Recombinant Human Noggin
产品编号
产品名称
产品包装
产品价格
XY5786-1mg
1mg
20076.00元
Species
Gene ID
Accession
Source
Length
MW
Tag
Human
9241
Q13253
E. coli
206aa
46.3kDa
－
About this Antibody
Name
Recombinant Human Noggin (Recombinant Human Noggin; rHuNoggin)；重组人头蛋白
Synonyms
NOG; SYM1; SYNS1; noggin; SYM1; symphalangism 1 (proximal); synostoses (multiple) syndrome 1; SYNS1
Purity
>95% by SDS-PAGE and HPLC analyses.
Biological Activity
Fully biologically active when compared to standard. The ED50 as determined by inhibiting BMP-4-induced alkaline phosphatase production of murine ATDC5 cells is less than 3.0ng/ml, corresponding to a specific activity of >3.3×105IU/mg in the presence of 5ng/ml rHuBMP-4.
Physical Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation
Lyophilized from a 0.2μM filtered concentrated solution in 30% acetonitrile, 0.1% TFA.
Endotoxin
Less than 1EU/μg of rHuNoggin as determined by LAL method.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in 10mM HAc to a concentration of 0.1-1.0mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in appropriate buffered solutions.
Category
Cytokine
Background
Noggin encoded by the NOG gene, was first isolated from Xenopus, having the function of inducing secondary axis formation in frog embryos. It inhibits TGF-β family ligands and preventing them from binding to their corresponding receptors. Noggin was originally found as a BMP-4 antagonist, and then has been shown to modulate the activities of other BMPs (BMP-2, 7, 13 and 14). Additionally, it has pleiotropic effect, both in early development and later stages. The results of the mouse knockout of noggin suggest that it is involved in numerous developmental processes, such as neural tube fusion and joint formation. In recent report, proximal symphalangism (SYM1) and multiple synostoses syndrome (SYNS1) have relation with the mutant of evolutionarily conserved amino acid residues of Noggin. Mature human Noggin shares 99%, 99%, 98%, 97% and 89%a.a. sequence identity with mouse, rat, bovine, equine and chicken Noggin, respectively.
Amino Acid Sequence
MQHYLHIRPA PSDNLPLVDL IEHPDPIFDP KEKDLNETLL RSLLGGHYDP GFMATSPPED RPGGGGGAAG GAEDLAELDQ LLRQRPSGAM PSEIKGLEFS EGLAQGKKQR LSKKLRRKLQ MWLWSQTFCP VLYAWNDLGS RFWPRYVKVG SCFSKRSCSV PEGMVCKPSK SVHLTVLRWR CQRRGGQRCG WIPIQYPIIS ECKCSC
包装清单:
包装
XY5786-5μg
5μg
XY5786-20μg
20μg
XY5786-100μg
100μg
-
说明书
1份
保存条件: -20℃或更低温度保存，至少一年有效。由于蛋白的每次冻融均会引起部分失活，所以**配制成相应浓度的储存液后(请根据产品简介中Reconstitution一栏的信息配制储存液)，须分装后-20℃或更低温度冻存，以避免反复冻融。 注意事项: 由于有些塑料管壁对某些蛋白有较强的吸附作用，溶液中的蛋白很容易粘附在管壁上，并且粘附后的蛋白很难与管壁分离。而载体蛋白(Carrier protein，如0.1% BSA等)的主要作用是预先封闭塑料管壁上的蛋白结合位点，使细胞因子或重组蛋白不会粘附于管壁。所以一定要使用产品简介中Reconstitution一栏的信息配制储存液。 本产品**于专业人员的科学研究用，不得用于临床诊断或**，不得用于食品或药品，不得存放于普通住宅内。 为了您的**和健康，请穿实验服并戴一次性手套操作。