LOCUS Exported 6072 bp ds-DNA circular SYN 23-MAY-2016
DEFINITION synthetic circular DNA
KEYWORDS pAC5.1b-EGFP
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6072)
TITLE Direct Submission
JOURNAL Exported Monday, May 23, 2016 from MiaoLingPlasmid
http://www.miaolingbio.com
FEATURES Location/Qualifiers
source 1..6072
/organism="synthetic DNA construct"
/mol_type="other DNA"
promoter 56..2568
/note="Ac5 promoter"
/note="Drosophila melanogaster actin 5C promoter"
CDS 2617..3339
/codon_start=1
/product="enhanced GFP"
/note="EGFP"
/note="mammalian codon-optimized"
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
EFVTAAGITLGMDELYKF"
CDS 3357..3398
/codon_start=1
/product="epitope tag from simian virus 5"
/note="V5 tag"
/translation="GKPIPNPLLGLDST"
CDS 3408..3425
/codon_start=1
/product="6xHis affinity tag"
/note="6xHis"
/translation="HHHHHH"
polyA_signal 3498..3632
/note="SV40 poly(A) signal"
/note="SV40 polyadenylation signal"
rep_origin complement(4254..4842)
/direction=LEFT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(5013..5873)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/note="AmpR"
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(5874..5978)
/gene="bla"
/note="AmpR promoter"
ORIGIN
1 cttcaaggaa ttaattctat attctaaaaa cacaaatgat acttctaaaa aaaatcatga
61 atggcatcaa ctctgaatca aatctttgca gatgcaccta cttctcattt ccactgtcac
121 atcatttttc cagatctcgc tgcctgttat gtggcccaca aaccaagaca cgttttatgg
181 ccattaaagc tggctgatcg tcgccaaaca ccaaatacat atcaatatgt acattcgaga
241 aagaagcgat caaagaagcg tcttcgggcg agtaggagaa tgcggaggag aaggagaacg
301 agctgatcta gtatctctcc acaatccaat gccaactgac caactggcca tattcggagc
361 aatttgaagc caatttccat cgcctggcga tcgctccatt cttggctata tgtttttcac
421 cgttcccggg gccattttca aagactcgtc ggtaagataa gattgtgtca ctcgctgtct
481 ctcttcattt gtcgaagaat gctgaggaat ttcgcgatga cgtcggcgag tattttgaag
541 aatgagaata atttgtattt atacgaaaat cagttagtgg aattttctac aaaaacatgt
601 tatctataga taattttgtt gcaaaatatg ttgactatga caaagattgt atgtatatac
661 ctttaatgta ttctcatttt cttatgtatt tataatggca atgatgatac tgatgatatt
721 ttaagatgat gccagaccac aggctgattt ctgcgtcttt tgccgaacgc agtgcatgtg
781 cggttgttgt tttttggaat agtttcaatt ttcggactgt ccgctttgat ttcagtttct
841 tggcttattc aaaaagcaaa gtaaagccaa aaaagcgaga tggcaatacc aaatgcggca
901 aaacggtagt ggaaggaaag gggtgcgggg cagcggaagg aagggtgggg cggggcgtgg
961 cggggtctgt ggctgggcgc gacgtcaccg acgttggagc cactcctttg accatgtgtg
1021 cgtgtgtgta ttattcgtgt ctcgccactc gccggttgtt tttttctttt tatctcgctc
1081 tctctagcgc catctcgtac gcatgctcaa cgcaccgcat gttgccgtgt cctttatgcg
1141 tcattttggc tcgaaatagg caattattta aacaaagatt agtcaacgaa aacgctaaaa
1201 taaataagtc tacaatatgg ttacttattg ccatgtgtgt gcagccaacg atagcaacaa
1261 aagcaacaac acagtggctt tccctctttc actttttgtt tgcaagcgcg tgcgagcaag
1321 acggcacgac cggcaaacgc aattacgctg acaaagagca gacgaagttt tggccgaaaa
1381 acatcaaggc gcctgatacg aatgcatttg caataacaat tgcgatattt aatattgttt
1441 atgaagctgt ttgacttcaa aacacacaaa aaaaaaaata aaacaaatta tttgaaagag
1501 aattaggaat cggacagctt atcgttacgg gctaacagca caccgagacg aaatagctta
1561 cctgacgtca cagcctctgg aagaactgcc gccaagcaga gagagagaga aaaagaggga
1621 gagcagctta gaccgcatgt gcttgtgtgt gaggcgtctc tctcttcgtc tcctgtttgc
1681 gcaaacgcat agactgcact gagaaaatcg attacctatt ttttatgaat gaatatttgc
1741 actattacta ttcaaaacta ttaagatagc aatcacattc aatagccaaa tactatacca
1801 cctgagcgat gcaacgaaat gatcaatttg agcaaaaatg ctgcatattt aggacggcat
1861 cattatagaa atgcttcttg ctgtgtactt ttctctcgtc tggcagctgt ttcgccgtta
1921 ttgttaaaac cggcttaagt taggtgtgtt ttctacgact agtgatgccc ctactagaag
1981 atgtgtgttg cacaaatgtc cctgaataac caatttgaag tgcagatagc agtaaacgta
2041 agctaatatg aatattattt aactgtaatg ttttaatatc gctggacatt actaataaac
2101 ccactataaa cacatgtaca tatgtatgtt ttggcataca atgagtagtt ggggaaaaaa
2161 tgtgtaaaag caccgtgacc atcacagcat aaagataacc agctgaagta tcgaatatga
2221 gtaaccccca aattgaatca catgccgcaa ctgataggac ccatggaagt acactcttca
2281 tggcgatata caagacacac acaagcacga acacccagtt gcggaggaaa ttctccgtaa
2341 atgaaaaccc aatcggcgaa caattcatac ccatatatgg taaaagtttt gaacgcgact
2401 tgagagcgga gagcattgcg gctgataagg ttttagcgct aagcgggctt tataaaacgg
2461 gctgcgggac cagttttcat atcactaccg tttgagttct tgtgctgtgt ggatactcct
2521 cccgacacaa agccgctcca tcagccagca gtcgtctaat ccagagaccc cggatcgggg
2581 tacctactag tccagtgtgg tggaatttcg agctttatgg tgagcaaggg cgaggagctg
2641 ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc
2701 agcgtgtccg gcgagggcga gggcgatgcc acctacggca agctgaccct gaagttcatc
2761 tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct gacctacggc
2821 gtgcagtgct tcagccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc
2881 atgcccgaag gctacgtcca ggagcgcacc atcttcttca aggacgacgg caactacaag
2941 acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc
3001 atcgacttca aggaggacgg caacatcctg gggcacaagc tggagtacaa ctacaacagc
3061 cacaacgtct atatcatggc cgacaagcag aagaacggca tcaaggtgaa cttcaagatc
3121 cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc actaccagca gaacaccccc
3181 atcggcgacg gccccgtgct gctgcccgac aaccactacc tgagcaccca gtccgccctg
3241 agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc
3301 gggatcactc tcggcatgga cgagctgtac aagttctaga gggcccgcgg ttcgaaggta
3361 agcctatccc taaccctctc ctcggtctcg attctacgcg taccggtcat catcaccatc
3421 accattgagt ttaaacccgc tgatcagcct cgactgtgcc ttctaaggcc tgagctcgct
3481 gatcagcctc gatcgaggat ccagacatga taagatacat tgatgagttt ggacaaacca
3541 caactagaat gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct attgctttat
3601 ttgtaaccat tataagctgc aataaacaag ttaacaacaa caattgcatt cattttatgt
3661 ttcaggttca gggggaggtg tgggaggttt tttaaagcaa gtaaaacctc tacaaatgtg
3721 gtatggctga ttatgatcag tcgaccgatg cccttgagag ccttcaaccc agtcagctcc
3781 ttccggtggg cgcggggcat gactatcgtc gccgcactta tgactgtctt ctttatcatg
3841 caactcgtag gacaggtgcc ggcagcgctc tgggtcattt tcggcgagga ccgctttcgc
3901 tggagcgcga cgatgatcgg cctgtcgctt gcggtattcg gaatcttgca cgccctcgct
3961 caagccttcg tcactggtcc cgccaccaaa cgtttcggcg agaagcaggc cattatcgcc
4021 ggcatggcgg ccgacgcgct gggctacgtc ttgctggcgt tcgcgacgcg aggctggatg
4081 gccttcccca ttatgattct tctcgcttcc ggcggcatcg ggatgcccgc gttgcaggcc
4141 atgctgtcca ggcaggtaga tgacgaccat cagggacagc ttcaaggatc gctcgcggct
4201 cttaccagcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
4261 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
4321 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
4381 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
4441 ctttctcaat gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
4501 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
4561 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
4621 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
4681 ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
4741 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
4801 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
4861 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
4921 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
4981 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
5041 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
5101 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca
5161 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
5221 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
5281 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctgc aggcatcgtg
5341 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
5401 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
5461 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
5521 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
5581 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaac acgggataat
5641 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
5701 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
5761 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
5821 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
5881 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
5941 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
6001 cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg
6061 aggccctttc gt
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