Recombinant Human MIA
产品编号
产品名称
产品包装
产品价格
XY7266-1mg
1mg
20076.00元
Species
Gene ID
Accession
Source
Length
MW
Tag
Human
8190
Q16674
E. coli
107aa
12.1kDa
－
About this Antibody
Name
Recombinant Human MIA (Recombinant Human Melanoma Inhibitor Activity Protein; rHuMIA)；重组人黑色素瘤活性抑制蛋白
Synonyms
CD-RAP; CD-RAP; melanoma inhibitory activity; Melanoma inhibitory activity protein; melanoma-derived growth regulatory protein
Purity
>98% by SDS-PAGE and HPLC analyses.
Biological Activity
Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using human A375 cell line is less than 5μg/ml, corresponding to a specific activity of >200IU/mg.
Physical Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation
Lyophilized from a 0.2μm filtered concentrated solution in PBS, pH7.4, with 5% Trehalose.
Endotoxin
Less than 0.1EU/μg of rHuMIA as determined by LAL method.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in appropriate buffered solutions.
Category
Others
Background
MIA is an autocrine growth regulatory protein, secreted from chondrocytes and malignant melanoma cells, that promotes melanoma metastasis by binding competitively to fibronectin and laminin in a manner that results in melanoma cell detachment from the extracellular matrix in vivo. Elevated levels of MIA may represent a clinically useful marker for diagnosis of melanoma metastasis, as well as a potential marker for rheumatoid arthritis. The MIA is a member of the MIA/OTOR family, which also includes MIA, OTOR, and TANGO, and they share a Src homology-3 (SH3)-like domain. Recombinant Human MIA is a 12.1kDa globular protein containing 107 amino acid residues, including two intramolecular disulfide bonds.
Amino Acid Sequence
GPMPKLADRK LCADQECSHP ISMAVALQDY MAPDCRFLTI HRGQVVYVFS KLKGRGRLFW GGSVQGDYYG DLAARLGYFP SSIVREDQTL KPGKVDVKTD KWDFYCQ
包装清单:
包装
XY7266-5μg
5μg
XY7266-20μg
20μg
XY7266-100μg
100μg
-
说明书
1份
保存条件: -20℃或更低温度保存，至少一年有效。由于蛋白的每次冻融均会引起部分失活，所以**配制成相应浓度的储存液后(请根据产品简介中Reconstitution一栏的信息配制储存液)，须分装后-20℃或更低温度冻存，以避免反复冻融。 注意事项: 由于有些塑料管壁对某些蛋白有较强的吸附作用，溶液中的蛋白很容易粘附在管壁上，并且粘附后的蛋白很难与管壁分离。而载体蛋白(Carrier protein，如0.1% BSA等)的主要作用是预先封闭塑料管壁上的蛋白结合位点，使细胞因子或重组蛋白不会粘附于管壁。所以一定要使用产品简介中Reconstitution一栏的信息配制储存液。 本产品**于专业人员的科学研究用，不得用于临床诊断或**，不得用于食品或药品，不得存放于普通住宅内。 为了您的**和健康，请穿实验服并戴一次性手套操作。