Recombinant Tobacco EV
产品编号
产品名称
产品包装
产品价格
XY7068-10000IU
10000IU
8195.00元
Species
Gene ID
Accession
Source
Length
MW
Tag
Tobacco
1502321
P04517
E. coli
－
About this Protein
Name
Recombinant Tobacco EV (Recombinant Tobacco Etch Virus Protease; rTEV)；重组**蚀纹病毒蛋白酶
Synonyms
polyprotein; 6K2 protein; NIa－VPg protein; NIb protein; coat protein; P1 protein; HC－Pro protein; P3 protein; 6K1 protein; CI protein; NIa－Pro protein
Purity
>90% by SDS-PAGE analysis.
Biological Activity
N/A
Physical Appearance
Clear colorless liquid.
Formulation
A 0.2μm filtered solution in 25mM Tris－HCl, pH8.0, 75mM NaCl, 5mM EDTA, 10mM GSH, with 50% Glycerol.
Endotoxin
Reconstitution
A number of variables can be changed to optimize the cleavage of any specific protein. The amount of rTEV, the temperature of the incubation, and the time needed for cleavage may be examined. If the protein of interest is heat－labile, then 4℃ incubations are recommended. Reactions at 4℃ will require longerincubation times and/or more rTEV.
Category
Enzymes
Background
TEV protease encoded by the tobacco etch virus is a catalytic domain of the Nuclear Inclusion a (NIa) protein. It is consists of 241a.a. with the molecular weight of 27kDa. TEV recognizes the amino acid sequence of the general form E-X-X-Y-X-Q (or S)/X', and cleaves between Q (or S)/X'. In this form X and X' stand for any of the amino acid residues, except that X' cannot be P. The optimal cleavage site is ENLYFQ/G. As having the absolute specificity and wildly using conditions like broad pH range and ionic strength, the TEV protease became more versatile than EK, thrombin and other protease used in biochemical applications, especially recombinant protein production. The optimal temperature for cleavage is 30℃; however, the enzyme can be used at temperatures as low as 4℃. Following digestion, TEV Protease can be removed from the reaction via the GST tag sequence by affinity chromatography.
Amino Acid Sequence
包装清单:
包装
XY7068-300IU
300IU
XY7068-1000IU
1000IU
-
说明书
1份
保存条件: -20℃或更低温度保存，至少一年有效。由于蛋白的每次冻融均会引起部分失活，所以**配制成相应浓度的储存液后(请根据产品简介中Reconstitution一栏的信息配制储存液)，须分装后-20℃或更低温度冻存，以避免反复冻融。 注意事项: 由于有些塑料管壁对某些蛋白有较强的吸附作用，溶液中的蛋白很容易粘附在管壁上，并且粘附后的蛋白很难与管壁分离。而载体蛋白(Carrier protein，如0.1% BSA等)的主要作用是预先封闭塑料管壁上的蛋白结合位点，使细胞因子或重组蛋白不会粘附于管壁。所以一定要使用产品简介中Reconstitution一栏的信息配制储存液。 本产品**于专业人员的科学研究用，不得用于临床诊断或**，不得用于食品或药品，不得存放于普通住宅内。 为了您的**和健康，请穿实验服并戴一次性手套操作。