LOCUS Exported 4857 bp ds-DNA circular SYN 09-SEP-2016
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS Untitled
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 4857)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Friday, September 9, 2016 from SnapGene Viewer 3.1.4
http://www.miaolingbio.com
FEATURES Location/Qualifiers
source 1..4857
/organism="synthetic DNA construct"
/mol_type="other DNA"
rep_origin 2..457
/direction=RIGHT
/note="f1 ori"
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 484..588
/gene="bla"
/note="AmpR promoter"
CDS 589..1449
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/note="AmpR"
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 1620..2208
/direction=RIGHT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
mobile_element 2511..2735
/mobile_element_type="transposon:Tn7"
/note="Tn7R"
/note="mini-Tn7 element (right end of the Tn7 transposon)"
CDS complement(2802..3335)
/codon_start=1
/gene="aacC1"
/product="gentamycin acetyltransferase"
/note="GmR"
/note="confers resistance to gentamycin"
/translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPRFEQPRS
EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
EEVMHFDIDPSTAT"
promoter complement(3524..3552)
/gene="intI1 (promoter lies within the coding sequence)"
/note="Pc promoter"
/note="class 1 integron promoter"
promoter 3904..3995
/gene="polh from Autographa californica"
/note="polyhedrin promoter"
/note="promoter for the baculovirus polyhedrin gene"
CDS 4062..4079
/codon_start=1
/product="6xHis affinity tag"
/note="6xHis"
/translation="HHHHHH"
CDS 4101..4121
/codon_start=1
/product="tobacco etch virus (TEV) protease recognition and
cleavage site"
/note="TEV site"
/translation="ENLYFQG"
polyA_signal 4347..4481
/note="SV40 poly(A) signal"
/note="SV40 polyadenylation signal"
mobile_element 4510..4675
/mobile_element_type="transposon:Tn7"
/note="Tn7L"
/note="mini-Tn7 element (left end of the Tn7 transposon)"
ORIGIN
1 gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc
61 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc
121 acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt
181 agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg
241 ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt
301 ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta
361 taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt
421 aacgcgaatt ttaacaaaat attaacgttt acaatttcag gtggcacttt tcggggaaat
481 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg
541 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa
601 catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac
661 ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac
721 atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt
781 ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc
841 gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca
901 ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc
961 ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag
1021 gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa
1081 ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg
1141 gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa
1201 ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg
1261 gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt
1321 gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt
1381 caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag
1441 cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat
1501 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct
1561 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
1621 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
1681 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
1741 agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc
1801 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
1861 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
1921 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
1981 tacaccgaac tgagatacct acagcgtgag cattgagaaa gcgccacgct tcccgaaggg
2041 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
2101 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
2161 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
2221 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
2281 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
2341 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg
2401 cggtattttc tccttacgca tctgtgcggt atttcacacc gcagaccagc cgcgtaacct
2461 ggcaaaatcg gttacggttg agtaataaat ggatgccctg cgtaagcggg tgtgggcgga
2521 caataaagtc ttaaactgaa caaaatagat ctaaactatg acaataaagt cttaaactag
2581 acagaatagt tgtaaactga aatcagtcca gttatgctgt gaaaaagcat actggacttt
2641 tgttatggct aaagcaaact cttcattttc tgaagtgcaa attgcccgtc gtattaaaga
2701 ggggcgtggc caagggcatg gtaaagacta tattcgcggc gttgtgacaa tttaccgaac
2761 aactccgcgg ccgggaagcc gatctcggct tgaacgaatt gttaggtggc ggtacttggg
2821 tcgatatcaa agtgcatcac ttcttcccgt atgcccaact ttgtatagag agccactgcg
2881 ggatcgtcac cgtaatctgc ttgcacgtag atcacataag caccaagcgc gttggcctca
2941 tgcttgagga gattgatgag cgcggtggca atgccctgcc tccggtgctc gccggagact
3001 gcgagatcat agatatagat ctcactacgc ggctgctcaa acctgggcag aacgtaagcc
3061 gcgagagcgc caacaaccgc ttcttggtcg aaggcagcaa gcgcgatgaa tgtcttacta
3121 cggagcaagt tcccgaggta atcggagtcc ggctgatgtt gggagtaggt ggctacgtct
3181 ccgaactcac gaccgaaaag atcaagagca gcccgcatgg atttgacttg gtcagggccg
3241 agcctacatg tgcgaatgat gcccatactt gagccaccta actttgtttt agggcgactg
3301 ccctgctgcg taacatcgtt gctgctgcgt aacatcgttg ctgctccata acatcaaaca
3361 tcgacccacg gcgtaacgcg cttgctgctt ggatgcccga ggcatagact gtacaaaaaa
3421 acagtcataa caagccatga aaaccgccac tgcgccgtta ccaccgctgc gttcggtcaa
3481 ggttctggac cagttgcgtg agcgcatacg ctacttgcat tacagtttac gaaccgaaca
3541 ggcttatgtc aactgggttc gtgccttcat ccgtttccac ggtgtgcgtc acccggcaac
3601 cttgggcagc agcgaagtcg aggcatttct gtcctggctg gcgaacgagc gcaaggtttc
3661 ggtctccacg catcgtcagg cattggcggc cttgctgttc ttctacggca aggtgctgtg
3721 cacggatctg ccctggcttc aggagatcgg aagacctcgg ccgtcgcggc gcttgccggt
3781 ggtgctgacc ccggatgaag tggttcgcat cctcggtttt ctggaaggcg agcatcgttt
3841 gttcgcccag gactctagct atagttctag tggttggcta cgtatactcc ggaatattaa
3901 tagatcatgg agataattaa aatgataacc atctcgcaaa taaataagta ttttactgtt
3961 ttcgtaacag ttttgtaata aaaaaaccta taaatattcc ggattattca taccgtccca
4021 ccatcgggcg cggatctcgg tccgaaacca tgtcgtacta ccatcaccat caccatcacg
4081 attacgatat cccaacgacc gaaaacctgt attttcaggg cgccatggga tccggaattc
4141 aaaggcctac gtcgacgagc tcactagtcg cggccgcttt cgaatctaga gcctgcagtc
4201 tcgaggcatg cggtaccaag cttgtcgaga agtactagag gatcataatc agccatacca
4261 catttgtaga ggttttactt gctttaaaaa acctcccaca cctccccctg aacctgaaac
4321 ataaaatgaa tgcaattgtt gttgttaact tgtttattgc agcttataat ggttacaaat
4381 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg
4441 gtttgtccaa actcatcaat gtatcttatc atgtctggat ctgatcactg atatcgccta
4501 ggagatccga accagataag tgaaatctag ttccaaacta ttttgtcatt tttaattttc
4561 gtattagctt acgacgctac acccagttcc catctatttt gtcactcttc cctaaataat
4621 ccttaaaaac tccatttcca cccctcccag ttcccaacta ttttgtccgc ccacagcggg
4681 gcatttttct tcctgttatg tttttaatca aacatcctgc caactccatg tgacaaaccg
4741 tcatcttcgg ctactttttc tctgtcacag aatgaaaatt tttctgtcat ctcttcgtta
4801 ttaatgtttg taattgactg aatatcaacg cttatttgca gcctgaatgg cgaatgg
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