LOCUS Exported File 7956 bp ds-DNA circular SYN 07-2-2015
DEFINITION .
ACCESSION .
VERSION .
KEYWORDS Untitled
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7956)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported 2015-2-7 from MLCC
http://www.miaolingbio.com
FEATURES Location/Qualifiers
source 1..7956
/organism="synthetic DNA construct"
/mol_type="other DNA"
primer_bind 1239..1255
/note="M13 fwd"
/note="common sequencing primer, one of multiple similar
variants"
CDS complement(1298..2380)
/codon_start=1
/gene="lacI"
/product="lac repressor"
/note="lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
/translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
EACKTAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
ALADSLMQLARQVSRLESGQ"
protein_bind 2764..2788
/bound_moiety="lac repressor encoded by lacI"
/note="lac operator"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter 6033..6137
/gene="bla"
/note="AmpR promoter"
CDS 6138..6998
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/note="AmpR"
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 7169..7757
/direction=RIGHT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 ttaagttatt ggtatgactg gttttaagcg caaaaaaagt tgctttttcg tacctattaa
61 tgtatcgttt tagaaaaccg actgtaaaaa gtacagtcgg cattatctca tattataaaa
121 gccagtcatt aggcctatct gacaattcct gaatagagtt cataaacaat cctgcatgat
181 aaccatcaca aacagaatga tgtacctgta aagatagcgg taaatatatt gaattacctt
241 tattaatgaa ttttcctgct gtaataatgg gtagaaggta attactatta ttattgatat
301 ttaagttaaa cccagtaaat gaagtccatg gaataataga aagagaaaaa gcattttcag
361 gtataggtgt tttgggaaac aatttccccg aaccattata tttctctaca tcagaaaggt
421 ataaatcata aaactctttg aagtcattct ttacaggagt ccaaatacca gagaatgttt
481 tagatacacc atcaaaaatt gtataaagtg gctctaactt atcccaataa cctaactctc
541 cgtcgctatt gtaaccagtt ctaaaagctg tatttgagtt tatcaccctt gtcactaaga
601 aaataaatgc agggtaaaat ttatatcctt cttgttttat gtttcggtat aaaacactaa
661 tatcaatttc tgtggttata ctaaaagtcg tttgttggtt caaataatga ttaaatatct
721 cttttctctt ccaattgtct aaatcaattt tattaaagtt catttgatat gcctcctaaa
781 tttttatcta aagtgaattt aggaggctta cttgtctgct ttcttcatta gaatcaatcc
841 ttttttaaaa gtcaatatta ctgtaacata aatatatatt ttaaaaatat cccactttat
901 ccaattttcg tttgttgaac taatgggtgc tttagttgaa gaataaaaga ccacattaaa
961 aaatgtggtc ttttgtgttt ttttaaagga tttgagcgta gcgaaaaatc cttttctttc
1021 ttatcttgat aataagggta actattgccg atcgtccatt ccgacagcat cgccagtcac
1081 tatggcgtgc tgctagcgcc attcgccatt caggctgcgc aactgttggg aagggcgatc
1141 ggtgcgggcc tcttcgctat tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt
1201 aagttgggta acgccagggt tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt
1261 cgagctcagg ccttaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg
1321 aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg
1381 tattgggcgc cagggtggtt tttcttttca ccagtgagac gggcaacagc tgattgccct
1441 tcaccgcctg gccctgagag agttgcagca agcggtccac gctggtttgc cccagcaggc
1501 gaaaatcctg tttgatggtg gttaacggcg ggatataaca tgagctgtct tcggtatcgt
1561 cgtatcccac taccgagata tccgcaccaa cgcgcagccc ggactcggta atggcgcgca
1621 ttgcgcccag cgccatctga tcgttggcaa ccagcatcgc agtgggaacg atgccctcat
1681 tcagcatttg catggtttgt tgaaaaccgg acatggcact ccagtcgcct tcccgttccg
1741 ctatcggctg aatttgattg cgagtgagat atttatgcca gccagccaga cgcagacgcg
1801 ccgagacaga acttaatggg cccgctaaca gcgcgatttg ctggtgaccc aatgcgacca
1861 gatgctccac gcccagtcgc gtaccgtctt catgggagaa aataatactg ttgatgggtg
1921 tctggtcaga gacatcaaga aataacgccg gaacattagt gcaggcagct tccacagcaa
1981 tggcatcctg gtcatccagc ggatagttaa tgatcagccc actgacgcgt tgcgcgagaa
2041 gattgtgcac cgccgtttta caggcttcga cgccgcttcg ttctaccatc gacaccacca
2101 cgctggcacc cagttgatcg gcgcgagatt taatcgccgc gacaatttgc gacggcgcgt
2161 gcagggccag actggaggtg gcaacgccaa tcagcaacga ctgtttgccc gccagttgtt
2221 gtgccacgcg gttgggaatg taattcagct ccgccatcgc cgcttccact ttttcccgcg
2281 ttttcgcaga aacgtggctg gcctggttca ccacgcggga aacggtctga taagagacac
2341 cggcatactc tgcgacatcg tataacgtta ctggtttcat caaaatcgtc tccctccgtt
2401 tgaatatttg attgatcgta accagatgaa gcactctttc cactatccct acagtgttat
2461 ggcttgaaca atcacgaaac aataattggt acgtacgatc tttcagccga ctcaaacatc
2521 aaatcttaca aatgtagtct ttgaaagtat tacatatgta agatttaaat gcaaccgttt
2581 tttcggaagg aaatgatgac ctcgtttcca ccggaattag cttggtacca gctattgtaa
2641 cataatcggt acgggggtga aaaagctaac ggaaaaggga gcggaaaaga atgatgtaag
2701 cgtgaaaaat tttttatctt atcacttgaa attggaaggg agattcttta ttataagaat
2761 tgtggaattg tgagcggata acaattccca attaaaggag gaaggatcct ctagagtcga
2821 cgtccccggg gcagcccgcc taatgagcgg gcttttttca cgtcacgcgt ccatggagat
2881 ctttgtctgc aactgaaaag tttatacctt acctggaaca aatggttgaa acatacgagg
2941 ctaatatcgg cttattagga atagtccctg tactaataaa atcaggtgga tcagttgatc
3001 agtatatttt ggacgaagct cggaaagaat ttggagatga cttgcttaat tccacaatta
3061 aattaaggga aagaataaag cgatttgatg ttcaaggaat cacggaagaa gatactcatg
3121 ataaagaagc tctaaaacta ttcaataacc ttacaatgga attgatcgaa agggtggaag
3181 gttaatggta cgaaaattag gggatctacc tagaaagcca caaggcgata ggtcaagctt
3241 aaagaaccct tacatggatc ttacagattc tgaaagtaaa gaaacaacag aggttaaaca
3301 aacagaacca aaaagaaaaa aagcattgtt gaaaacaatg aaagttgatg tttcaatcca
3361 taataagatt aaatcgctgc acgaaattct ggcagcatcc gaagggaatt catattactt
3421 agaggatact attgagagag ctattgataa gatggttgag acattacctg agagccaaaa
3481 aactttttat gaatatgaat taaaaaaaag aaccaacaaa ggctgagaca gactccaaac
3541 gagtctgttt ttttaaaaaa aatattagga gcattgaata tatattagag aattaagaaa
3601 gacatgggaa taaaaatatt ttaaatccag taaaaatatg ataagattat ttcagaatat
3661 gaagaactct gtttgttttt gatgaaaaaa caaacaaaaa aaatccacct aacggaatct
3721 caatttaact aacagcggcc aaactgagaa gttaaatttg agaaggggaa aaggcggatt
3781 tatacttgta tttaactatc tccattttaa cattttatta aaccccatac aagtgaaaat
3841 cctcttttac actgttcctt taggtgatcg cggagggaca ttatgagtga agtaaaccta
3901 aaaggaaata cagatgaatt agtgtattat cgacagcaaa ccactggaaa taaaatcgcc
3961 aggaagagaa tcaaaaaagg gaaagaagaa gtttattatg ttgctgaaac ggaagagaag
4021 atatggacag aagagcaaat aaaaaacttt tctttagaca aatttggtac gcatatacct
4081 tacatagaag gtcattatac aatcttaaat aattacttct ttgatttttg gggctatttt
4141 ttaggtgctg aaggaattgc gctctatgct cacctaactc gttatgcata cggcagcaaa
4201 gacttttgct ttcctagtct acaaacaatc gctaaaaaaa tggacaagac tcctgttaca
4261 gttagaggct acttgaaact gcttgaaagg tacggtttta tttggaaggt aaacgtccgt
4321 aataaaacca aggataacac agaggaatcc ccgattttta agattagacg taaggttcct
4381 ttgctttcag aagaactttt aaatggaaac cctaatattg aaattccaga tgacgaggaa
4441 gcacatgtaa agaaggcttt aaaaaaggaa aaagagggtc ttccaaaggt tttgaaaaaa
4501 gagcacgatg aatttgttaa aaaaatgatg gatgagtcag aaacaattaa tattccagag
4561 gccttacaat atgacacaat gtatgaagat atactcagta aaggagaaat tcgaaaagaa
4621 atcaaaaaac aaatacctaa tcctacaaca tcttttgaga gtatatcaat gacaactgaa
4681 gaggaaaaag tcgacagtac tttaaaaagc gaaatgcaaa atcgtgtctc taagccttct
4741 tttgatacct ggtttaaaaa cactaagatc aaaattgaaa ataaaaattg tttattactt
4801 gtaccgagtg aatttgcatt tgaatggatt aagaaaagat atttagaaac aattaaaaca
4861 gtccttgaag aagctggata tgttttcgaa aaaatcgaac taagaaaagt gcaataaact
4921 gctgaagtat ttcagcagtt ttttttattt agaaatagtg aaaaaaatat aatcagggag
4981 gtatcaatat ttaatgagta ctgatttaaa tttatttaga ctggaattaa taattaacac
5041 gtagactaat taaaatttaa tgagggataa agaggataca aaaatattaa tttcaatccc
5101 tattaaattt taacaagggg gggattaaaa tttaattaga ggtttatcca caagaaaaga
5161 ccctaataaa atttttacta gggttataac actgattaat ttcttaatgg gggagggatt
5221 aaaatttaat gacaaagaaa acaatctttt aagaaaagct tttaaaagat aataataaaa
5281 agagctttgc gattaagcaa aactctttac tttttcattg acattatcaa attcatcgat
5341 ttcaaattgt tgttgtatca taaagttaat tctgttttgc acaacctttt caggaatata
5401 aaacacatct gaggcttgtt ttataaactc agggtcgcta aagtcaatgt aacgtagcat
5461 atgatatggt atagcttcca cccaagttag cctttctgct tcttctgaat gtttttcata
5521 tacttccatg ggtatctcta aatgattttc ctcatgtagc aaggtatgag caaaaagttt
5581 atggaattga tagttcctct ctttttcttc aactttttta tctaaaacaa acactttaac
5641 atctgagtca atgtaagcat aagatgtttt tccagtcata atttcaatcc caaatctttt
5701 agacagaaat tctggacgta aatcttttgg tgaaagaatt tttttatgta gcaatatatc
5761 cgatacagca ccttctaaaa gcgttggtga atagggcatt ttacctatct cctctcattt
5821 tgtggaataa aaatagtcat attcgtccat ctacctatcc tattatcgaa cagttgaact
5881 ttttaatcaa ggatcagtcc tttttttcat tattcttaaa ctgtgctctt aactttaaca
5941 actcgatttg tttttccaga tctcgagggt aactagcctc gccgatcccg caagaggccc
6001 ggcagtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct
6061 aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat
6121 attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg
6181 cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg
6241 aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc
6301 ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat
6361 gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact
6421 attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca
6481 tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact
6541 tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg
6601 atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg
6661 agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg
6721 aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg
6781 caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag
6841 ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc
6901 gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga
6961 tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat
7021 atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc
7081 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
7141 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
7201 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
7261 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc
7321 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
7381 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
7441 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
7501 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
7561 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
7621 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
7681 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
7741 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
7801 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta
7861 ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag
7921 tgagcgagga agcggaagag cgcccaatac gcatgc
//