LOCUS Exported 8328 bp ds-DNA circular SYN 15-JUN-2017
DEFINITION synthetic circular DNA
KEYWORDS pVE5523
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 8328)
TITLE Direct Submission
JOURNAL Exported Thursday, June 15, 2017 from SnapGene Viewer 3.3.4
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..8328
/organism="synthetic DNA construct"
/mol_type="other DNA"
primer_bind complement(2819..2835)
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
promoter 3309..3413
/gene="bla"
/label=AmpR promoter
CDS 3414..4274
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 4445..5033
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
protein_bind 5321..5342
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 5357..5387
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 5395..5411
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 5419..5435
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
promoter 5456..5474
/label=T3 promoter
/note="promoter for bacteriophage T3 RNA polymerase"
terminator 6358..6444
/gene="Escherichia coli rrnB"
/label=rrnB T1 terminator
/note="transcription terminator T1 from the E. coli rrnB
gene"
terminator 6463..6490
/label=rrnB T2 terminator
/note="transcription terminator T2 from the E. coli rrnB
gene"
CDS 7104..7841
/codon_start=1
/label=Ery
/translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLTT
KLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGSIPYH
LSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAECFH
PKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHAKVNN
LSTVTYEQVLSIFNSYLLFNGRK"
ORIGIN
1 aactcaacgc tagtagtgga tttaatccca aatgagccaa cagaaccaga accagaaaca
61 gaatcagaac aagtaacatt ggatttagaa atggaagaag aaaaaagcaa tgacttcgtg
121 tgaataatgc acgaaatcgt tgcttatttt tttttaaaag cggtatacta gatataacga
181 aacaacgaac tgaatagaaa cgaaaaaaga gccatgacac atttataaaa tgtttgacga
241 cattttataa atgcatagcc cgataagatt gccaaaccaa cgcttatcag ttagtcagat
301 gaactcttcc ctcgtaagaa gttatttaat taactttgtt tgaagacggt atataaccgt
361 actatcatta tatagggaaa tcagagagtt ttcaagtatc taagctactg aatttaagaa
421 ttgttaagca atcaatcgga aatcgtttga ttgctttttt tgtattcatt tatagaaggt
481 ggagtttgta tgaatcatga tgaatgtaaa acttatataa aaaatagttt attggagata
541 agaaaattag caaatatcta tacactagaa acgtttaaga aagagttaga aaagagaaat
601 atctacttag aaacaaaatc agataagtat ttttcttcgg agggggaaga ttatatatat
661 aagttaatag aaaataacaa aataatttat tcgattagtg gaaaaaaatt gacttataaa
721 ggaaaaaaat ctttttcaaa acatgcaata ttgaaacagt tgaatgaaaa agcaaaccaa
781 gttaattaaa caacctattt tataggattt ataggaaagg agaacagctg aatgaatatc
841 ccttttgttg tagaaactgt gcttcatgac ggcttgttaa agtacaaatt taaaaatagt
901 aaaattcgct caatcactac caagccaggt aaaagcaaag gggctatttt tgcgtatcgc
961 tcaaaatcaa gcatgattgg cggtcgtggt gttgttctga cttccgagga agcgattcaa
1021 gaaaatcaag atacatttac acattggaca cccaacgttt atcgttatgg aacgtatgca
1081 gacgaaaacc gttcatacac gaaaggacat tctgaaaaca atttaagaca aatcaatacc
1141 ttctttattg attttgatat tcacacggca aaagaaacta tttcagcaag cgatatttta
1201 acaaccgcta ttgatttagg ttttatgcct actatgatta tcaaatctga taaaggttat
1261 caagcatatt ttgttttaga aacgccagtc tatgtgactt caaaatcaga atttaaatct
1321 gtcaaagcag ccaaaataat ttcgcaaaat atccgagaat attttggaaa gtctttgcca
1381 gttgatctaa cgtgtaatca ttttggtatt gctcgcatac caagaacgga caatgtagaa
1441 ttttttgatc ctaattaccg ttattctttc aaagaatggc aagattggtc tttcaaacaa
1501 acagataata agggctttac tcgttcaagt ctaacggttt taagcggtac agaaggcaaa
1561 aaacaagtag atgaaccctg gtttaatctc ttattgcacg aaacgaaatt ttcaggagaa
1621 aagggtttaa tagggcgtaa taacgtcatg tttaccctct ctttagccta ctttagttca
1681 ggctattcaa tcgaaacgtg cgaatataat atgtttgagt ttaataatcg attagatcaa
1741 cccttagaag aaaaagaagt aatcaaaatt gttagaagtg cctattcaga aaactatcaa
1801 ggggctaata gggaatacat taccattctt tgcaaagctt gggtatcaag tgatttaacc
1861 agtaaagatt tatttgtccg tcaagggtgg tttaaattca agaaaaaaag aagcgaacgt
1921 caacgtgttc atttgtcaga atggaaagaa gatttaatgg cttatattag cgaaaaaagc
1981 gatgtataca agccttattt agtgacgacc aaaaaagaga ttagagaagt gctaggcatt
2041 cctgaacgga cattagataa attgctgaag gtactgaagg cgaatcagga aattttcttt
2101 aagattaaac caggaagaaa tggtggcatt caacttgcta gtgttaaatc attgttgcta
2161 tcgatcatta aagtaaaaaa agaagaaaaa gaaagctata taaaggcgct gacaaattct
2221 tttgacttag agcatacatt cattcaagag actttaaaca agctagcaga acgccctaaa
2281 acggacacac aactcgattt gtttagctat gatacaggct gaaaataaaa cccgcactat
2341 gccattacat ttatatctat gatacgtgtt tgttttttct ttgctgttta gcgaatgatt
2401 agcagaaata tacagagtaa gattttaatt aattattagg gggagaagga gagagtagcc
2461 cgaaaacttt tagttggctt ggactgaacg aagtgaggga aaggctacta aaacgtcgag
2521 gggcagtgag agcgaagcga acacttgatt ttttaatttt ctatctttta taggtcatta
2581 gagtatactt atttgtccta taaactattt agcagcataa tagatttatt gaataggtca
2641 tttaagttga gcatattaga ggaggaaaat cttggagaaa tatttgaaga acccgattac
2701 atggattgga ttagttcttg tggttacgtg gtttttaact aaaagtagtg aatttttgat
2761 ttttggtgtg tgtgtcttgt tgttagtatt tgctagtcaa agtgattaaa tagaattcac
2821 tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc
2881 ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc accgatcgcc
2941 cttcccaaca gttgcgcagc ctgaatggcg aatggcgcct gatgcggtat tttctcctta
3001 cgcatctgtg cggtatttca caccgcatat ggtgcactct cagtacaatc tgctctgatg
3061 ccgcatagtt aagccagccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt
3121 gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc
3181 agaggttttc accgtcatca ccgaaacgcg cgagacgaaa gggcctcgtg atacgcctat
3241 ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg
3301 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
3361 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta
3421 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg
3481 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg
3541 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac
3601 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg
3661 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt
3721 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg
3781 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac
3841 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt
3901 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag
3961 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc
4021 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc
4081 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta
4141 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg
4201 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga
4261 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac
4321 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
4381 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
4441 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
4501 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
4561 gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc
4621 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
4681 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
4741 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
4801 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
4861 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
4921 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
4981 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
5041 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
5101 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg
5161 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc
5221 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
5281 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
5341 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
5401 gcggataaca atttcacaca ggaaacagct atgaccatga ttacgccaag ctcgaaatta
5461 accctcacta aagggaacaa aagctgggta ccgggccccc cctcgacggt atcgatagcc
5521 cgcctaatga gcgggctttt ttttgatccc ccaaagaagc gcgtaatatc aggtttaaga
5581 gacaataaga aaaaaagatt gaaaaaagtg acattaaatt cttgacaggg agagataggt
5641 ttgatagaat ataataggtt tgatagaata taatagggta ccgagctcga attcctgcag
5701 cccaaatcgc gaaaccgaac ttaatgggag gaaaaattaa aaaagaacag ttatgaaaaa
5761 aaagattatc tcagctattt taatgtctac agtgatactt tctgctgcag ccccgttgtc
5821 aggtgtttac gctgacacaa actcagatat tgctaaacaa gatgcggtcg acccattgtc
5881 aactaaaaaa ttacataaag aacctgcgac tttaattaaa gcgattgatg gtgatacggt
5941 taaattaatg tacaaaggtc aaccaatgac attcagacta ttattggttg atacacctga
6001 aacaaagcat cctaaaaaag gtgtagagaa atatggtcct gaagcaagtg catttacgaa
6061 aaaaatggta gaaaatgcaa agaaaattga agtcgagttt gacaaaggtc aaagaactga
6121 taaatatgga cgtggcttag cgtatattta tgctgatgga aaaatggtaa acgaagcttt
6181 agttcgtcaa ggcttggcta aagttgctta tgtttacaaa cctaacaata cacatgaaca
6241 acatttaaga aaaagtgaag cacaagcgaa aaaagagaaa ttaaatattt ggagcgaaga
6301 caacgctgat tcaggtcaaa tcgatctcga gatatcgcta gttctagatt gaggcatcaa
6361 ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg
6421 aacgctctcc tgagtaggac aaatccgccg ctctagctaa gcagaaggcc atcctgacgg
6481 atggcctttt tgcgtttcta caaactcttg ttggggatcc gcctgcagcc aagcttatcg
6541 attctagact cgagagctct cgagtctaga atcgatacga ttttgaagtg gcaacagata
6601 aaaaaaagca gtttaaaatt gttgctgaac ttttaaaaca agcaaataca atcattgtcg
6661 caacagatag cgacagagaa ggcgaaaaca ttgcctggtc gatcattcat aaagcaaatg
6721 ccttttctaa agataaaacg tataaaagac tatggatcaa tagtttagaa aaagatgtga
6781 tccgtagcgg ttttcaaaat ttgcaaccag gaatgaatta ctatcccttt tatcaagaag
6841 cgcaaaagaa aaacgaaatg atacaccaat cagtgcaaaa aaagatataa tgggagataa
6901 gacggttcgt gttcgtgctg acttgcacca tatcataaaa atcgaaacag caaagaatgg
6961 cggaaacgta aaagaagtta tggaaataag acttagaagc aaacttaaga gtgtgttgat
7021 agtgcagtat cttaaaattt tgtataatag gaattgaagt taaattagat gctaaaaatt
7081 tgtaattaag aaggagtgat tacatgaaca aaaatataaa atattctcaa aactttttaa
7141 cgagtgaaaa agtactcaac caaataataa aacaattgaa tttaaaagaa accgataccg
7201 tttacgaaat tggaacaggt aaagggcatt taacgacgaa actggctaaa ataagtaaac
7261 aggtaacgtc tattgaatta gacagtcatc tattcaactt atcgtcagaa aaattaaaac
7321 tgaatactcg tgtcacttta attcaccaag atattctaca gtttcaattc cctaacaaac
7381 agaggtataa aattgttggg agtattcctt accatttaag cacacaaatt attaaaaaag
7441 tggtttttga aagccatgcg tctgacatct atctgattgt tgaagaagga ttctacaagc
7501 gtaccttgga tattcaccga acactagggt tgctcttgca cactcaagtc tcgattcagc
7561 aattgcttaa gctgccagcg gaatgctttc atcctaaacc aaaagtaaac agtgtcttaa
7621 taaaacttac ccgccatacc acagatgttc cagataaata ttggaagcta tatacgtact
7681 ttgtttcaaa atgggtcaat cgagaatatc gtcaactgtt tactaaaaat cagtttcatc
7741 aagcaatgaa acacgccaaa gtaaacaatt taagtaccgt tacttatgag caagtattgt
7801 ctatttttaa tagttatcta ttatttaacg ggaggaaata attctatgag tcgcttttgt
7861 aaatttggaa agttacacgt tactaaaggg aatgtagata aattattagg tatactactg
7921 acagcttcca aggagctaaa gaggtcccta gcgcttagaa tcgctttagg aaacacgatc
7981 cagtccaata atcgtcgata aaaacttttg aaaaaggttg gtgaaattac ctacttttgg
8041 aataatcaca aatcacaagt gattaatcac aaatcacaag tgattaatca cttgtttatt
8101 aagatattaa aagctataat ttaaataaag cgtgaatttt attacacaaa aagagggggg
8161 agaaacttgg aactagcatt tagagaaagc ttaaaaaaga tgagaggtac caaatcaaaa
8221 gaaaaattct cccaagaatt agaaatgagt agatcaaatt attcacgaat agaatcagga
8281 aaatcagatc caaccataaa aacactagaa caaattgcaa agttaact
//