LOCUS Exported 12765 bp ds-DNA circular SYN 22-SEP-2016
DEFINITION synthetic circular DNA
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 12765)
AUTHORS .
TITLE Direct Submission
http://www.miaolingbio.com
FEATURES Location/Qualifiers
source 1..12765
/organism="synthetic DNA construct"
/mol_type="other DNA"
CDS 14..1819
/codon_start=1
/gene="uidA (or gusA) from E. coli"
/product="beta-glucuronidase"
/label=GUS
/translation="LRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESALQESRAIA
VPGSFNDQFADADIRNYAGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQEVM
EHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSYFHDF
FNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVSVELRDADQQV
VATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAVKGQQFL
INHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYAEEMLDWA
DEHGIVVIDETAAVGFQLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAIKELIARDK
NHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCDAHTDTISDL
FDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVDTLAGLHSMYT
DMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGNKKGIFTRDRKP
KSAAFLLQKRWTGMNFGEKPQQGGKQ"
CDS 1823..2533
/codon_start=1
/gene="mgfp5"
/product="GFP with folding enhancement mutations"
/label=mgfp5
/note="suitable for expression in plants due to removal of
a cryptic intron"
/translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF
ICTTGKLPVPWPTLVTTFSYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDGN
YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKAN
FKTRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEF
VTAAGITHGMDELYK"
CDS 2540..2557
/codon_start=1
/product="6xHis affinity tag"
/label=6xHis
/translation="HHHHHH"
terminator 2589..2841
/label=NOS terminator
/note="nopaline synthase terminator and poly(A) signal"
misc_feature 2863..2887
/label=RB T-DNA repeat
/note="right border repeat from nopaline C58 T-DNA"
CDS 4187..4816
/codon_start=1
/product="stability protein from the Pseudomonas plasmid
pVS1 (Heeb et al., 2000)"
/label=pVS1 StaA
/translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
CDS 5250..6317
/codon_start=1
/product="replication protein from the Pseudomonas plasmid
pVS1 (Heeb et al., 2000)"
/label=pVS1 RepA
/translation="GRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQA
AADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKR
DRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGR
VFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEAL
ISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLA
RRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILV
MRYRNLIEGEASAGS"
rep_origin 6383..6577
/label=pVS1 oriV
/note="origin of replication for the Pseudomonas plasmid
pVS1 (Heeb et al., 2000)"
misc_feature 6921..7061
/label=bom
/note="basis of mobility region from pBR322"
rep_origin complement(7247..7835)
/direction=LEFT
/label=pUC ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(7922..8716)
/codon_start=1
/gene="aphA-3"
/product="aminoglycoside phosphotransferase"
/label=KanR
/note="confers resistance to kanamycin"
/translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
misc_feature 9141..9165
/label=LB T-DNA repeat
/note="left border repeat from nopaline C58 T-DNA"
polyA_signal 9243..9417
/label=CaMV poly(A) signal
/note="cauliflower mosaic virus polyadenylation signal"
misc_feature 9424..9654
/label=TrpC terminator
CDS complement(9740..10765)
/codon_start=1
/gene="aph(4)-Ia"
/product="aminoglycoside phosphotransferase from E. coli"
/label=HygR
/note="confers resistance to hygromycin"
/translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
K"
misc_feature complement(10766..11151)
/label=TrpC promoter
promoter complement(11242..11343)
/gene="S. cerevisiae TRP1"
/label=TRP1 promoter
misc_feature 11356..12765
/label=gpdA
ORIGIN
1 agatctgact agtttacgtc ctgtagaaac cccaacccgt gaaatcaaaa aactcgacgg
61 cctgtgggca ttcagtctgg atcgcgaaaa ctgtggaatt gatcagcgtt ggtgggaaag
121 cgcgttacaa gaaagccggg caattgctgt gccaggcagt tttaacgatc agttcgccga
181 tgcagatatt cgtaattatg cgggcaacgt ctggtatcag cgcgaagtct ttataccgaa
241 aggttgggca ggccagcgta tcgtgctgcg tttcgatgcg gtcactcatt acggcaaagt
301 gtgggtcaat aatcaggaag tgatggagca tcagggcggc tatacgccat ttgaagccga
361 tgtcacgccg tatgttattg ccgggaaaag tgtacgtatc accgtttgtg tgaacaacga
421 actgaactgg cagactatcc cgccgggaat ggtgattacc gacgaaaacg gcaagaaaaa
481 gcagtcttac ttccatgatt tctttaacta tgccggaatc catcgcagcg taatgctcta
541 caccacgccg aacacctggg tggacgatat caccgtggtg acgcatgtcg cgcaagactg
601 taaccacgcg tctgttgact ggcaggtggt ggccaatggt gatgtcagcg ttgaactgcg
661 tgatgcggat caacaggtgg ttgcaactgg acaaggcact agcgggactt tgcaagtggt
721 gaatccgcac ctctggcaac cgggtgaagg ttatctctat gaactgtgcg tcacagccaa
781 aagccagaca gagtgtgata tctacccgct tcgcgtcggc atccggtcag tggcagtgaa
841 gggccaacag ttcctgatta accacaaacc gttctacttt actggctttg gtcgtcatga
901 agatgcggac ttacgtggca aaggattcga taacgtgctg atggtgcacg accacgcatt
961 aatggactgg attggggcca actcctaccg tacctcgcat tacccttacg ctgaagagat
1021 gctcgactgg gcagatgaac atggcatcgt ggtgattgat gaaactgctg ctgtcggctt
1081 tcagctgtct ttaggcattg gtttcgaagc gggcaacaag ccgaaagaac tgtacagcga
1141 agaggcagtc aacggggaaa ctcagcaagc gcacttacag gcgattaaag agctgatagc
1201 gcgtgacaaa aaccacccaa gcgtggtgat gtggagtatt gccaacgaac cggatacccg
1261 tccgcaaggt gcacgggaat atttcgcgcc actggcggaa gcaacgcgta aactcgaccc
1321 gacgcgtccg atcacctgcg tcaatgtaat gttctgcgac gctcacaccg ataccatcag
1381 cgatctcttt gatgtgctgt gcctgaaccg ttattacgga tggtatgtcc aaagcggcga
1441 tttggaaacg gcagagaagg tactggaaaa agaacttctg gcctggcagg agaaactgca
1501 tcagccgatt atcatcaccg aatacggcgt ggatacgtta gccgggctgc actcaatgta
1561 caccgacatg tggagtgaag agtatcagtg tgcatggctg gatatgtatc accgcgtctt
1621 tgatcgcgtc agcgccgtcg tcggtgaaca ggtatggaat ttcgccgatt ttgcgacctc
1681 gcaaggcata ttgcgcgttg gcggtaacaa gaaagggatc ttcactcgcg accgcaaacc
1741 gaagtcggcg gcttttctgc tgcaaaaacg ctggactggc atgaacttcg gtgaaaaacc
1801 gcagcaggga ggcaaacaag ctagtaaagg agaagaactt ttcactggag ttgtcccaat
1861 tcttgttgaa ttagatggtg atgttaatgg gcacaaattt tctgtcagtg gagagggtga
1921 aggtgatgca acatacggaa aacttaccct taaatttatt tgcactactg gaaaactacc
1981 tgttccgtgg ccaacacttg tcactacttt ctcttatggt gttcaatgct tttcaagata
2041 cccagatcat atgaagcggc acgacttctt caagagcgcc atgcctgagg gatacgtgca
2101 ggagaggacc atcttcttca aggacgacgg gaactacaag acacgtgctg aagtcaagtt
2161 tgagggagac accctcgtca acaggatcga gcttaaggga atcgatttca aggaggacgg
2221 aaacatcctc ggccacaagt tggaatacaa ctacaactcc cacaacgtat acatcatggc
2281 cgacaagcaa aagaacggca tcaaagccaa cttcaagacc cgccacaaca tcgaagacgg
2341 cggcgtgcaa ctcgctgatc attatcaaca aaatactcca attggcgatg gccctgtcct
2401 tttaccagac aaccattacc tgtccacaca atctgccctt tcgaaagatc ccaacgaaaa
2461 gagagaccac atggtccttc ttgagtttgt aacagctgct gggattacac atggcatgga
2521 tgaactatac aaagctagcc accaccacca ccaccacgtg tgaattggtg accagctcga
2581 atttccccga tcgttcaaac atttggcaat aaagtttctt aagattgaat cctgttgccg
2641 gtcttgcgat gattatcata taatttctgt tgaattacgt taagcatgta ataattaaca
2701 tgtaatgcat gacgttattt atgagatggg tttttatgat tagagtcccg caattataca
2761 tttaatacgc gatagaaaac aaaatatagc gcgcaaacta ggataaatta tcgcgcgcgg
2821 tgtcatctat gttactagat cgggaattaa actatcagtg tttgacagga tatattggcg
2881 ggtaaaccta agagaaaaga gcgtttatta gaataacgga tatttaaaag ggcgtgaaaa
2941 ggtttatccg ttcgtccatt tgtatgtgca tgccaaccac agggttcccc tcgggatcaa
3001 agtactttga tccaacccct ccgctgctat agtgcagtcg gcttctgacg ttcagtgcag
3061 ccgtcttctg aaaacgacat gtcgcacaag tcctaagtta cgcgacaggc tgccgccctg
3121 cccttttcct ggcgttttct tgtcgcgtgt tttagtcgca taaagtagaa tacttgcgac
3181 tagaaccgga gacattacgc catgaacaag agcgccgccg ctggcctgct gggctatgcc
3241 cgcgtcagca ccgacgacca ggacttgacc aaccaacggg ccgaactgca cgcggccggc
3301 tgcaccaagc tgttttccga gaagatcacc ggcaccaggc gcgaccgccc ggagctggcc
3361 aggatgcttg accacctacg ccctggcgac gttgtgacag tgaccaggct agaccgcctg
3421 gcccgcagca cccgcgacct actggacatt gccgagcgca tccaggaggc cggcgcgggc
3481 ctgcgtagcc tggcagagcc gtgggccgac accaccacgc cggccggccg catggtgttg
3541 accgtgttcg ccggcattgc cgagttcgag cgttccctaa tcatcgaccg cacccggagc
3601 gggcgcgagg ccgccaaggc ccgaggcgtg aagtttggcc cccgccctac cctcaccccg
3661 gcacagatcg cgcacgcccg cgagctgatc gaccaggaag gccgcaccgt gaaagaggcg
3721 gctgcactgc ttggcgtgca tcgctcgacc ctgtaccgcg cacttgagcg cagcgaggaa
3781 gtgacgccca ccgaggccag gcggcgcggt gccttccgtg aggacgcatt gaccgaggcc
3841 gacgccctgg cggccgccga gaatgaacgc caagaggaac aagcatgaaa ccgcaccagg
3901 acggccagga cgaaccgttt ttcattaccg aagagatcga ggcggagatg atcgcggccg
3961 ggtacgtgtt cgagccgccc gcgcacgtct caaccgtgcg gctgcatgaa atcctggccg
4021 gtttgtctga tgccaagctg gcggcctggc cggccagctt ggccgctgaa gaaaccgagc
4081 gccgccgtct aaaaaggtga tgtgtatttg agtaaaacag cttgcgtcat gcggtcgctg
4141 cgtatatgat gcgatgagta aataaacaaa tacgcaaggg gaacgcatga aggttatcgc
4201 tgtacttaac cagaaaggcg ggtcaggcaa gacgaccatc gcaacccatc tagcccgcgc
4261 cctgcaactc gccggggccg atgttctgtt agtcgattcc gatccccagg gcagtgcccg
4321 cgattgggcg gccgtgcggg aagatcaacc gctaaccgtt gtcggcatcg accgcccgac
4381 gattgaccgc gacgtgaagg ccatcggccg gcgcgacttc gtagtgatcg acggagcgcc
4441 ccaggcggcg gacttggctg tgtccgcgat caaggcagcc gacttcgtgc tgattccggt
4501 gcagccaagc ccttacgaca tatgggccac cgccgacctg gtggagctgg ttaagcagcg
4561 cattgaggtc acggatggaa ggctacaagc ggcctttgtc gtgtcgcggg cgatcaaagg
4621 cacgcgcatc ggcggtgagg ttgccgaggc gctggccggg tacgagctgc ccattcttga
4681 gtcccgtatc acgcagcgcg tgagctaccc aggcactgcc gccgccggca caaccgttct
4741 tgaatcagaa cccgagggcg acgctgcccg cgaggtccag gcgctggccg ctgaaattaa
4801 atcaaaactc atttgagtta atgaggtaaa gagaaaatga gcaaaagcac aaacacgcta
4861 agtgccggcc gtccgagcgc acgcagcagc aaggctgcaa cgttggccag cctggcagac
4921 acgccagcca tgaagcgggt caactttcag ttgccggcgg aggatcacac caagctgaag
4981 atgtacgcgg tacgccaagg caagaccatt accgagctgc tatctgaata catcgcgcag
5041 ctaccagagt aaatgagcaa atgaataaat gagtagatga attttagcgg ctaaaggagg
5101 cggcatggaa aatcaagaac aaccaggcac cgacgccgtg gaatgcccca tgtgtggagg
5161 aacgggcggt tggccaggcg taagcggctg ggttgtctgc cggccctgca atggcactgg
5221 aacccccaag cccgaggaat cggcgtgacg gtcgcaaacc atccggcccg gtacaaatcg
5281 gcgcggcgct gggtgatgac ctggtggaga agttgaaggc cgcgcaggcc gcccagcggc
5341 aacgcatcga ggcagaagca cgccccggtg aatcgtggca agcggccgct gatcgaatcc
5401 gcaaagaatc ccggcaaccg ccggcagccg gtgcgccgtc gattaggaag ccgcccaagg
5461 gcgacgagca accagatttt ttcgttccga tgctctatga cgtgggcacc cgcgatagtc
5521 gcagcatcat ggacgtggcc gttttccgtc tgtcgaagcg tgaccgacga gctggcgagg
5581 tgatccgcta cgagcttcca gacgggcacg tagaggtttc cgcagggccg gccggcatgg
5641 ccagtgtgtg ggattacgac ctggtactga tggcggtttc ccatctaacc gaatccatga
5701 accgataccg ggaagggaag ggagacaagc ccggccgcgt gttccgtcca cacgttgcgg
5761 acgtactcaa gttctgccgg cgagccgatg gcggaaagca gaaagacgac ctggtagaaa
5821 cctgcattcg gttaaacacc acgcacgttg ccatgcagcg tacgaagaag gccaagaacg
5881 gccgcctggt gacggtatcc gagggtgaag ccttgattag ccgctacaag atcgtaaaga
5941 gcgaaaccgg gcggccggag tacatcgaga tcgagctagc tgattggatg taccgcgaga
6001 tcacagaagg caagaacccg gacgtgctga cggttcaccc cgattacttt ttgatcgatc
6061 ccggcatcgg ccgttttctc taccgcctgg cacgccgcgc cgcaggcaag gcagaagcca
6121 gatggttgtt caagacgatc tacgaacgca gtggcagcgc cggagagttc aagaagttct
6181 gtttcaccgt gcgcaagctg atcgggtcaa atgacctgcc ggagtacgat ttgaaggagg
6241 aggcggggca ggctggcccg atcctagtca tgcgctaccg caacctgatc gagggcgaag
6301 catccgccgg ttcctaatgt acggagcaga tgctagggca aattgcccta gcaggggaaa
6361 aaggtcgaaa aggtctcttt cctgtggata gcacgtacat tgggaaccca aagccgtaca
6421 ttgggaaccg gaacccgtac attgggaacc caaagccgta cattgggaac cggtcacaca
6481 tgtaagtgac tgatataaaa gagaaaaaag gcgatttttc cgcctaaaac tctttaaaac
6541 ttattaaaac tcttaaaacc cgcctggcct gtgcataact gtctggccag cgcacagccg
6601 aagagctgca aaaagcgcct acccttcggt cgctgcgctc cctacgcccc gccgcttcgc
6661 gtcggcctat cgcggccgct ggccgctcaa aaatggctgg cctacggcca ggcaatctac
6721 cagggcgcgg acaagccgcg ccgtcgccac tcgaccgccg gcgcccacat caaggcaccc
6781 tgcctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct cccggagacg
6841 gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg
6901 ggtgttggcg ggtgtcgggg cgcagccatg acccagtcac gtagcgatag cggagtgtat
6961 actggcttaa ctatgcggca tcagagcaga ttgtactgag agtgcaccat atgcggtgtg
7021 aaataccgca cagatgcgta aggagaaaat accgcatcag gcgctcttcc gcttcctcgc
7081 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
7141 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
7201 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
7261 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
7321 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
7381 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
7441 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
7501 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
7561 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
7621 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
7681 ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
7741 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
7801 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
7861 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg cattctaggt
7921 actaaaacaa ttcatccagt aaaatataat attttatttt ctcccaatca ggcttgatcc
7981 ccagtaagtc aaaaaatagc tcgacatact gttcttcccc gatatcctcc ctgatcgacc
8041 ggacgcagaa ggcaatgtca taccacttgt ccgccctgcc gcttctccca agatcaataa
8101 agccacttac tttgccatct ttcacaaaga tgttgctgtc tcccaggtcg ccgtgggaaa
8161 agacaagttc ctcttcgggc ttttccgtct ttaaaaaatc atacagctcg cgcggatctt
8221 taaatggagt gtcttcttcc cagttttcgc aatccacatc ggccagatcg ttattcagta
8281 agtaatccaa ttcggctaag cggctgtcta agctattcgt atagggacaa tccgatatgt
8341 cgatggagtg aaagagcctg atgcactccg catacagctc gataatcttt tcagggcttt
8401 gttcatcttc atactcttcc gagcaaagga cgccatcggc ctcactcatg agcagattgc
8461 tccagccatc atgccgttca aagtgcagga cctttggaac aggcagcttt ccttccagcc
8521 atagcatcat gtccttttcc cgttccacat cataggtggt ccctttatac cggctgtccg
8581 tcatttttaa atataggttt tcattttctc ccaccagctt atatacctta gcaggagaca
8641 ttccttccgt atcttttacg cagcggtatt tttcgatcag ttttttcaat tccggtgata
8701 ttctcatttt agccatttat tatttccttc ctcttttcta cagtatttaa agatacccca
8761 agaagctaat tataacaaga cgaactccaa ttcactgttc cttgcattct aaaaccttaa
8821 ataccagaaa acagcttttt caaagttgtt ttcaaagttg gcgtataaca tagtatcgac
8881 ggagccgatt ttgaaaccgc ggtgatcaca ggcagcaacg ctctgtcatc gttacaatca
8941 acatgctacc ctccgcgaga tcatccgtgt ttcaaacccg gcagcttagt tgccgttctt
9001 ccgaatagca tcggtaacat gagcaaagtc tgccgcctta caacggctct cccgctgacg
9061 ccgtcccgga ctgatgggct gcctgtatcg agtggtgatt ttgtgccgag ctgccggtcg
9121 gggagctgtt ggctggctgg tggcaggata tattgtggtg taaacaaatt gacgcttaga
9181 caacttaata acacattgcg gacgttttta atgtactgaa ttaacgccga attaattcgg
9241 gggatctgga ttttagtact ggattttggt tttaggaatt agaaatttta ttgatagaag
9301 tattttacaa atacaaatac atactaaggg tttcttatat gctcaacaca tgagcgaaac
9361 cctataggaa ccctaattcc cttatctggg aactactcac acattattat ggagaaactc
9421 gaggcattgc agatgagctg tatctggaag aggtaaaccc gaaacgcgtt ttattcttgt
9481 tgacatggag ctattaaatc actagaaggc actctttgct gcttggacaa atgaacgtat
9541 cttatcgaga tcctgaacac catttgtctc aactccggag ctgacatcga caccaacgat
9601 cttatatcca gattcgtcaa gctgtttgat gatttcagta acgttaagtg gatcatgata
9661 atcatcgcaa gaccggcaac aggattcaat cttaagaaac tttattgcca aatgtttgaa
9721 cgatctgcag gtcgacggac tatttctttg ccctcggacg agtgctgggg cgtcggtttc
9781 cactatcggc gagtacttct acacagccat cggtccagac ggccgcgctt ctgcgggcga
9841 tttgtgtacg cccgacagtc ccggctccgg atcggacgat tgcgtcgcat cgaccctgcg
9901 cccaagctgc atcatcgaaa ttgccgtcaa ccaagctctg atagagttgg tcaagaccaa
9961 tgcggagcat atacgcccgg agtcgtggcg atcctgcaag ctccggatgc ctccgctcga
10021 agtagcgcgt ctgctgctcc atacaagcca accacggcct ccagaagaag atgttggcga
10081 cctcgtattg ggaatccccg aacatcgcct cgctccagtc aatgaccgct gttatgcggc
10141 cattgtccgt caggacattg ttggagccga aatccgcgtg cacgaggtgc cggacttcgg
10201 ggcagtcctc ggcccaaagc atcagctcat cgagagcctg cgcgacggac gcactgacgg
10261 tgtcgtccat cacagtttgc cagtgataca catggggatc agcaatcgcg catatgaaat
10321 cacgccatgt agtgtattga ccgattcctt gcggtccgaa tgggccgaac ccgctcgtct
10381 ggctaagatc ggccgcagcg atcgcatcca tagcctccgc gaccggttgt agaacagcgg
10441 gcagttcggt ttcaggcagg tcttgcaacg tgacaccctg tgcacggcgg gagatgcaat
10501 aggtcaggct ctcgctaaac tccccaatgt caagcacttc cggaatcggg agcgcggccg
10561 atgcaaagtg ccgataaaca taacgatctt tgtagaaacc atcggcgcag ctatttaccc
10621 gcaggacata tccacgccct cctacatcga agctgaaagc acgagattct tcgccctccg
10681 agagctgcat caggtcggag acgctgtcga acttttcgat cagaaacttc tcgacagacg
10741 tcgcggtgag ttcaggcttt ttcatggtta cttcctaatc gaagctttgc ttgggtagaa
10801 taggtaagtc agattgaatc tgaaataaag ggaggaaggg cgaacttaag aaggtatgac
10861 cgggtcgttc acttaccttg cttgacaaac gcaccaagtt atcgtgcacc aagcagcaga
10921 tgataataat gtcctcgttc ctgtctgcta ataagagtca cacttcgagc gccgccgcta
10981 ctgctacaag tggggctgat ctgaccagtt gcctaaatga accatcttgt caaacgacac
11041 aaattttgtg ctcaccgcct ggacgactaa accaaaatag gcattcattg ttgacctcca
11101 ctagctccag ccaagcccaa aaagtgctcc ttcaatatca tcttctgtcg agtctagagc
11161 acattctgcg gcctctgtgc tctgcaagcc gcaaactttc accaatggac cagaactacc
11221 tgtgaaatta ataacagaca tactccaagc tgcctttgtg tgcttaatca cgtatactca
11281 cgtgctcaat agtcaccaat gccctccctc ttggccctct ccttttcttt tttcgaccga
11341 attgagctcg gtaccaaacg ttgctagcag ttccaggtgg aatgttatga tgagcattgt
11401 attaaatcag gagatatagc atgatctcta gttagctcac cacaaaagtc agacggcgta
11461 accaaaagtc acacaacaca agctgtaagg atttcggcac ggctacggaa gacggagaag
11521 ccaccttcag tggactcgag taccatttaa ttctatttgt gtttgatcga gacctaatac
11581 agcccctaca acgaccatca aagtcgtata gctaccagtg aggaagtgga ctcaaatcga
11641 cttcagcaac atctcctgga taaactttaa gcctaaacta tacagaataa gataggtgga
11701 gagcttatac cgagctccca aatctgtcca gatcatggtt gaccggtgcc tggatcttcc
11761 tatagaatca tccttattcg ttgacctagc tgattctgga gtgacccaga gggtcatgac
11821 ttgagcctaa aatccgccgc ctccaccatt tgtagaaaaa tgtgacgaac tcgtgagctc
11881 tgtacagtga ccggtgactc tttctggcat gcggagagac ggacggacgc agagagaagg
11941 gctgagtaat aagccactgg ccagacagct ctggcggctc tgaggtgcag tggatgatta
12001 ttaatccggg accggccgcc cctccgcccc gaagtggaaa ggctggtgtg cccctcgttg
12061 accaagaatc tattgcatca tcggagaata tggagcttca tcgaatcacc ggcagtaagc
12121 gaaggagaat gtgaagccag gggtgtatag ccgtcggcga aatagcatgc cattaaccta
12181 ggtacagaag tccaattgct tccgatctgg taaaagattc acgagatagt accttctccg
12241 aagtaggtag agcgagtacc cggcgcgtaa gctccctaat tggcccatcc ggcatctgta
12301 gggcgtccaa atatcgtgcc tctcctgctt tgcccggtgt atgaaaccgg aaaggccgct
12361 caggagctgg ccagcggcgc agaccgggaa cacaagctgg cagtcgaccc atccggtgct
12421 ctgcactcga cctgctgagg tccctcagtc cctggtaggc agctttgccc cgtctgtccg
12481 cccggtgtgt cggcggggtt gacaaggtcg ttgcgtcagt ccaacatttg ttgccatatt
12541 ttcctgctct ccccaccagc tgctcttttc ttttctcttt cttttcccat cttcagtata
12601 ttcatcttcc catccaagaa cctttatttc ccctaagtaa gtactttgct acatccatac
12661 tccatccttc ccatccctta ttcctttgaa cctttcagtt cgagctttcc cacttcatcg
12721 cagcttgact aacagctacc ccgcttgagc agacatcacc atggt
//