Recombinant Human UBE2L3, His-tag
产品编号
产品名称
产品包装
产品价格
XY7109-1mg
1mg
12345.00元
Species
Gene ID
Accession
Source
Length
MW
Tag
Human
7332
P68036
E. coli
162aa
18.9kDa
His
About this Antibody
Name
Recombinant Human UBE2L3, His-tag (Recombinant Human Ubiquitin Conjugating Enzyme E2 L3, His-tag; rHuUBE2L3, His-tag)；重组人泛素结合酶E2L3，His标签
Synonyms
E2-F1; L-UBC; UBCE7; UbcM4; UBE2L3; E2-F1; EC 6.3.2.19; L-UBC; UBCE7; UbcH7; UBCH7UbcM4; Ubiquitin carrier protein L3; ubiquitin-conjugating enzyme E2 L3; Ubiquitin-conjugating enzyme E2-F1; ubiquitin-conjugating enzyme E2L 3; ubiquitin-conjugating enzyme UBCH7; Ubiquitin-protein ligase L3
Purity
>95% by SDS-PAGE and HPLC analyses.
Biological Activity
Data is not available.
Physical Appearance
Sterile Colorless liquid.
Formulation
A 0.2μm filtered concentrated solution in 50mM HEPES, pH7.0, with 125mM NaCl, 10% Glycerol, 5% Trehalose, 1mM DTT.
Endotoxin
Less than 1EU/μg of rHuUBE2L3, His-tag as determined by LAL method.
Reconstitution
N/A
Category
Ubiquitins
Background
Ubiquitin-conjugating enzyme E2 L3 belongs to the ubiquitin-conjugating enzyme family and is encoded by the UBE2L3 gene in humans. The ubiquitin-conjugating enzymes, also known as E2 enzymes and more rarely as ubiquitin-carrier enzymes, take part in the second step in the ubiquitination reaction. In this reaction, E1 activates the ubiquitin by covalently attaching the molecule to its active site cysteine residue. The activated ubiquitin is then transferred to an E2 cysteine and then the E2 molecule binds E3 via a structurally conserved binding region. The UBE2L3 specifically acts with HECT-type and RBR family E3 ubiquitin-protein shown to interact with UBOX5, ARIH1, Cbl gene, UBE3A and NEDD4. Down-regulated during the S-phase it is involved in progression through the cell cycle. Additionally, UBE2L3 regulates nuclear hormone receptors transcriptional activity and plays a role in myelopoiesis.
Amino Acid Sequence
MHHHHHHAMA ASRRLMKELE EIRKCGMKNF RNIQVDEANL LTWQGLIVPD NPPYDKGAFR IEINFPAEYP FKPPKITFKT KIYHPNIDEK GQVCLPVISA ENWKPATKTD QVIQSLIALV NDPQPEHPLR ADLAEEYSKD RKKFCKNAEE FTKKYGEKRP VD
包装清单:
包装
XY7109-10μg
10μg
XY7109-50μg
50μg
XY7109-100μg
100μg
-
说明书
1份
保存条件: -20℃或更低温度保存，至少一年有效。由于蛋白的每次冻融均会引起部分失活，所以**配制成相应浓度的储存液后(请根据产品简介中Reconstitution一栏的信息配制储存液)，须分装后-20℃或更低温度冻存，以避免反复冻融。 注意事项: 由于有些塑料管壁对某些蛋白有较强的吸附作用，溶液中的蛋白很容易粘附在管壁上，并且粘附后的蛋白很难与管壁分离。而载体蛋白(Carrier protein，如0.1% BSA等)的主要作用是预先封闭塑料管壁上的蛋白结合位点，使细胞因子或重组蛋白不会粘附于管壁。所以一定要使用产品简介中Reconstitution一栏的信息配制储存液。 本产品**于专业人员的科学研究用，不得用于临床诊断或**，不得用于食品或药品，不得存放于普通住宅内。 为了您的**和健康，请穿实验服并戴一次性手套操作。