Recombinant Human UBE2I, His-tag
产品编号
产品名称
产品包装
产品价格
XY7113-1mg
1mg
12354.00元
Species
Gene ID
Accession
Source
Length
MW
Tag
Human
7329
P63279
E. coli
158aa
19.5kDa
His
About this Antibody
Name
Recombinant Human UBE2I, His-tag (Recombinant Human SUMO-Conjugating Enzyme UBC9, His-tag; rHuUBE2I, His-tag)；重组人泛素结合酶E2I，His标签
Synonyms
p18; SUMO-protein Ligase; Ubc9; UBCE9; C358B7.1; EC 6.3.2; EC 6.3.2.-; P18; p18; SUMO-1-protein ligase; SUMO-protein ligase; UBC9SUMO-conjugating enzyme UBC9; UBCE9; Ubiquitin carrier protein 9; Ubiquitin carrier protein I; ubiquitin conjugating enzyme 9; Ubiquitin-conjugating enzyme E2 I; ubiquitin-conjugating enzyme E2I (homologous to yeast UBC9); ubiquitin-conjugating enzyme E2I (UBC9 homolog, yeast); ubiquitin-conjugating enzyme UbcE2A; ubiquitin-like protein SUMO-1 conjugating enzyme; ubiquitin-protein ligase E2I; Ubiquitin-protein ligase I
Purity
>95% by SDS-PAGE and HPLC analyses.
Biological Activity
Data is not available.
Physical Appearance
Sterile Colorless liquid.
Formulation
A 0.2μm filtered concentrated solution in 50mM HEPES, pH7.6, with 125mM NaCl, 10% Glycerol, 1mM DTT.
Endotoxin
Less than 1EU/μg of rHuUBC9, His-tag as determined by LAL method.
Reconstitution
N/A
Category
Ubiquitins
Background
SUMO-Conjugating Enzyme UBC9 belongs to the ubiquitin-conjugating enzyme family and is encoded by the UBE2I gene in humans. The ubiquitin-conjugating enzymes, also known as E2 enzymes and more rarely as ubiquitin-carrier enzymes, take part in the second step in the ubiquitination reaction. In this reaction, E1 activates the ubiquitin by covalently attaching the molecule to its active site cysteine residue. The activated ubiquitin is then transferred to an E2 cysteine and then the E2 molecule binds E3 via a structurally conserved binding region. The UBC9 accepts the ubiquitin-like proteins SUMO1-4 from the UBLE1AUBLE1B E1 complex and catalyzes their covalent attachment to other proteins with the help of an E3 ligase such as RANBP2 or CBX4. Additionally, It takes parts in catalysis the formation of poly-SUMO chains, sumoylation of FOXL2 and KAT5, and the segregation of nuclear architecture and chromosome.
Amino Acid Sequence
MHHHHHHAMG TLNMSGIALS RLAQERKAWR KDHPFGFVAV PTKNPDGTMN LMNWECAIPG KKGTPWEGGL FKLRMLFKDD YPSSPPKCKF EPPLFHPNVY PSGTVCLSIL EEDKDWRPAI TIKQILLGIQ ELLNEPNIQD PAQAEAYTIY CQNRVEYEKR VRAQAKKFAP S
包装清单:
包装
XY7113-10μg
10μg
XY7113-50μg
50μg
XY7113-100μg
100μg
-
说明书
1份
保存条件: -20℃或更低温度保存，至少一年有效。由于蛋白的每次冻融均会引起部分失活，所以**配制成相应浓度的储存液后(请根据产品简介中Reconstitution一栏的信息配制储存液)，须分装后-20℃或更低温度冻存，以避免反复冻融。 注意事项: 由于有些塑料管壁对某些蛋白有较强的吸附作用，溶液中的蛋白很容易粘附在管壁上，并且粘附后的蛋白很难与管壁分离。而载体蛋白(Carrier protein，如0.1% BSA等)的主要作用是预先封闭塑料管壁上的蛋白结合位点，使细胞因子或重组蛋白不会粘附于管壁。所以一定要使用产品简介中Reconstitution一栏的信息配制储存液。 本产品**于专业人员的科学研究用，不得用于临床诊断或**，不得用于食品或药品，不得存放于普通住宅内。 为了您的**和健康，请穿实验服并戴一次性手套操作。