Recombinant Bovine EK, Yeast
产品编号
产品名称
产品包装
产品价格
XY7055-1000IU
1000IU
2662.00元
Species
Gene ID
Accession
Source
Length
MW
Tag
Bovine
282009
P98072
P. pastoris
235aa
43kDa
－
About this Protein
Name
Recombinant Bovine EK, Yeast (Recombinant Bovine Enterokinase Light Chain; rBoEK)；重组牛轻链肠激酶
Synonyms
Enterokinase; ENTK; PRSS7; TMPRSS15; EC 3.4.21; EC 3.4.21.9; Enterokinase; ENTKenterokinase; MGC133046; protease, serine, 7 (enterokinase); PRSS7enteropeptidase; Serine protease 7; Transmembrane protease serine 15; transmembrane protease, serine 15
Purity
N/A
Biological Activity
Physical Appearance
Sterile liquid.
Formulation
50mM Tris-HCl, pH8.0, 0.5M NaCl and 50% glycerol.
Endotoxin
Less than 1EU/μg of rBoEKL as determined by LAL method.
Reconstitution
Category
Enzymes
Background
Enterokinase (EK) is an amino protease existing in duodenum of mammal and is involved in digestion. It consists of a disulfidelinked 82–140kDa heavy chain which anchors enterokinase in the intestinal brush border membrane and a 35–62kDa light chain which contains the catalytic subunit. Additionally, both of the chains are derived from a single precursor that is cleaved by a trypsin-like protease. EK can specially recognize the amino acid sequence DDDDK, and digest the peptide bond after the lysine residue. rEK was report to be more effective than nature EK in cleaving recombinant proteins. Furthermore, the light chain possesses the whole enzyme activity of EK. rBoEK has the highest activity than EK of other species and is used wildly in biochemical applications.
Amino Acid Sequence
包装清单:
包装
XY7055-100IU
100IU
XY7055-250IU
250IU
-
说明书
1份
保存条件: -20℃或更低温度保存，至少一年有效。由于蛋白的每次冻融均会引起部分失活，所以**配制成相应浓度的储存液后(请根据产品简介中Reconstitution一栏的信息配制储存液)，须分装后-20℃或更低温度冻存，以避免反复冻融。 注意事项: 由于有些塑料管壁对某些蛋白有较强的吸附作用，溶液中的蛋白很容易粘附在管壁上，并且粘附后的蛋白很难与管壁分离。而载体蛋白(Carrier protein，如0.1% BSA等)的主要作用是预先封闭塑料管壁上的蛋白结合位点，使细胞因子或重组蛋白不会粘附于管壁。所以一定要使用产品简介中Reconstitution一栏的信息配制储存液。 本产品**于专业人员的科学研究用，不得用于临床诊断或**，不得用于食品或药品，不得存放于普通住宅内。 为了您的**和健康，请穿实验服并戴一次性手套操作。