Recombinant Human TIMP-2
产品编号
产品名称
产品包装
产品价格
XY7041-1mg
1mg
26773.00元
Species
Gene ID
Accession
Source
Length
MW
Tag
Human
7077
P16035
E. coli
194aa
21.8kDa
－
About this Antibody
Name
Recombinant Human TIMP-2 (Recombinant Human Tissue Inhibitor of Metalloproteinases 2; rHuTIMP-2)；重组人组织金属蛋白酶抑制剂2
Synonyms
CSC-21Ktissue inhibitor of metalloproteinase 2; metalloproteinase inhibitor 2; TIMP metallopeptidase inhibitor 2; TIMP-2; Tissue inhibitor of metalloproteinases 2
Purity
>95% by SDS-PAGE and HPLC analyses.
Biological Activity
Fully biologically active when compared to standard. The biologically active as determined by its ability to inhibit humanmMP-2 cleavage of a fluorogenic peptide substrate Mca-PLGL-Dpa-ARNH2.
Physical Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation
Lyophilized from a 0.2μm filtered concentrated solution in PBS, pH7.4, with 3% trehalose.
Endotoxin
Less than 1EU/μg of rHuTIMP-2 as determined by LAL method.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in appropriate buffered solutions.
Category
Enzymes
Background
TIMPs-1 through -4 regulate the activity of zinc metalloproteases known asmMPs, ADAMs and ADAMTSs. Structurally, TIMPs contain two domains. The N-terminal domain binds to the active site of mature metalloproteases via a 1:1 non-covalent interaction, blocking access of substrates to the catalytic site. In addition, The C-terminal domain of TIMP-1 and TIMP-2 binds to the hemopexin-like domain of pro-MMP-9 and pro-MMP-2, respectively. The latter binding is essential for the cell surface activation ofmMP-2 bymMP-14.
Amino Acid Sequence
CSCSPVHPQQ AFCNADVVIR AKAVSEKEVD SGNDIYGNPI KRIQYEIKQI KMFKGPEKDI EFIYTAPSSA VCGVSLDVGG KKEYLIAGKA EGDGKMHITL CDFIVPWDTL STTQKKSLNH RYQMGCECKI TRCPMIPCYI SSPDECLWMD WVTEKNINGH QAKFFACIKR SDGSCAWYRG AAPPKQEFLD IEDP
包装清单:
包装
XY7041-2μg
2μg
XY7041-10μg
10μg
XY7041-100μg
100μg
-
说明书
1份
保存条件: -20℃或更低温度保存，至少一年有效。由于蛋白的每次冻融均会引起部分失活，所以**配制成相应浓度的储存液后(请根据产品简介中Reconstitution一栏的信息配制储存液)，须分装后-20℃或更低温度冻存，以避免反复冻融。 注意事项: 由于有些塑料管壁对某些蛋白有较强的吸附作用，溶液中的蛋白很容易粘附在管壁上，并且粘附后的蛋白很难与管壁分离。而载体蛋白(Carrier protein，如0.1% BSA等)的主要作用是预先封闭塑料管壁上的蛋白结合位点，使细胞因子或重组蛋白不会粘附于管壁。所以一定要使用产品简介中Reconstitution一栏的信息配制储存液。 本产品**于专业人员的科学研究用，不得用于临床诊断或**，不得用于食品或药品，不得存放于普通住宅内。 为了您的**和健康，请穿实验服并戴一次性手套操作。