LOCUS Exported 7202 bp ds-DNA circular SYN 08-九月-2017
KEYWORDS pMA5
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7202)
TITLE Direct Submission
http://www.miaolingbio.com
FEATURES Location/Qualifiers
source 1..7202
/organism="synthetic DNA construct"
/mol_type="other DNA"
rep_origin 429..884
/direction=RIGHT
/note="f1 ori"
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 1006..1110
/gene="bla"
/note="AmpR promoter"
CDS 1111..1971
/codon_start=1
/note="Amp"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 2142..2733
/direction=RIGHT
/note="ori"
/note="high-copy-number colE1/pMB1/pBR322/pUC origin of
replication"
misc_feature 5317..6078
/note="Kan"
misc_feature 6934..3
/note="HpaII promoter"
ORIGIN
1 catatgagtt atgcagtttg tagaatgcaa aaagtgaaat cagggggatc ctctagagtc
61 gagctcaagc tagcttggta cgtaccagat ctgagatcac gcgttctaga ggtcgaaatt
121 cacctcgaaa gcaagctgat aaaccgatac aattaaaggc tccttttgga gccttttttt
181 ttggagattt tcaacgtgaa aaaattatta ttcgcaattc caagctaatt cacctcgaaa
241 gcaagctgat aaaccgatac aattaaaggc tccttttgga gccttttttt ttggagattt
301 tcaacgtgaa aaaattatta ttcgcaattc caagctctgc ctcgcgcgtt tcggtgatga
361 cggtgaaaac ctctgacaca tgcagctccc ggagacggtc acagcttgtc tgtaagcgga
421 tgcagatcac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag
481 cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt
541 tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt
601 ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg
661 tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt
721 taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt
781 tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca
841 aaaatttaac gcgaatttta acaaaatatt aacgcttaca atttgatctg cgctcggtcg
901 ttcggctgcg gcgagcggta tcagctcact caaagcggta atacggttat cccagaatcc
961 ggggataacg caggaaagac aggtggcact tttcggggaa atgtgcgcgg aacccctatt
1021 tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa
1081 atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt
1141 attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa
1201 gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac
1261 agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt
1321 aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt
1381 cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat
1441 cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac
1501 actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg
1561 cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc
1621 ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa
1681 ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag
1741 gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct
1801 gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat
1861 ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa
1921 cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac
1981 caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc
2041 taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc
2101 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg
2161 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg
2221 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca
2281 aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg
2341 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg
2401 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga
2461 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga agtactgaga
2521 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
2581 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
2641 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
2701 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
2761 ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct
2821 gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc
2881 gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc
2941 cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg
3001 ggcagtgagc gcaacgcaat taatgtgagt tagctcactc attaggcacc ccaggcttta
3061 cactttatgc ttccggcata ttctcaataa accctttagg gaaataggcc aggttttcac
3121 cgtaacacgc cacatcttgc gaatatatgt gtagaaactg ccggaaatcg tcgtggtatt
3181 cactccagag cgatgaaaac gtttcagttt gctcatggaa aacggtgtaa caagggtgaa
3241 cactatccca tatcaccagc tcaccgtctt tcattgccat acgaaattcc ggatgagcat
3301 tcatcaggcg ggcaagaatg tgaataaagg ccggataaaa cttgtgctta tttttcttta
3361 cggtctttaa aaaggccgta atatccagct aaacggtctg gttataggta cattgagcaa
3421 ctgactgaaa tgcctcaaaa tgttctttac gatgccattg ggatatatca acggtggtat
3481 atccagtgat ttttttctcc attttagctt ccttagctcc tgaaaatctc gataactcaa
3541 aaaatacgcc cggtagtgat cttatttcat tatggtgaaa gttggaacct cttacgtgcc
3601 gatcaacgtc tcattttcgc caaaagttgg cccagggctt cccggtatca acagggacac
3661 caggatttat ttattctgcg aagtgatctt ccgtcacagg tatttattcg aagacgaaag
3721 ggcatcgcgc gcggggaatt cccgggagag ctcgatatcg catgcggtac ctctagaaga
3781 agcttggaga caaggtaaag gataaaacag cacaattcca agaaaaacac gatttagaac
3841 ctaaaaagaa cgaatttgaa ctaactcata accgagaggt aaaaaaagaa cgaagtcgag
3901 atcagggaat gagtttataa aataaaaaaa gcacctgaaa aggtgtcttt ttttgatggt
3961 tttgaacttg ttctttctta tcttgataca tatagaaata acgtcatttt tattttagtt
4021 gctgaaaggt gcgttgaagt gttggtatgt atgtgtttta aagtattgaa aacccttaaa
4081 attggttgca cagaaaaacc ccatctgtta aagttataag tgactaaaca aataactaaa
4141 tagatggggg tttcttttaa tattatgtgt cctaatagta gcatttattc agatgaaaaa
4201 tcaagggttt tagtggacaa gacaaaaagt ggaaaagtga gaccatggag agaaaagaaa
4261 atcgctaatg ttgattactt tgaacttctg catattcttg aatttaaaaa ggctgaaaga
4321 gtaaaagatt gtgctgaaat attagagtat aaacaaaatc gtgaaacagg cgaaagaaag
4381 ttgtatcgag tgtggttttg taaatccagg ctttgtccaa tgtgcaactg gaggagagca
4441 atgaaacatg gcattcagtc acaaaaggtt gttgctgaag ttattaaaca aaagccaaca
4501 gttcgttggt tgtttctcac attaacagtt aaaaatgttt atgatggcga agaattaaat
4561 aagagtttgt cagatatggc tcaaggattt cgccgaatga tgcaatataa aaaaattaat
4621 aaaaatcttg ttggttttat gcgtgcaacg gaagtgacaa taaataataa agataattct
4681 tataatcagc acatgcatgt attggtatgt gtggaaccaa cttattttaa gaatacagaa
4741 aactacgtga atcaaaaaca atggattcaa ttttggaaaa aggcaatgaa attagactat
4801 gatccaaatg taaaagttca aatgattcga ccgaaaaata aatataaatc ggatatacaa
4861 tcggcaattg acgaaactgc aaaatatcct gtaaaggata cggattttat gaccgatgat
4921 gaagaaaaga atttgaaacg tttgtctgat ttggaggaag gtttacaccg taaaaggtta
4981 atctcctatg gtggtttgtt aaaagaaata cataaaaaat taaaccttga tgacacagaa
5041 gaaggcgatt tgattcatac agatgatgac gaaaaagccg atgaagatgg attttctatt
5101 attgcaatgt ggaattggga acggaaaaat tattttatta aagagtagtt caacaaacgg
5161 gccagtttgt tgaagattag atgctataat tgttattaaa aggattgaag gatgcttagg
5221 aagacgagtt attaatagct gaataagaac ggtgctctcc aaatattctt atttagaaaa
5281 gcaaatctaa aattatctga aaagggaatg agaatagtga atggaccaat aataatgact
5341 agagaagaaa gaatgaagat tgttcatgaa attaaggaac gaatattgga taaatatggg
5401 gatgatgtta aggctattgg tgtttatggc tctcttggtc gtcagactga tgggccctat
5461 tcggatattg agatgatgtg tgtcatgtca acagaggaag cagagttcag ccatgaatgg
5521 acaaccggtg agtggaaggt ggaagtgaat tttgatagcg aagagattct actagattat
5581 gcatctcagg tggaatcaga ttggccgctt acacatggtc aatttttctc tattttgccg
5641 atttatgatt caggtggata cttagagaaa gtgtatcaaa ctgctaaatc ggtagaagcc
5701 caaacgttcc acgatgcgat ttgtgccctt atcgtagaag agctgtttga atatgcaggc
5761 aaatggcgta atattcgtgt gcaaggaccg acaacatttc taccatcctt gactgtacag
5821 gtagcaatgg caggtgccat gttgattggt ctgcatcatc gcatctgtta tacgacgagc
5881 gcttcggtct taactgaagc agttaagcaa tcagatcttc cttcaggtta tgaccatctg
5941 tgccagttcg taatgtctgg tcaactttcc gactctgaga aacttctgga atcgctagag
6001 aatttctgga atgggattca ggagtggaca gaacgacacg gatatatagt ggatgtgtca
6061 aaacgcatac cattttgaac gatgacctct aataattgtt aatcatgttg gttacgtatt
6121 tattaacttc tcctagtatt agtaattatc atggctgtca tggcgcatta acggaataaa
6181 gggtgtgctt aaatcgggcc attttgcgta ataagaaaaa ggattaatta tgagcgaatt
6241 gaattaataa taaggtaata gatttacatt agaaaatgaa aggggatttt atgcgtgaga
6301 atgttacagt ctatcccggc attgccagtc ggggatatta aaaagagtat aggtttttat
6361 tgcgataaac taggtttcac tttggttcac catgaagatg gattcgcagt tctaatgtgt
6421 aatgaggttc ggattcatct atgggaggca agtgatgaag gctggcgctc tcgtagtaat
6481 gattcaccgg tttgtacagg tgcggagtcg tttattgctg gtactgctag ttgccgcatt
6541 gaagtagagg gaattgatga attatatcaa catattaagc ctttgggcat tttgcacccc
6601 aatacatcat taaaagatca gtggtgggat gaacgagact ttgcagtaat tgatcccgac
6661 aacaatttga ttagcttttt tcaacaaata aaaagctaaa atctattatt aatctgttca
6721 gcaatcgggc gcgattgctg aataaaagat acgagagacc tctcttgtat cttttttatt
6781 ttgagtggtt ttgtccgtta cactagaaaa ccgaaagaca ataaaaattt tattcttgct
6841 gagtctggct ttcggtaagc tagacaaaac ggacaaaata aaaattggca agggtttaaa
6901 ggtggagatt ttttgagtga tcttctcaaa aaatactacc tgtcccttgc tgatttttaa
6961 acgagcacga gagcaaaacc cccctttgct gaggtggcag agggcaggtt tttttgtttc
7021 ttttttctcg taaaaaaaag aaaggtctta aaggttttat ggttttggtc ggcactgccg
7081 acagcctcgc agagcacaca ctttatgaat ataaagtata gtgtgttata ctttacttgg
7141 aagtggttgc cggaaagagc gaaaatgcct cacatttgtg ccacctaaaa aggagcgatt
7201 ta
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