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Recombinant Murine TNF-α/TNFSF2

Recombinant Murine TNF-α/TNFSF2
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  • 产品名称:Recombinant Murine TNF-α/TNFSF2
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  • 产品展商:XYbscience
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简单介绍
Recombinant Murine TNF-α/TNFSF2由于有些塑料管壁对某些蛋白有较强的吸附作用,溶液中的蛋白很容易粘附在管壁上,并且粘附后的蛋白很难与管壁分离。而载体蛋白(Carrier protein,如0.1% BSA等)的主要作用是预先封闭塑料管壁上的蛋白结合位点,使细��因子或重组蛋白不会粘附于管壁。Recombinant Murine TNF-α/TNFSF2所以一定要使用产品简介中Reconstitution一栏的信息配制储存液。
产品描述

Recombinant Murine TNF-α/TNFSF2

产品编号

产品名称

产品包装

产品价格

XY6020-1mg

Recombinant Murine TNF-α/TNFSF2

1mg

15444.00


Species

Gene ID

Accession

Source

Length

MW

Tag

Mouse

21926

P06804

E. coli

157aa

17.4kDa


About this Antibody

Name

Recombinant Murine TNF-α/TNFSF2 (Recombinant Murine Tumor Necrosis Factor-α; rMuTNF-α)Recombinant Mouse TNF-α/TNFSF2 ; 重组小鼠肿瘤坏死因子α

Synonyms

Cachetin; DIF; TNF; tnfa; tnf-a; TNFSF1A; TNFSF2; APC1 protein; Cachectin; DIF; TNF, monocyte-derived; TNF-a; TNF-alphacachectin; TNFATNF, macrophage-derived; TNFSF2TNF superfamily, member 2; tumor necrosis factor; tumor necrosis factor (TNF superfamily, member 2); tumor necrosis factor alpha; Tumor necrosis factor ligand superfamily member 2; tumor necrosis factor-alpha

Purity

>98% by SDS-PAGE and HPLC analyses.

Biological Activity

Fully biologically active when compared to standard. The ED50 as determined by a cytotoxicity assay using murine L929 cells is less than 0.1ng/ml, corresponding to a specific activity of >1.0×107IU/mg in the presence of actinomycin D.

Physical Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation

Lyophilized from a 0.2μm filtered solution in PBS, pH7.2.

Endotoxin

Less than 1EU/μg of rMuTNF-α as determined by LAL method.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/ml. Stock solutions should be apportioned into working aliquots and stored at -20. Further dilutions should be made in appropriate buffered solutions.

Category

Cytokine

Background

Tumor necrosis factor alpha (TNF-α), also called cachectin, is the best-know member of the TNF-family, which can cause cell death. This protein is produced by neutrophils, activated lymphocytes, macrophages, NK cells, LAK cells, astrocytes endothelial cells, smooth muscle cells and some transformed cells. TNF-α occurs as a secreted, soluble form and as a membrane-anchored form, both of which are biologically active. The naturally-occurring form of TNF-α is glycosylated, but non-glycosylated recombinant TNF-α has comparable biological activity. The biologically active native form of TNF-α is reportedly a trimer. Human and murine TNF-α show approximately 79% homology at the amino acid level and cross-reactivity between the two species. Two types of receptors for TNF-α have been described and virtually all cell types studied show the presence of one or both of these receptor types.

Amino Acid Sequence

MLRSSSQNSS DKPVAHVVAN HQVEEQLEWL SQRANALLAN GMDLKDNQLV VPADGLYLVY SQVLFKGQGC PDYVLLTHTV SRFAISYQEK VNLLSAVKSP CPKDTPEGAE LKPWYEPIYL GGVFQLEKGD QLSAEVNLPK YLDFAESGQV YFGVIAL

包装清单:

产品编号

产品名称

包装

XY6020-5μg

Recombinant Murine TNF-α/TNFSF2

5μg

XY6020-20μg

Recombinant Murine TNF-α/TNFSF2

20μg

XY6020-100μg

Recombinant Murine TNF-α/TNFSF2

100μg

XY6020-1mg

Recombinant Murine TNF-α/TNFSF2

1mg

-

说明书

1

保存条件:
       -20℃或更低温度保存,至少一年有效。由于蛋白的每次冻融均会引起部分失活,所以**配制成相应浓度的储存液后(请根据产品简介中Reconstitution一栏的信息配制储存液),须分装后-20℃或更低温度冻存,以避免反复冻融。
注意事项:
       由于有些塑料管壁对某些蛋白有较强的吸附作用,溶液中的蛋白很容易粘附在管壁上,并且粘附后的蛋白很难与管壁分离。而载体蛋白(Carrier protein,如0.1% BSA)的主要作用是预先封闭塑料管壁上的蛋白结合位点,使细胞因子或重组蛋白不会粘附于管壁。所以一定要使用产品简介中Reconstitution一栏的信息配制储存液。
       本产品**于专业人员的科学研究用,不得用于临床诊断或**,不得用于食品或药品,不得存放于普通住宅内。
       为了您的**和健康,请穿实验服并戴一次性手套操作。

 

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