LOCUS Exported 3610 bp ds-DNA circular SYN 14-JUL-2016
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS pMG36e
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 3610)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Thursday, July 14, 2016 from SnapGene Viewer 3.1.4
http://www.miaolingbio.com
FEATURES Location/Qualifiers
source 1..3610
/organism="synthetic DNA construct"
/mol_type="other DNA"
CDS 227..925
/codon_start=1
/note="repA"
/translation="MAIKNTKARNFGFLLYPDSIPNDWKEKLESLGVSMAVSPLHDMDE
KKDKDTWNSSDVIRNGKHYKKPHYHVIYIARNPVTIESVRNKIKRKLGNSSVAHVEILD
YIKGSYEYLTHESKDAIAKNKHIYDKKDILNINDFDIDRYITLDESQKRELKNLLLDIV
DDYNLVNTKDLMAFIRLRGAEFGILNTNDVKDIVSTNSSAFRLWFEGNYQCGYRASYAK
VLDAETGEIK"
CDS complement(1463..2197)
/codon_start=1
/note="Emr"
/translation="MNEKNIKHSQNFITSKHNIDKIMTNIRLNEHDNIFEIGSGKGHFT
LELVKRCNFVTAIEIDHKLCKTTENKLVDHDNFQVLNKDILQFKFPKNQSYKIYGNIPY
NISTDIIRKIVFDSIANEIYLIVEYGFAKRLLNTKRSLALLLMAEVDISILSMVPREYF
HPKPKVNSSLIRLSRKKSRISHKDKQKYNYFVMKWVNKEYKKIFTKNQFNNSLKHAGID
DLNNISFEQFLSLFNSYKLFNK"
misc_feature 2474..2652
/note="P32"
CDS 2653..2892
/codon_start=1
/note="ORF"
/translation="MAIVSAEKFVIRARPGIDPLESTCRHASLQSLKTKVAAAVEAAKT
VGKGDGTTGTSDKGGGQGTPALRYLELRFKVKWY"
CDS complement(3215..3226)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/note="Factor Xa site"
/translation="IEGR"
ORIGIN
1 ggtgatttca gaatcgaaaa aaagagttat gatttctctg acaaaagagc aagataaaaa
61 attaacagat atggcgaaac aaaaaggttt ttcaaaatct gcggttgcgg cgttagctat
121 agaagaatat gcaagaaagg aatcagaaca aaaaaaataa gcgaaagctc gcgtttttag
181 aaggatacga gttttcgcta cttgtttttg ataaggtaat tatatcatgg ctattaaaaa
241 tactaaagct agaaattttg gatttttatt atatcctgac tcaattccta atgattggaa
301 agaaaaatta gagagtttgg gcgtatctat ggctgtcagt cctttacacg atatggacga
361 aaaaaaagat aaagatacat ggaatagtag tgatgttata cgaaatggaa agcactataa
421 aaaaccacac tatcacgtta tatatattgc acgaaatcct gtaacaatag aaagcgttag
481 gaacaagatt aagcgaaaat tggggaatag ttcagttgct catgttgaga tacttgatta
541 tatcaaaggt tcatatgaat atttgactca tgaatcaaag gacgctattg ctaagaataa
601 acatatatac gacaaaaaag atattttgaa cattaatgat tttgatattg accgctatat
661 aacacttgat gaaagccaaa aaagagaatt gaagaattta cttttagata tagtggatga
721 ctataatttg gtaaatacaa aagatttaat ggcttttatt cgccttaggg gagcggagtt
781 tggaatttta aatacgaatg atgtaaaaga tattgtttca acaaactcta gcgcctttag
841 attatggttt gagggcaatt atcagtgtgg atatagagca agttatgcaa aggttcttga
901 tgctgaaacg ggggaaataa aatgacaaac aaagaaaaag agttatttgc tgaaaatgag
961 gaattaaaaa aagaaattaa ggacttaaaa gagcgtattg aaagatacag agaaatggaa
1021 gttgaattaa gtacaacaat agatttattg agaggaggga ttattgaata aataaaagcc
1081 ccctgacgaa agtcgaaggg ggtttttatt ttggtttgat gttgcgatta atagcaatac
1141 aattgcaata aacaaaatga tcgacctcgg gacccctatc tagcgaactt ttagaaaaga
1201 tataaaacat cagagtatgg acagttgcgg atgtacttca gaaaagatta gatgtctaaa
1261 aagctagctt tttagacatc taaatctagg tactaaaaca attcatccag taaaatataa
1321 tattttattt tctcccaatc aggcttgatc cccagtaagt caaaaaatag ctcgacatac
1381 tgttcttccc cgatcgaccc gattcacaaa aaataggcac acgaaaaaca agttaaggga
1441 tgcagtttat gcatccctta acttacttat taaataattt atagctattg aaaagagata
1501 agaattgttc aaagctaata ttgtttaaat cgtcaattcc tgcatgtttt aaggaattgt
1561 taaattgatt ttttgtaaat attttcttgt attctttgtt aacccatttc ataacgaaat
1621 aattatactt ttgtttatct ttgtgtgata ttcttgattt ttttctactt aatctgataa
1681 gtgagctatt cactttaggt ttaggatgaa aatattctct tggaaccata cttaatatag
1741 aaatatcaac ttctgccatt aaaagtaatg ccaatgagcg ttttgtattt aataatcttt
1801 tagcaaaccc gtattccacg attaaataaa tctcattagc tatactatca aaaacaattt
1861 tgcgtattat atccgtactt atgttataag gtatattacc atatatttta taggattggt
1921 ttttaggaaa tttaaactgc aatatatcct tgtttaaaac ttggaaatta tcgtgatcaa
1981 caagtttatt ttctgtagtt ttgcataatt tatggtctat ttcaatggca gttacgaaat
2041 tacacctctt tactaattca agggtaaaat ggccttttcc tgagccgatt tcaaagatat
2101 tatcatgttc atttaatctt atatttgtca ttattttatc tatattatgt tttgaagtaa
2161 taaagttttg actgtgtttt atatttttct cgttcattat aaccctcttt aatttggtta
2221 tatgaatttt gcttattaac gattcattat aaccacttat tttttgtttg gttgataatg
2281 aactgtgctg attacaaaaa tactaaaaat gcccatattt tttcctcctt ataaaattag
2341 tataattata gcacggtcga tcttctatat aaaagatata ttatcttatc agtattgtca
2401 atatattcaa ggcaatctgc ctcctcatcc tcttcatcct cttcgtcttg gtagcttttt
2461 aaatatgggt cgatcgaatt cggtcctcgg gatatgataa gattaatagt tttagctatt
2521 aatctttttt tatttttatt taagaatggc ttaataaagc ggttactttg gatttttgtg
2581 agcttggact agaaaaaaac ttcacaaaat gctatactag gtaggtaaaa aaatattcgg
2641 aggaattttg aaatggcaat cgtttcagca gaaaaattcg taattcgagc tcgcccgggg
2701 atcgatcctc tagagtcgac ctgcaggcat gcaagcttgc aaagtctgaa aacgaaggtg
2761 gcagctgccg ttgaagcggc caagacagtt ggtaaaggcg acggtacaac cggtactagc
2821 gacaaaggcg gcggtcaagg taccccggcg ctacgatatt tggagttgag gttcaaagtc
2881 aaatggtact gatgaccggt aaaatttaat attttgaacc ttgcttaggc agctgacttc
2941 acattgttga gatcagctgc cttttgctta tagttcattg agtagaaacg gttctgttgc
3001 gaagtttgaa aatcaaacgc aagctcgatt ttttattaaa acgtctcaaa atcgtttctg
3061 agacgtttta gcgtttattt cgtttagtta tcggcataat cgttaaaaca ggcgttatcg
3121 tagcgtaaaa gcccttgagc gtagcgtggc tttgcagcga agatgttgtc tgttagatta
3181 tgaaagccga tgactgaatg aaataataag cgcagcgccc ttctatttcg gttggaggag
3241 gctcaaggga gtatgaggga atgaaattcc ctcatgggtt tgattttaaa aattgcttgc
3301 aattttgccg agcggtagcg ctggaaaatt tttgaaaaaa atttggaatt tggaaaaaaa
3361 tggggggaaa ggaagcgaat tttgcttccg tactacgacc ccccattaag tgccgagtgc
3421 caatttttgt gccaaaaacg ctctatccca actggctcaa gggtttaagg ggtttttcaa
3481 tcgccaacga atcgccaacg ttttcgccaa cgttttttat aaatctatat ttaagtagct
3541 ttattgttgt ttttatgatt acaaagtgat acactaactt tataaaatta tttgattgga
3601 gttttttaat
//