LOCUS Exported 7215 bp ds-DNA circular SYN 10-AUG-2017
DEFINITION synthetic circular DNA
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7215)
TITLE Direct Submission
JOURNAL Exported Sep 15, 2017 from MiaoLingPlasmid
http://www.miaolingbio.com
FEATURES Location/Qualifiers
source 1..7215
/organism="synthetic DNA construct"
/mol_type="other DNA"
CDS 4..113
/codon_start=1
/label=Lip A SP
/translation="ILIWSASAAASKRMSMT*ISLLF*FLLNKRKS*HRR"
primer_bind complement(185..209)
/label=PMA0911R
rep_origin 537..992
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 1018..1122
/gene="bla"
/label=AmpR promoter
CDS 1123..1419
/codon_start=1
/label=Amp
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
DCNLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSAE"
CDS 1466..1984
/codon_start=1
/label=Amp
/translation="MTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTR
LDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAE
IGASLIKHW"
rep_origin 2155..2746
/direction=RIGHT
/label=ori
/note="high-copy-number colE1/pMB1/pBR322/pUC origin of
replication"
promoter complement(3516..3618)
/label=cat promoter
/note="promoter of the E. coli cat gene"
CDS 4157..5161
/codon_start=1
/label=rep
/translation="MGVSFNIMCPNSSIYSDEKSRVLVDKTKSGKVRPWREKKIANVDY
FELLHILEFKKAERVKDCAEILEYKQNRETGERKLYRVWFCKSRLCPMCNWRRAMKHGI
QSQKVVAEVIKQKPTVRWLFLTLTVKNVYDGEELNKSLSDMAQGFRRMMQYKKINKNLV
GFMRATEVTINNKDNSYNQHMHVLVCVEPTYFKNTENYVNQKQWIQFWKKAMKLDYDPN
VKVQMIRPKNKYKSDIQSAIDETAKYPVKDTDFMTDDEEKNLKRLSDLEEGLHRKRLIS
YGGLLKEIHKKLNLDDTEEGDLIHTDDDEKADEDGFSIIAMWNWERKNYFIKE"
CDS 5321..6091
/codon_start=1
/label=Kana Neo
/translation="MRIVNGPIIMTREERMKIVHEIKERILDKYGDDVKAIGVYGSLGR
QTDGPYSDIEMMCVMSTEEAEFSHEWTTGEWKVEVNFDSEEILLDYASQVESDWPLTHG
QFFSILPIYDSGGYLEKVYQTAKSVEAQTFHDAICALIVEELFEYAGKWRNIRVQGPTT
FLPSLTVQVAMAGAMLIGLHHRICYTTSASVLTEAVKQSDLPSGYDHLCQFVMSGQLSD
SEKLLESLENFWNGIQEWTERHGYIVDVSKRIPF"
CDS 6314..6712
/codon_start=1
/label=bleomycin
/translation="MLQSIPALPVGDIKKSIGFYCDKLGFTLVHHEDGFAVLMCNEVRI
HLWEASDEGWRSRSNDSPVCTGAESFIAGTASCRIEVEGIDELYQHIKPLGILHPNTSL
KDQWWDERDFAVIDPDNNLISFFQQIKS"
primer_bind 7172..7195
/label=PMA0911F
ORIGIN
1 catatcctca tatggtcagc atccgccgca gcttcgaagc gtatgtcgat gacatgaata
61 tcattactgt tctgattcct gctgaacaaa aggaaatcat gacaccgccg gaattcgtcg
121 acaagcttgg taccagatct gatatccctg cagggatcct ctagagtcga gctcaagcta
181 gcttggtacg taccagatct gagatcccgg gttctagagg tcgaaattca cctcgaaagc
241 aagctgataa accgatacaa ttaaaggctc cttttggagc cttttttttt ggagattttc
301 aacgtgaaaa aattattatt cgcaattcca agctaattca cctcgaaagc aagctgataa
361 accgatacaa ttaaaggctc cttttggagc cttttttttt ggagattttc aacgtgaaaa
421 aattattatt cgcaattcca agctctgcct cgcgcgtttc ggtgatgacg gtgaaaacct
481 ctgacacatg cagctcccgg agacggtcac agcttgtctg taagcggatg cagatcacgc
541 gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac
601 acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt
661 cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc
721 tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc
781 gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact
841 cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg
901 gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc
961 gaattttaac aaaatattaa cgcttacaat ttaggtggca cttttcgggg aaatgtgcgc
1021 ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa
1081 taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc
1141 cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa
1201 acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgac
1261 tgcaatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg
1321 atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa
1381 gagcaactcg gtcgccgcat acactattct gcagaatgac ttggttgagt actcaccagt
1441 cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac
1501 catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct
1561 aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga
1621 gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac
1681 aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat
1741 agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg
1801 ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc
1861 actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc
1921 aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg
1981 gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta
2041 atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg
2101 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga
2161 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
2221 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
2281 agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa
2341 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
2401 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
2461 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
2521 cgaagtactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
2581 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
2641 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
2701 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
2761 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt
2821 atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg
2881 cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg
2941 caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc
3001 cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc
3061 accccaggct ttacacttta tgcttccggc atattctcaa taaacccttt agggaaatag
3121 gccaggtttt caccgtaaca cgccacatct tgcgaatata tgtgtagaaa ctgccggaaa
3181 tcgtcgtggt attcactcca gagcgatgaa aacgtttcag tttgctcatg gaaaacggtg
3241 taacaagggt gaacactatc ccatatcacc agctcaccgt ctttcattgc catacgaaat
3301 tccggatgag cattcatcag gcgggcaaga atgtgaataa aggccggata aaacttgtgc
3361 ttatttttct ttacggtctt taaaaaggcc gtaatatcca gctaaacggt ctggttatag
3421 gtacattgag caactgactg aaatgcctca aaatgttctt tacgatgcca ttgggatata
3481 tcaacggtgg tatatccagt gatttttttc tccattttag cttccttagc tcctgaaaat
3541 ctcgataact caaaaaatac gcccggtagt gatcttattt cattatggtg aaagttggaa
3601 cctcttacgt gccgatcaac gtctcatttt cgccaaaagt tggcccaggg cttcccggta
3661 tcaacaggga caccaggatt tatttattct gcgaagtgat cttccgtcac aggtatttat
3721 tcgaagacga aagggcatcg cgcgcgggaa attcccggga gagctcgata tcgcatgcgg
3781 tacctctaga agaagcttgg agacaaggta aaggataaaa cagcacaatt ccaagaaaaa
3841 cacgatttag aacctaaaaa gaacgaattt gaactaactc ataaccgaga ggtaaaaaaa
3901 gaacgaagtc gagatcaggg aatgagttta taaaataaaa aaagcacctg aaaaggtgtc
3961 tttttttgat ggttttgaac ttgttctttc ttatcttgat acatatagaa ataacgtcat
4021 ttttatttta gttgctgaaa ggtgcgttga agtgttggta tgtatgtgtt ttaaagtatt
4081 gaaaaccctt aaaattggtt gcacagaaaa accccatctg ttaaagttat aagtgactaa
4141 acaaataact aaatagatgg gggtttcttt taatattatg tgtcctaata gtagcattta
4201 ttcagatgaa aaatcaaggg ttttagtgga caagacaaaa agtggaaaag tgagaccatg
4261 gagagaaaag aaaatcgcta atgttgatta ctttgaactt ctgcatattc ttgaatttaa
4321 aaaggctgaa agagtaaaag attgtgctga aatattagag tataaacaaa atcgtgaaac
4381 aggcgaaaga aagttgtatc gagtgtggtt ttgtaaatcc aggctttgtc caatgtgcaa
4441 ctggaggaga gcaatgaaac atggcattca gtcacaaaag gttgttgctg aagttattaa
4501 acaaaagcca acagttcgtt ggttgtttct cacattaaca gttaaaaatg tttatgatgg
4561 cgaagaatta aataagagtt tgtcagatat ggctcaagga tttcgccgaa tgatgcaata
4621 taaaaaaatt aataaaaatc ttgttggttt tatgcgtgca acggaagtga caataaataa
4681 taaagataat tcttataatc agcacatgca tgtattggta tgtgtggaac caacttattt
4741 taagaataca gaaaactacg tgaatcaaaa acaatggatt caattttgga aaaaggcaat
4801 gaaattagac tatgatccaa atgtaaaagt tcaaatgatt cgaccgaaaa ataaatataa
4861 atcggatata caatcggcaa ttgacgaaac tgcaaaatat cctgtaaagg atacggattt
4921 tatgaccgat gatgaagaaa agaatttgaa acgtttgtct gatttggagg aaggtttaca
4981 ccgtaaaagg ttaatctcct atggtggttt gttaaaagaa atacataaaa aattaaacct
5041 tgatgacaca gaagaaggcg atttgattca tacagatgat gacgaaaaag ccgatgaaga
5101 tggattttct attattgcaa tgtggaattg ggaacggaaa aattatttta ttaaagagta
5161 gttcaacaaa cgggccagtt tgttgaagat tagatgctat aattgttatt aaaaggattg
5221 aaggatgctt aggaagacga gttattaata gctgaataag aacggtgctc tccaaatatt
5281 cttatttaga aaagcaaatc taaaattatc tgaaaaggga atgagaatag tgaatggacc
5341 aataataatg actagagaag aaagaatgaa gattgttcat gaaattaagg aacgaatatt
5401 ggataaatat ggggatgatg ttaaggctat tggtgtttat ggctctcttg gtcgtcagac
5461 tgatgggccc tattcggata ttgagatgat gtgtgtcatg tcaacagagg aagcagagtt
5521 cagccatgaa tggacaaccg gtgagtggaa ggtggaagtg aattttgata gcgaagagat
5581 tctactagat tatgcatctc aggtggaatc agattggccg cttacacatg gtcaattttt
5641 ctctattttg ccgatttatg attcaggtgg atacttagag aaagtgtatc aaactgctaa
5701 atcggtagaa gcccaaacgt tccacgatgc gatttgtgcc cttatcgtag aagagctgtt
5761 tgaatatgca ggcaaatggc gtaatattcg tgtgcaagga ccgacaacat ttctaccatc
5821 cttgactgta caggtagcaa tggcaggtgc catgttgatt ggtctgcatc atcgcatctg
5881 ttatacgacg agcgcttcgg tcttaactga agcagttaag caatcagatc ttccttcagg
5941 ttatgaccat ctgtgccagt tcgtaatgtc tggtcaactt tccgactctg agaaacttct
6001 ggaatcgcta gagaatttct ggaatgggat tcaggagtgg acagaacgac acggatatat
6061 agtggatgtg tcaaaacgca taccattttg aacgatgacc tctaataatt gttaatcatg
6121 ttggttacgt atttattaac ttctcctagt attagtaatt atcatggctg tcatggcgca
6181 ttaacggaat aaagggtgtg cttaaatcgg gccattttgc gtaataagaa aaaggattaa
6241 ttatgagcga attgaattaa taataaggta atagatttac attagaaaat gaaaggggat
6301 tttatgcgtg agaatgttac agtctatccc ggcattgcca gtcggggata ttaaaaagag
6361 tataggtttt tattgcgata aactaggttt cactttggtt caccatgaag atggattcgc
6421 agttctaatg tgtaatgagg ttcggattca tctatgggag gcaagtgatg aaggctggcg
6481 ctctcgtagt aatgattcac cggtttgtac aggtgcggag tcgtttattg ctggtactgc
6541 tagttgccgc attgaagtag agggaattga tgaattatat caacatatta agcctttggg
6601 cattttgcac cccaatacat cattaaaaga tcagtggtgg gatgaacgag actttgcagt
6661 aattgatccc gacaacaatt tgattagctt ttttcaacaa ataaaaagct aaaatctatt
6721 attaatctgt tcagcaatcg ggcgcgattg ctgaataaaa gatacgagag acctctcttg
6781 tatctttttt attttgagtg gttttgtccg ttacactaga aaaccgaaag acaataaaaa
6841 ttttattctt gctgagtctg gctttcggta agctagacaa aacggacaaa ataaaaattg
6901 gcaagggttt aaaggtggag attttttgag tgatcttctc aaaaaatact acctgtccct
6961 tgctgatttt taaacgagca cgagagcaaa accccccttt gctgaggtgg cagagggcag
7021 gtttttttgt ttcttttttc tcgtaaaaaa aagaaaggtc ttaaaggttt tatggttttg
7081 gtcggcactg ccgacagcct cgcagagcac acactttatg aatataaagt atagtgtgtt
7141 atactttact tggaagtggt tgccggaaag agcgaaaatg cctcacattt gtgccaccta
7201 aaaaggagcg attta
//