Recombinant Human sRANK Receptor/TNFRSF11A
产品编号
产品名称
产品包装
产品价格
XY417-1mg
1mg
5665.00元
Species
Gene ID
Accession
Source
Length
MW
Tag
Human
8792
Q9Y6Q6
E. coli
174aa
19.1kDa
－
About this Protein
Name
Recombinant Human sRANK Receptor/TNFRSF11A (Recombinant Human soluble Receptor Activator of Nuclear Factor kappa-B Receptor; rHusRANK Receptor)；重组人可溶性核因子κB受体激活剂/TNFRSF11A
Synonyms
CD265; ODFR; TNFRSF11A; TRANCE R; CD265; CD265 antigen; FEO; loss of heterozygosity, 18, chromosomal region 1; ODFROSTS; OFE; OPTB7; Osteoclast differentiation factor receptor; PDB2; RANKLOH18CR1; Receptor activator of NF-KB; receptor activator of nuclear factor-kappa B; TRANCER; tumor necrosis factor receptor superfamily member 11A; tumor necrosis factor receptor superfamily, member 11a, activator of NFKB; tumor necrosis factor receptor superfamily, member 11a, NFKB activator
Purity
>98% by SDS-PAGE and HPLC analyses.
Biological Activity
Fully biologically active when compared to standard. The ED50 as determined by its ability to inhibit sRANK Ligand induced nuclear factor kappa B(NFkappaB) in RAW 264.7 cells is less than 50ng/ml, corresponding to a specific activity of >2.0×104IU/mg in the presence of 15ng/ml of recombinant sRANK Ligand.
Physical Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation
Lyophilized from a 0.2μm filtered concentrated solution in 20mM Tris-HCl, pH8.0, 150mM NaCl.
Endotoxin
Less than 0.1EU/μg of rHusRANK Receptor as determined by LAL method.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in appropriate buffered solutions.
Category
Cytokine
Background
RANK Ligand and RANK Receptor are members of the TNF superfamily that play an important role in the regulation of specific immunity and bone turnover. RANK Receptor was originally identified as a dendritic-cell-membrane protein, which by interacting with RANKL augments the ability of dendritic cells to stimulate naïve T cell proliferation and to promote the survival of RANK + T cells. RANK is also expressed in a variety of tissues including skeletal muscle, thymus, liver, colon, small intestine and adrenal gland. The RANK/RANKL interaction is important in the regulation of osteoclastogenesis and in dendritic-cellmediated T cell immune responses. Impairments in RANK signaling have been implicated in the induction of expansile osteolysis and Paget disease of bone (PDB2). Recombinant human sRANK receptor is a 19.1kDa polypeptide containing the TNFR homologous cysteine rich portion of the extracellular domain of RANK receptor (174 amino acid residues).
Amino Acid Sequence
QIAPPCTSEK HYEHLGRCCN KCEPGKYMSS KCTTTSDSVC LPCGPDEYLD SWNEEDKCLL HKVCDTGKAL VAVVAGNSTT PRRCACTAGY HWSQDCECCR RNTECAPGLG AQHPLQLNKD TVCKPCLAGY FSDAFSSTDK CRPWTNCTFL GKRVEHHGTE KSDAVCSSSL PARK
包装清单:
包装
XY417-20μg
20μg
XY417-100μg
100μg
-
说明书
1份
保存条件: -20℃或更低温度保存，至少一年有效。由于蛋白的每次冻融均会引起部分失活，所以**配制成相应浓度的储存液后(请根据产品简介中Reconstitution一栏的信息配制储存液)，须分装后-20℃或更低温度冻存，以避免反复冻融。 注意事项: 由于有些塑料管壁对某些蛋白有较强的吸附作用，溶液中的蛋白很容易粘附在管壁上，并且粘附后的蛋白很难与管壁分离。而载体蛋白(Carrier protein，如0.1% BSA等)的主要作用是预先封闭塑料管壁上的蛋白结合位点，使细胞因子或重组蛋白不会粘附于管壁。所以一定要使用产品简介中Reconstitution一栏的信息配制储存液。 本产品**于专业人员的科学研究用，不得用于临床诊断或**，不得用于食品或药品，不得存放于普通住宅内。 为了您的**和健康，请穿实验服并戴一次性手套操作。