LOCUS Exported 8101 bp ds-DNA circular SYN 12-JUL-2016
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS pHT43-His
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 8101)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Friday, September 9, 2016 from SnapGene Viewer 3.1.4
http://www.miaolingbio.com
FEATURES Location/Qualifiers
source 1..8101
/organism="synthetic DNA construct"
/mol_type="other DNA"
primer_bind 1238..1254
/note="M13 fwd"
/note="common sequencing primer, one of multiple similar
variants"
CDS complement(1297..2379)
/codon_start=1
/gene="lacI"
/product="lac repressor"
/note="lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
/translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
YIPPSTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
ALADSLMQLARQVSRLESGQ"
protein_bind 2763..2787
/bound_moiety="lac repressor encoded by lacI"
/note="lac operator"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
misc_feature 2809..2901
/note="SP amyQ"
CDS 2959..2976
/codon_start=1
/product="6xHis affinity tag"
/note="6xHis"
/translation="HHHHHH"
promoter 6178..6282
/gene="bla"
/note="AmpR promoter"
CDS 6283..7143
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/note="AmpR"
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 7314..7902
/direction=RIGHT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 ttaagttatt ggtatgactg gttttaagcg caaaaaaagt tgctttttcg tacctattaa
61 tgtatcgttt tagaaaaccg actgtaaaaa gtacagtcgg cattatctca tattataaaa
121 gccagtcatt aggcctatct gacaattcct gaatagagtt cataaacaat cctgcatgat
181 aaccatcaca aacagaatga tgtacctgta aagatagcgg taaatatatt gaattacctt
241 tattaatgaa ttttcctgct gtaataatgg gtagaaggta attactatta ttattgatat
301 ttaagttaaa cccagtaaat gaagtccatg gaataataga aagagaaaaa gcattttcag
361 gtataggtgt tttgggaaac aatttccccg aaccattata tttctctaca tcagaaaggt
421 ataaatcata aaactctttg aagtcattct ttacaggagt ccaaatacca gagaatgttt
481 tagatacacc atcaaaaatt gtataaagtg gctctaactt atcccaataa cctaactctc
541 cgtcgctatt gtaaccagtt ctaaaagctg tatttgagtt tatcaccctt gtcactaaga
601 aaataaatgc agggtaaaat ttatatcctt cttgttttat gtttcggtat aaaacactaa
661 tatcaatttc tgtggttata ctaaaagtcg tttgttggtt caaataatga ttaaatatct
721 cttttctctt ccaattgtct aaatcaattt tattaaagtt catttgatat gcctcctaaa
781 tttttatcta aagtgaattt aggaggctta cttgtctgct ttcttcatta gaatcaatcc
841 ttttttaaaa gtcaatatta ctgtaacata aatatatatt ttaaaaatat cccactttat
901 ccaattttcg tttgttgaac taatgggtgc tttagttgaa gaataaagac cacattaaaa
961 aatgtggtct tttgtgtttt tttaaaggat ttgagcgtag cgaaaaatcc ttttctttct
1021 tatcttgata ataagggtaa ctattgccga tcgtccattc cgacagcatc gccagtcact
1081 atggcgtgct gctagcgcca ttcgccattc aggctgcgca actgttggga agggcgatcg
1141 gtgcgggcct cttcgctatt acgccagctg gcgaaagggg gatgtgctgc aaggcgatta
1201 agttgggtaa cgccagggtt ttcccagtca cgacgttgta aaacgacggc cagtgaattc
1261 gagctcaggc cttaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga
1321 aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt
1381 attgggcgcc agggtggttt ttcttttcac cagtgagacg ggcaacagct gattgccctt
1441 caccgcctgg ccctgagaga gttgcagcaa gcggtccacg ctggtttgcc ccagcaggcg
1501 aaaatcctgt ttgatggtgg ttgacggcgg gatataacat gagctgtctt cggtatcgtc
1561 gtatcccact accgagatat ccgcaccaac gcgcagcccg gactcggtaa tggcgcgcat
1621 tgcgcccagc gccatctgat cgttggcaac cagcatcgca gtgggaacga tgccctcatt
1681 cagcatttgc atggtttgtt gaaaaccgga catggcactc cagtcgcctt cccgttccgc
1741 tatcggctga atttgattgc gagtgagata tttatgccag ccagccagac gcagacgcgc
1801 cgagacagaa cttaatgggc ccgctaacag cgcgatttgc tggtgaccca atgcgaccag
1861 atgctccacg cccagtcgcg taccgtcttc atgggagaaa ataatactgt tgatgggtgt
1921 ctggtcagag acatcaagaa ataacgccgg aacattagtg caggcagctt ccacagcaat
1981 ggcatcctgg tcatccagcg gatagttaat gatcagccca ctgacgcgtt gcgcgagaag
2041 attgtgcacc gccgctttac aggcttcgac gccgcttcgt tctaccatcg acaccaccac
2101 gctggcaccc agttgatcgg cgcgagattt aatcgccgcg acaatttgcg acggcgcgtg
2161 cagggccaga ctggaggtgg caacgccaat cagcaacgac tgtttgcccg ccagttgttg
2221 tgccacgcgg ttgggaatgt aattcagctc cgccatcgcc gcttccactt tttcccgcgt
2281 tttcgcagaa acgtggctgg cctggttcac cacgcgggaa acggtctgat aagagacacc
2341 ggcatactct gcgacatcgt ataacgttac tggtttcatc aaaatcgtct ccctccgttt
2401 gaatatttga ttgatcgtaa ccagatgaag cactctttcc actatcccta cagtgttatg
2461 gcttgaacaa tcacgaaaca ataattggta cgtacgatct ttcagccgac tcaaacatca
2521 aatcttacaa atgtagtctt tgaaagtatt acatatgtaa gatttaaatg caaccgtttt
2581 ttcggaagga aatgatgacc tcgtttccac cggaattagc ttggtaccag ctattgtaac
2641 ataatcggta cgggggtgaa aaagctaacg gaaaagggag cggaaaagaa tgatgtaagc
2701 gtgaaaaatt ttttatctta tcacttgaaa ttggaaggga gattctttat tataagaatt
2761 gtggaattgt gagcggataa caattcccaa ttaaaggagg aaggatcaat gattcaaaaa
2821 cgaaagcgga cagtttcgtt cagacttgtg cttatgtgca cgctgttatt tgtcagtttg
2881 ccgattacaa aaacatcagc cgtaggatcc atgcggccgc taactagtgt caccggtatc
2941 tagagtcgac gtcccgggca tcaccatcat catcaccagc ccgcctaatg agcgggcttt
3001 tttcacgtca cgcgtccatg gagatctttg tctgcaactg aaaagtttat accttacctg
3061 gaacaaatgg ttgaaacata cgaggctaat atcggcttat taggaatagt ccctgtacta
3121 ataaaatcag gtggatcagt tgatcagtat attttggacg aagctcggaa agaatttgga
3181 gatgacttgc ttaattccac aattaaatta agggaaagaa taaagcgatt tgatgttcaa
3241 ggaatcacgg aagaagatac tcatgataaa gaagctctaa aactattcaa taaccttaca
3301 atggaattga tcgaaagggt ggaaggttaa tggtacgaaa attaggggat ctacctagaa
3361 agccacaagg cgataggtca agcttaaaga acccttacat ggatcttaca gattctgaaa
3421 gtaaagaaac aacagaggtt aaacaaacag aaccaaaaag aaaaaaagca ttgttgaaaa
3481 caatgaaagt tgatgtttca atccataata agattaaatc gctgcacgaa attctggcag
3541 catccgaagg gaattcatat tacttagagg atactattga gagagctatt gataagatgg
3601 ttgagacatt acctgagagc caaaaaactt tttatgaata tgaattaaaa aaaagaacca
3661 acaaaggctg agacagactc caaacgagtc tgttttttta aaaaaaatat taggagcatt
3721 gaatatatat tagagaatta agaaagacat gggaataaaa atattttaaa tccagtaaaa
3781 atatgataag attatttcag aatatgaaga actctgtttg tttttgatga aaaaacaaac
3841 aaaaaaaatc cacctaacgg aatctcaatt taactaacag cggccaaact gagaagttaa
3901 atttgagaag gggaaaaggc ggatttatac ttgtatttaa ctatctccat tttaacattt
3961 tattaaaccc catacaagtg aaaatcctct tttacactgt tcctttaggt gatcgcggag
4021 ggacattatg agtgaagtaa acctaaaagg aaatacagat gaattagtgt attatcgaca
4081 gcaaaccact ggaaataaaa tcgccaggaa gagaatcaaa aaagggaaag aagaagttta
4141 ttatgttgct gaaacggaag agaagatatg gacagaagag caaataaaaa acttttcttt
4201 agacaaattt ggtacgcata taccttacat agaaggtcat tatacaatct taaataatta
4261 cttctttgat ttttggggct attttttagg tgctgaagga attgcgctct atgctcacct
4321 aactcgttat gcatacggca gcaaagactt ttgctttcct agtctacaaa caatcgctaa
4381 aaaaatggac aagactcctg ttacagttag aggctacttg aaactgcttg aaaggtacgg
4441 ttttatttgg aaggtaaacg tccgtaataa aaccaaggat aacacagagg aatccccgat
4501 ttttaagatt agacgtaagg ttcctttgct ttcagaagaa cttttaaatg gaaaccctaa
4561 tattgaaatt ccagatgacg aggaagcaca tgtaaagaag gctttaaaaa aggaaaaaga
4621 gggtcttcca aaggttttga aaaaagagca cgatgaattt gttaaaaaaa tgatggatga
4681 gtcagaaaca attaatattc cagaggcctt acaatatgac acaatgtatg aagatatact
4741 cagtaaagga gaaattcgaa aagaaatcaa aaaacaaata cctaatccta caacatcttt
4801 tgagagtata tcaatgacaa ctgaagagga aaaagtcgac agtactttaa aaagcgaaat
4861 gcaaaatcgt gtctctaagc cttcttttga tacctggttt aaaaacacta agatcaaaat
4921 tgaaaataaa aattgtttat tacttgtacc gagtgaattt gcatttgaat ggattaagaa
4981 aagatattta gaaacaatta aaacagtcct tgaagaagct ggatatgttt tcgaaaaaat
5041 cgaactaaga aaagtgcaat aaactgctga agtatttcag cagttttttt tatttagaaa
5101 tagtgaaaaa aatataatca gggaggtatc aatatttaat gagtactgat ttaaatttat
5161 ttagactgga attaataatt aacacgtaga ctaattaaaa tttaatgagg gataaagagg
5221 atacaaaaat attaatttca atccctatta aattttaaca agggggggat taaaatttaa
5281 ttagaggttt atccacaaga aaagacccta ataaaatttt tactagggtt ataacactga
5341 ttaatttctt aatgggggag ggattaaaat ttaatgacaa agaaaacaat cttttaagaa
5401 aagcttttaa aagataataa taaaaagagc tttgcgatta agcaaaactc tttacttttt
5461 cattgacatt atcaaattca tcgatttcaa attgttgttg tatcataaag ttaattctgt
5521 tttgcacaac cttttcagga atataaaaca catctgaggc ttgttttata aactcagggt
5581 cgctaaagtc aatgtaacgt agcatatgat atggtatagc ttccacccaa gttagccttt
5641 ctgcttcttc tgaatgtttt tcatatactt ccatgggtat ctctaaatga ttttcctcat
5701 gtagcaaggt atgagcaaaa agtttatgga attgatagtt cctctctttt tcttcaactt
5761 ttttatctaa aacaaacact ttaacatctg agtcaatgta agcataagat gtttttccag
5821 tcataatttc aatcccaaat cttttagaca gaaattctgg acgtaaatct tttggtgaaa
5881 gaattttttt atgtagcaat atatccgata cagcaccttc taaaagcgtt ggtgaatagg
5941 gcattttacc tatctcctct cattttgtgg aataaaaata gtcatattcg tccatctacc
6001 tatcctatta tcgaacagtt gaacttttta atcaaggatc agtccttttt ttcattattc
6061 ttaaactgtg ctcttaactt taacaactcg atttgttttt ccagatctcg agggtaacta
6121 gcctcgccga tcccgcaaga ggcccggcag tcaggtggca cttttcgggg aaatgtgcgc
6181 ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa
6241 taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc
6301 cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa
6361 acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa
6421 ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg
6481 atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa
6541 gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc
6601 acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc
6661 atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta
6721 accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag
6781 ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca
6841 acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata
6901 gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc
6961 tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca
7021 ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca
7081 actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg
7141 taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa
7201 tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt
7261 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
7321 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
7381 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
7441 gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac
7501 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
7561 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
7621 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
7681 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
7741 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
7801 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
7861 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
7921 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc
7981 cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc
8041 cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcatg
8101 c
//