LOCUS Exported File 6530 bp ds-DNA circular SYN 29-9-2015
DEFINITION .
ACCESSION .
VERSION .
KEYWORDS Untitled
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6530)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported 2015-9-29 from MLCC
http://www.miaolingbio.com
FEATURES Location/Qualifiers
source 1..6530
/organism="synthetic DNA construct"
/mol_type="other DNA"
CDS 3862..4722
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/note="AmpR"
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 4893..5481
/direction=RIGHT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
protein_bind 5769..5790
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding site"
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 5805..5835
/note="lac promoter"
/note="promoter for the E. coli lac operon"
protein_bind 5843..5859
/bound_moiety="lac repressor encoded by lacI"
/note="lac operator"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 5867..5883
/note="M13 rev"
/note="common sequencing primer, one of multiple similar
variants"
CDS 5879..6202
/codon_start=1
/gene="lacZ?fragment"
/product="LacZ-alpha fragment of beta-galactosidase"
/note="lacZ-alpha"
/translation="MTMITPSLHACRSTLEDPRVPSSNSLAVVLQRRDWENPGVTQLNR
LAAHPPFASWRNSEEARTDRPSQQLRSLNGEWRLMRYFLLTHLCGISHRIWCTLSTICS
DAA"
misc_feature 5896..5952
/gene="
"
/note="MCS"
/note="pUC18/19 multiple cloning site"
primer_bind complement(5953..5969)
/note="M13 fwd"
/note="common sequencing primer, one of multiple similar
variants"
ORIGIN
1 ccatcctcca aagttggaga gtgagtttta tgtcgcaaat attaatgttt ctggtgaacc
61 ttatcaaatt ttcgttgatt taatagaaac atagcggtaa aattagcagt aacttaatag
121 aacggaaatg aaaaaagcca ctctcatatg ctattggcta ccaaccttta gcgagaatga
181 cttaatcctg tacagccata caggacttcg acttataaga ggcgccaacc tcaaataagt
241 tatttgcctt gttttcgcga acaaggctta ttagatacac ctattgtacc gttactctac
301 gaatatttca actagtaatt actagcattg tcatatacat aataaaacgg atataaaagg
361 gcgttttcta tacctagaag tcttgtaaat gtacagggcg tttagatata gagaacgccc
421 tttttgtgtt ccgttccagt ggaggctacc actttaaaaa gatggtctag tgtagccaat
481 gcaggagagt acactcggat atcagttgtc gttgcattca actgtctgac gtaagcgagg
541 taaaggacac aagccttgca taaaacaagc ctacgggatg taaatcctaa taatgatgat
601 aaccaagacg ttagcggcaa aaagtgttgg gggttcaaaa taagacatga ttgtgcgact
661 ggagttaaac agttactcgt aagcggcgat catgacactg attcacggct attcttgtac
721 aaactttatt acaaggatat gcgggttata tagcgaatca cccgaaaggg aacggtgttg
781 ggcgtgagaa acgcaccgta cggcgcaata caatgccaat aagctatata cggacggtat
841 agtagttttg taagctataa ccgtttgtcg tcaatgcaac caatctcaat tcgagacctc
901 ggcatctaag ccagtacgaa tgagtgggcg ttttaacctc gtaaattttc aacaggggtt
961 actatgccca aaactacatt cagatttcct aacaaactcg ccagtatgaa aaccttaaga
1021 ccttaaagtc aagggatttg aaggatttta acctcgatta gcaaaaaatg tagagtactg
1081 aagcaactac cattaactaa gatagtgggg gattgaggaa gaatccagag ctgtttaaat
1141 caagtgaaag acaagatgaa attaaaagaa tagtgaaaga taggggagtg gttctctatg
1201 agaaaggaaa tggctagaga acaaaggcag cggtttattg atctattgtt agactttatg
1261 gtaaagaatc ctcatttatt tgttaatggt acagaggatg aaagtaataa tgttgttaca
1321 aaatgtaata gtgatattaa agaggttgcg gagtcatatt taactctttt atagtgagag
1381 ggttaaaact aattaatatg tattaaggcc caatgttgga attattgtat ttcactaggc
1441 aacctactta ctaaaagtaa gattatccat tagtggatgt tataatattg ggttttttaa
1501 cacaataatc atcgcctttc ggtgtcgttt gatagaaaag taaccattag cgatgaaaaa
1561 gtcaatataa aaagccatcc gtaaaaaacg gatggcttac cgtacatagg atcgttggta
1621 gggcggcgta tcctacatct ctggtaactg acctagccaa tcaaatgctt gagaacggcg
1681 gttagataag cgcgtgggga acctttccca cctcaaagat cctatatcat tattatgtta
1741 ctttctacag gtagtatacc atgttcttat attttagtaa actccccgtt agcttaacag
1801 gtctttgtaa gcaattaaac gtccactatt caatcgtctt tggattttcg caggaccgtt
1861 ttttagatcg aacatagttg ataagaacaa ataaccgctt gggtccaact ttatagcaat
1921 tagtatatgg tcatttaaaa tctttaccaa ttcaacgcta ttaggttctt taggattttg
1981 cccgacatag tcggggtgtt caacgatatc ttttatgtgc gatgaatatt tttcataaat
2041 accaggatgt tgtttcttta cgtgctttat aaatccggga aacattttta catcgttaga
2101 agtgcaagtc aagttatatg tatctataat gatttgtgga agttttgcca caacagttgg
2161 tttatttaca atcttttttt tattagccgt caaatttctc cctcatctcg tctctttata
2221 tctttatttt atcataaagg agtatttgaa ccgtcgcgcg ggacaggttt atgataggga
2281 tattttattg aataattgat ggtataaggg actttcatgc ttggaaagtg gggattatga
2341 attagatgct tgtccacaat atgttccaat gtaattaaaa tttatgttcc caccttgacc
2401 aaacatcacg tccatactta aatcgtccct cctttaatag gtaaaatatt aatttacctt
2461 aataaaaaaa taatggataa tagtattcgt ctgaatttat ataatcaggg ggaactattg
2521 atgctgggga tactatttac agcggcgcca tctactgatg tcgtaaagga tttgcaagat
2581 aaagttatat cattgcagga tcatgaggta gcgtttttga acaccacgat atctaatatg
2641 ttgatcccct tagaagcaaa cttaagagtg tgttgatagt gcagtatctt aaaattttgt
2701 gtataatagg aattgaagtt aaattagatg ctaaaaattt gtaattaaga aggagggatt
2761 cgtcatgttg gtattccaaa tgcgtaatgt agataaaaca tctactgttt tgaaacagac
2821 taaaaacagt gattacgcag ataaataaat acgttagatt aattcctacc agtgactaat
2881 cttatgactt tttaaacaga taactaaaat tacaaacaaa tcgtttaact tctgtattta
2941 tttacagatg taatcacttc aggagtaatt acatgaacaa aaatataaaa tattctcaaa
3001 actttttaac gagtgaaaaa gtactcaacc aaataataaa acaattgaat ttaaaagaaa
3061 ccgataccgt ttacgaaatt ggaacaggta aagggcattt aacgacgaaa ctggctaaaa
3121 taagtaaaca ggtaacgtct attgaattag acagtcatct attcaactta tcgacagaaa
3181 aattaaaact gaacattcgt gtcactttaa ttcaccaaga tattctacag tttcaattcc
3241 ctaacaaaca gaggtataaa attgttggga gtattcctta ccatttaagc acacaaatta
3301 ttaaaaaagt ggtttttgaa agccatgcgt ctgacatcta tctgattgtt gaagaaggat
3361 tctacaagcg taccttggat attcaccgaa cactagggtt gctcttgcac actcaagtct
3421 cgattcagca attgcttaag ctgccagcgg aatgctttca tcctaaacca aaagtaaaca
3481 gtgtcttaat aaaacttacc cgccatacca cagatgttcc agataaatat tggaagctat
3541 atacgtactt tgtttcaaaa tgggtcaatc gagaatatcg tcaactgttt actaaaaatc
3601 agtttcatca agcaatgaaa cacgccaaag taaacaattt aagtaccatt acttatgagc
3661 aagtattgtc tatttttaat agttatctat tatttaacgg gaggaaataa ttctatgagt
3721 cgctttttta aatttggaaa gttacacgtt actaaaggga atggagataa attattagat
3781 atactactga cagcttccaa gaaggtaaag aggtccctag cgcctacggg gaatttgtat
3841 cgggattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt
3901 tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat
3961 gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag
4021 atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg
4081 ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata
4141 cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat
4201 ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc
4261 aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg
4321 ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac
4381 gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact
4441 ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa
4501 gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct
4561 ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc
4621 tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga
4681 cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac
4741 tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag
4801 atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg
4861 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
4921 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
4981 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc
5041 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
5101 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
5161 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
5221 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
5281 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
5341 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
5401 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
5461 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt
5521 tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt
5581 attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag
5641 tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg
5701 ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc
5761 aacgcaatta atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt
5821 ccggctcgta tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat
5881 gaccatgatt acgccaagct tgcatgcctg caggtcgact ctagaggatc cccgggtacc
5941 gagctcgaat tcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac
6001 ccaacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc
6061 ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatggc gcctgatgcg
6121 gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatggtgca ctctcagtac
6181 aatctgctct gatgccgcat agttaagcca gccccgacac ccgccaacac ccgctgacgc
6241 gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg
6301 gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgagac gaaagggcct
6361 cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg
6421 tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc
6481 aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat
//