcctctct ggcttctctg cccgacacca acgaggcgtt cggtctgtac gagccctgtc
10861 acggatctgc ccccgatctc ggcaagcaga aggtcaaccc cattgccacc attctgtctg
10921 ccgccatgat gctcaagttc tctcttaaca tgaagcccgc cggtgacgct gttgaggctg
10981 ccgtcaagga gtccgtcgag gctggtatca ctaccgccga tatcggaggc tcttcctcca
11041 cctccgaggt cggagacttg ttgccaacaa ggtcaaggag ctgctcaaga aggagtaagt
11101 cgtttctacg acgcattgat ggaaggagca aactgacgcg cctgcgggtt ggtctaccgg
11161 cagggtccgc tagtgtataa gactctataa aaagggccct gccctgctaa tgaaatgatg
11221 atttataatt taccggtgta gcaaccttga ctagaagaag cagattgggt gtgtttgtag
11281 tggaggacag tggtacgttt tggaaacagt cttcttgaaa gtgtcttgtc tacagtatat
11341 tcactcataa cctcaatagc caagggtgta gtcggtttat taaaggaagg gagttgtggc
11401 tgatgtggat agatatcttt aagctggcga ctgcacccaa cgagtgtggt ggtagcttgt
11461 tactgtatat tcggtaagat atattttgtg gggttttagt ggtgtttggt aggttagtgc
11521 ttggtatatg agttgtaggc atgacaattt ggaaaggggt ggactttggg aatattgtgg
11581 gatttcaata ccttagtttg tacagggtaa ttgttacaaa tgatacaaag aactgtattt
11641 cttttcattt gttttaattg gttgtatatc aagtccgtta gacgagctca gtgccatggc
11701 ttttggcact gtatttcatt tttagaggta cactacattc agtgaggtat ggtaaggttg
11761 agggcataat gaaggcacct tgtactgaca gtcacagacc tctcaccgag aattttatga
11821 gatatactcg ggttcatttt aggctccgat tcgattcaaa ttattactgt cgaaatcggt
11881 tgagcatccg ttgatttccg aacagatctg gccctttcgt c
//