LOCUS Exported 7102 bp ds-DNA circular SYN 09-七月-2017
DEFINITION .
ACCESSION .
VERSION .
KEYWORDS pSIH1-ControlshRNA
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7102)
AUTHORS MiaoLingBio
TITLE Direct Submission
JOURNAL Exported 2017年7月9日 from MiaoLingPlasmid
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..7102
/organism="synthetic DNA construct"
/mol_type="other DNA"
LTR 234..414
/note="5' LTR (truncated)"
/note="truncated 5' long terminal repeat (LTR) from HIV-1"
misc_feature 458..583
/note="HIV-1 Psi"
/note="packaging signal of human immunodeficiency virus
type 1"
misc_feature 1076..1309
/note="RRE"
/note="The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to the
cytoplasm."
misc_feature 1798..1915
/note="cPPT/CTS"
/note="central polypurine tract and central termination
sequence of HIV-1"
promoter 2005..2208
/note="CMV promoter"
/note="human cytomegalovirus (CMV) immediate early
promoter"
CDS 2279..2878
/codon_start=1
/gene="pac from Streptomyces alboniger"
/product="puromycin N-acetyltransferase"
/note="Puro"
/note="confers resistance to puromycin"
/translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
misc_feature 2885..3473
/note="WPRE"
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
misc_feature 3726..3816
/note="H1 promoter"
misc_feature 3825..3881
/note="ControlshRNA"
LTR 3912..4092
/note="5' LTR (truncated)"
/note="truncated 5' long terminal repeat (LTR) from HIV-1"
polyA_signal 4164..4285
/note="SV40 poly(A) signal"
/note="SV40 polyadenylation signal"
rep_origin 4304..4439
/note="SV40 ori"
/note="SV40 origin of replication"
rep_origin complement(4879..5467)
/direction=LEFT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(5638..6498)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/note="Amp"
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(6499..6603)
/gene="bla"
/note="Amp promoter"
ORIGIN
1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacaata aacgggtctc
241 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
301 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
361 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
421 cccgaacagg gacctgaaag cgaaagggaa accagagctc tctcgacgca ggactcggct
481 tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt
541 gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag
601 aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt
661 aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt
721 agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg
781 atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag
841 gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag
901 taagaccacc gcacagcaag cggccgctga tcttcagacc tggaggagga gatatgaggg
961 acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag
1021 cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag
1081 ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc
1141 tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga
1201 gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc
1261 aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg
1321 gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata
1381 aatctctgga acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa
1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggttaacttt
1801 taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag acataatagc
1861 aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa attttatcga
1921 tactagtatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac
1981 gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga
2041 tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg
2101 ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg
2161 caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctcg tttagtgaac
2221 cgtcagatcg cctggagacg ccatccacgc tgttttgacc tccatagaag attctagaat
2281 gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac gacgtcccca gggccgtacg
2341 caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg atccggaccg
2401 ccacatcgag cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg ggctcgacat
2461 cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca cgccggagag
2521 cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc atggccgagt tgagcggttc
2581 ccggctggcc gcgcagcaac agatggaagg cctcctggcg ccgcaccggc ccaaggagcc
2641 cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac cagggcaagg gtctgggcag
2701 cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga
2761 gacctccgcg ccccgcaacc tccccttcta cgagcggctc ggcttcaccg tcaccgccga
2821 cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg gtgcctgagt
2881 cgacaatcaa cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt
2941 tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc
3001 ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga
3061 gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc
3121 cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct
3181 ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg
3241 gctgttgggc actgacaatt ccgtggtgtt gtcggggaag ctgacgtcct ttccatggct
3301 gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc
3361 cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgca
3421 tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcctggaat
3481 taattcgagc tcggtacctt taagaccaat gacttacaag gcagctgtag atcttagcca
3541 ctttttaaaa gaaaaggggg gactggaagg gctaattcac tcccaacgat gtcaagaatt
3601 ggaacgctga cgtcatcaac ccgctccaag gaatcgcggg cccagtgtca ctaggcggga
3661 acacccagcg cgcgtgcgcc ctggcaggaa gatggctgtg agggacaggg gagtggcgcc
3721 ctgcaatatt tgcatgtcgc tatgtgttct gggaaatcac cataaacgtg aaatgtcttt
3781 ggatttggga atcttataag ttctgtatga gaccacttgg atccaatagc gactaaacac
3841 atcaattcaa gagattgatg tgtttagtcg ctattttttt tgaattcttc gatctgcttt
3901 ttgcttctac tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac
3961 tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg
4021 cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga
4081 aaatctctag cagtagtagt tcatgtcatc ttattattca gtatttataa cttgcaaaga
4141 aatgaatatc agagagtgag aggaacttgt ttattgcagc ttataatggt tacaaataaa
4201 gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt
4261 tgtccaaact catcaatgta tcttatcatg tctggctcta gctatcccgc ccctaactcc
4321 gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc
4381 cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct
4441 agacttttgc agagacggcc caaattcgta atcatggtca tagctgtttc ctgtgtgaaa
4501 ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg
4561 gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca
4621 gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg
4681 tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg
4741 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
4801 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
4861 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
4921 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
4981 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
5041 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
5101 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
5161 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
5221 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
5281 gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc
5341 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
5401 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
5461 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
5521 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
5581 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta
5641 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
5701 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
5761 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca
5821 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
5881 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt
5941 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag
6001 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt
6061 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat
6121 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt
6181 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc
6241 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat
6301 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag
6361 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
6421 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
6481 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta
6541 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc
6601 gcgcacattt ccccgaaaag tgccacctga cgtctaagaa accattatta tcatgacatt
6661 aacctataaa aataggcgta tcacgaggcc ctttcgtctc gcgcgtttcg gtgatgacgg
6721 tgaaaacctc tgacacatgc agctcccgga gacggtcaca gcttgtctgt aagcggatgc
6781 cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc ggggctggct
6841 taactatgcg gcatcagagc agattgtact gagagtgcac catatgcggt gtgaaatacc
6901 gcacagatgc gtaaggagaa aataccgcat caggcgccat tcgccattca ggctgcgcaa
6961 ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta cgccagctgg cgaaaggggg
7021 atgtgctgca aggcgattaa gttgggtaac gccagggttt tcccagtcac gacgttgtaa
7081 aacgacggcc agtgccaagc tg
//