LOCUS Exported 8717 bp ds-DNA circular SYN 10-AUG-2017
DEFINITION synthetic circular DNA
FEATURES Location/Qualifiers
source 1..8717
/organism="synthetic DNA construct"
/mol_type="other DNA"
CDS complement(823..1905)
/codon_start=1
/gene="lacI"
/product="lac repressor"
/label=lacI
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
/translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
YIPPSTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
ALADSLMQLARQVSRLESGQ"
protein_bind 2514..2530
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
rep_origin complement(6888..7476)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(7647..8507)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(8508..8612)
/gene="bla"
/label=AmpR promoter
ORIGIN
1 aacaaaattc tccagtcttc acatcggttt gaaaggagga agcggaagaa tgaagtaaga
61 gggatttttg actccgaagt aagtcttcaa aaaatcaaat aaggagtgtc aagaatgttt
121 gcaaaacgat tcaaaacctc tttactgccg ttattcgctg gatttttatt gctgtttcat
181 ttggttctgg caggaccggc ggctgcgagt gctgaaacgg cgaacaaatc gaatgagctt
241 acagcaccgt cgatcaaaag cggaaccatt cttcatgcat ggaattggtc gttcaatacg
301 ttaaaacaca atatgaagga tattcatgat gcaggatata cagccattca gacatctccg
361 attaaccaag taaaggaagg gaatcaagga gataaaagca tgtcgaactg gtactggctg
421 tatcagccga catcgtatca aattggcaac cgttacttag gtactgaaca agaatttaaa
481 gaaatgtgtg cagccgctga agaatatggc ataaaggtca ttgttgacgc ggtcatcaat
541 cataccacca gtgattatgc cgcgatttcc aatgaggtta agagtattcc aaactggaca
601 catggaaaca cacaaattaa aaactggtct gatcggatcc gcggctagcc atggtcgacg
661 cggccgcaaa attgaaaaaa tggtggaaac acttttttca atttttttgt tttattattt
721 aatatttggg aaatattcat tctaattggt aatcagattt tagaaaacaa taaacccttg
781 cataggggga tcggatccta actcacatta attgcgttgc gctcactgcc cgctttccag
841 tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt
901 ttgcgtattg ggcgccaggg tggtttttct tttcaccagt gagacgggca acagctgatt
961 gcccttcacc gcctggccct gagagagttg cagcaagcgg tccacgctgg tttgccccag
1021 caggcgaaaa tcctgtttga tggtggttga cggcgggata taacatgagc tgtcttcggt
1081 atcgtcgtat cccactaccg agatatccgc accaacgcgc agcccggact cggtaatggc
1141 gcgcattgcg cccagcgcca tctgatcgtt ggcaaccagc atcgcagtgg gaacgatgcc
1201 ctcattcagc atttgcatgg tttgttgaaa accggacatg gcactccagt cgccttcccg
1261 ttccgctatc ggctgaattt gattgcgagt gagatattta tgccagccag ccagacgcag
1321 acgcgccgag acagaactta atgggcccgc taacagcgcg atttgctggt gacccaatgc
1381 gaccagatgc tccacgccca gtcgcgtacc gtcttcatgg gagaaaataa tactgttgat
1441 gggtgtctgg tcagagacat caagaaataa cgccggaaca ttagtgcagg cagcttccac
1501 agcaatggca tcctggtcat ccagcggata gttaatgatc agcccactga cgcgttgcgc
1561 gagaagattg tgcaccgccg ctttacaggc ttcgacgccg cttcgttcta ccatcgacac
1621 caccacgctg gcacccagtt gatcggcgcg agatttaatc gccgcgacaa tttgcgacgg
1681 cgcgtgcagg gccagactgg aggtggcaac gccaatcagc aacgactgtt tgcccgccag
1741 ttgttgtgcc acgcggttgg gaatgtaatt cagctccgcc atcgccgctt ccactttttc
1801 ccgcgttttc gcagaaacgt ggctggcctg gttcaccacg cgggaaacgg tctgataaga
1861 gacaccggca tactctgcga catcgtataa cgttactggt ttcatcaaaa tcgtctccct
1921 ccgtttgaat atttgattga tcgtaaccag atgaagcact ctttccacta tccctacagt
1981 gttatggctt gaacaatcac gaaacaataa ttggtacgta cgatctttca gccgactcaa
2041 acatcaaatc ttacaaatgt agtctttgaa agtattacat atgtaagatt taaatgcaac
2101 cgttttttcg gaaggaaatg atgacctcgt ttccaccgga attagcttgc atgcctaccc
2161 aatcagtacg ttaattttgg ctttaatgag ttgtaattcc tctggggcaa ctgttccttt
2221 cttcgttgct cctcttgccc gccaggcgat actttttacc tgatcagcta acgctacgcc
2281 atcacgttcc tgaccggata aaacaacttc gaacggatat ccttttgatt gcgttgtaca
2341 aggaacacac agacacatac ctgttttgtt gttgtacatg aaaggactca ggacaacagc
2401 tggacgatgt ccagcttgct cgctaccttt tgtcgggtca aaatcaaccc aaatcagatc
2461 gcccatatcg ggtacgtatc ggcttaccat acctgcttcc tccttaagct taattgttat
2521 ccgctcacaa ttccacacat tatgccacac cttgtagata aagtcaacaa ctttttgcaa
2581 aatttttcag gaattttagc agaggttgtt ctggatgtag aacaaaacat ctttccgctc
2641 ttgtgctgtt aggatatctt tcttggaagc taggtaggca agggctacct caaataaatc
2701 ttcttcaggg tgcgctattt ttaaggtgcc tagtgaggtc ttgaccactt cacccataat
2761 ttcagtgccg aatagtctgg actgggctgt gtagaattcc tgcagccctg gcgaatggcg
2821 attttcgttc gtgaatacat gttataataa ctataactaa taacgtaacg tgactggcaa
2881 gagatatttt taaaacaatg aataggttta cacttacttt agttttatgg aaatgaaaga
2941 tcatatcata tataatctag aataaaatta actaaaataa ttattatcta gataaaaaat
3001 ttagaagcca atgaaatcta taaataaact aaattaagtt tatttaatta acaactatgg
3061 atataaaata ggtactaatc aaaatagtga ggaggatata tttgaataca tacgaacaaa
3121 ttaataaagt gaaaaaaata cttcggaaac atttaaaaaa taaccttatt ggtacttaca
3181 tgtttggatc aggagttgag agtggactaa aaccaaatag tgatcttgac tttttagtcg
3241 tcgtatctga accattgaca gatcaaagta aagaaatact tatacaaaaa attagaccta
3301 tttcaaaaaa aataggagat aaaagcaact tacgatatat tgaattaaca attattattc
3361 agcaagaaat ggtaccgtgg aatcatcctc ccaaacaaga atttatttat ggagaatggt
3421 tacaagagct ttatgaacaa ggatacattc ctcagaagga attaaattca gatttaacca
3481 taatgcttta ccaagcaaaa cgaaaaaata aaagaatata cggaaattat gacttagagg
3541 aattactacc tgatattcca ttttctgatg tgagaagagc cattatggat tcgtcagagg
3601 aattaataga taattatcag gatgatgaaa ccaactctat attaacttta tgccgtatga
3661 ttttaactat ggacacgggt aaaatcatac caaaagatat tgcgggaaat gcagtggctg
3721 aatcttctcc attagaacat agggagagaa ttttgttagc agttcgtagt tatcttggag
3781 agaatattga atggactaat gaaaatgtaa atttaactat aaactattta aataacagat
3841 taaaaaaatt ataaaaaaat tgaaaaaatg gtggaaacac ttttttcaat ttttttgttt
3901 tattatttaa tatttgggaa atattcattc taattggtaa tcagatttta gaaaacaata
3961 aacccttgca tagggggatc tcgacatgga tgagcgatga tgatatccgt ttaggctggg
4021 cggtgatagc ttctcgttca ggcagtacgc ctcttttctt ttccagacct gagggaggcg
4081 gaaatggtgt gaggttcccg gggaaaagcc aaataggcga tcgcgggagt gctttatttg
4141 aagatcaggc tatcactgcg gtcaatagat ttcacaatgt gatggctgga cagcctgagg
4201 aactctcgaa cccgaatgga aacaaccaga tatttatgaa tcagcgcggc tcacatggcg
4261 ttgtgctggc aaatgcaggt tcatcctctg tctctatcaa tacggcaaca aaattgcctg
4321 atggcaggta tgacaataaa gctggagcgg gttcatttca agtgaacgat ggtaaactga
4381 caggcacgat caatgccagg tctgtagctg tgctttatcc tgatgatatt gcaaaagcgc
4441 ctcatgtttt ccttgagaat tacaaaacag gtgtaacaca ttctttcaat gatcaactga
4501 cgattacctt gcgtgcagat gcgaatacaa caaaagccgt ttatcaaatc aataatggac
4561 cagacgacag gcgtttaagg atggagatca attcacaatc ggaaaaggag atccaatttg
4621 gcaaaacata caccatcatg ttaaaaggaa cgaacagtga tggtgtaacg aggaccgaga
4681 aatacagttt tgttaaaaga gatccagcgt cggccaaaac catcggctat caaaatccga
4741 atcattggag ccaggtaaat gcttatatct ataaacatga tgggagccga gtaattgaat
4801 tgaccggatc ttggcctgga aaaccaatga ctaaaaatgc agacggaatt tacacgctga
4861 cgctgcctgc ggacacggat acaaccaacg caaaagtgat ttttaataat ggcagcgccc
4921 aagtgcccgg tcagaatcag cctggctttg attacgtgct aaatggttta tataatgact
4981 cgggcttaag cggttctctt ccccattgag ggcaaggcta gacgggactt accgaaagaa
5041 accatcaatg atggtttctt ttttgttcat aaatcagaca aaacttttct cttgcaaaag
5101 tttgtgaagt gttgcacaat ataaatgtga aatacttcac aaacaaaaag acatcaaaga
5161 gaaacatacc ctgcaaggat gctgatattg tctgcatttg cgccggagca aaccaaaaac
5221 ctggtgagac acgccttgaa ttagtagaaa agaacttgaa gattttcaaa ggcatcgtta
5281 gtgaagtcat ggcgagcgga tttgacggca ttttcttagt cgcgacgcga ggctggatgg
5341 ccttccccat tatgattctt ctcgcttccg gcggcatcgg gatgcccgcg ttgcaggcca
5401 tgctgtccag gcaggtagat gacgaccatc agggacagct tcaaggatcg ctcgcggctc
5461 ttaccagcct aacttcgatc actggaccgc tgatcgtcac ggcgatttat gccgcctcgg
5521 cgagcacatg gaacgggttg gcatggattg taggcgccgc cctatacctt gtctgcctcc
5581 ccgcgttgcg tcgcggtgca tggagccggg ccacctactg aagtggattt ctttaagagc
5641 tccgataacc agaagcaata aaaaatcaaa tcggatttca ctatataatc tcactttatc
5701 taagatgaat ccgatggaag catcctgttt tctctcaatt tttttatcta aaacccagcg
5761 ttcgatgctt ctttgagcga acgatcaaaa ataagtgcct tcccatcaaa aaaatattct
5821 caacataaaa aactttgtgt aatacttgta acgctacatg gagattaact caatctagag
5881 ggtattaata atgatccaca gtagcgtaaa gcgttgggga aattcaccgg cggtgcggat
5941 cccggctacg ttaatgcagg cgctcaatct gaatattgat gatgaagtga agattgacct
6001 ggtggatggc aaattaatta ttgagccagt gcgtaaagag cccgtattta cgcttgctga
6061 actggtcaac gacatcacgc cggaaaacct ccacgagaat atcgactggg gagagccgaa
6121 agataaggaa gtctggtaaa tcgattatgt cttttgcgca gtcggcttaa accagttttc
6181 gctggtgcga aaaaagagtg tcttgtgaca cctaaattca aaatctatcg gtcagattta
6241 taccgatttg attttatata ttcttgaata acatacgccg agttatcaca taaaagcggg
6301 aaccaatcat caaatttaaa cttcattgca taatccatta aactcttaaa ttctacgatt
6361 ccttgttcat caataaactc aatcatttct ttaattaatt tatatctatc tgttgttgtt
6421 ttctttaata attcatcaac atctacaccg ccataaacta tcatatcttc tttttgatat
6481 ttaaatttat taggatctta aggcctaggt ctagagtctt tgttttgacg ccattagcgt
6541 acgtaacaat cctcgttaaa ggacaaggac ctgagcggaa gtgtatcgta cagtagacgg
6601 agtatactag tatagtctat agtccgtgga attattatat ttatctccga cgatattctc
6661 atcagtgaaa tccagctgga gttctttagc aaattttttt attagctgaa cttagtatta
6721 gtggccatac tcctccaatc caaagctatt tagaaagatt actatatcct caaacaggcg
6781 gtaaccggcc tcttcatcgg gaatgcgcgc gaccttcagc atcgccggca tgtccccctg
6841 gcggacggga agtatccagc tcgaggtcgg gccgcgttgc tggcgttttt ccataggctc
6901 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
6961 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
7021 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
7081 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
7141 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
7201 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
7261 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
7321 actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
7381 gttgatagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
7441 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
7501 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca
7561 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt
7621 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca
7681 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
7741 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca
7801 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
7861 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
7921 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctgcaggcat cgtggtgtca
7981 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
8041 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga
8101 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
8161 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
8221 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caacacggga taataccgcg
8281 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
8341 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
8401 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
8461 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
8521 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
8581 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac
8641 gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc
8701 tttcgtcttc aagaatt
//