Choline Kinase (Crude Enzyme)

Choline Kinase (Crude Enzyme)

       Choline Kinase，中文名为胆碱激酶，以三磷酸腺苷(ATP)作为磷酸的供体，催化胆碱发生磷酸化。本产品为大肠杆菌表达的胆碱激酶，并经简单纯化获得的可以确保达到一定酶活力的粗酶液。
       由于本产品需新鲜制备，确认订购后约6个工作日才可以发货。
       本产品可以提供十公斤、百公斤乃至吨级的粗酶液。
       Choline kinase (also known as CK,ChoK and choline phosphokinase) is an enzyme which catalyzes the first
reaction in the choline pathway for phosphatidylcholine (PC) biosynthesis. This reaction involves the transfer of a phosphate group from adenosine triphosphate (ATP) to choline in order to form phosphocholine. Thus, the two substrates of this enzyme are ATP and choline, whereas its two products are adenosine diphosphate (ADP) and O-phosphocholine. Choline kinase requires magnesium ions (+2) as a cofactor for this reaction. This enzyme belongs to the family of transferases, specifically those transferring phosphorus-containing groups (phosphotransferases) with an alcohol group as acceptor. The first detailed investigation of the enzyme was conducted by McCamen in 1962, where it was shown that the brain is the richest source of the enzyme in mammalian tissue. A related enzyme, ethanolamine kinase, tends to co-purify with choline kinase leading to a suggestion that the two activities are mediated by two distinct active sites on a single protein. These enzymes participate in glycine, serine and threonine metabolism and glycerophospholipid metabolism. In mammalian cells, the enzyme exists as three isoforms: CKα-1,CKα-2 and CKβ. These isoforms are encoded by two separate genes, CHKA and CHKB and are only active in their homodimeric, heterodimeric and oligomeric forms. This product with the indicated enzyme activity was briefly purified from engineered E.coli.
产品详细信息见下表:

包装清单：

保存条件：
       -20℃或更低温度保存，至少1个月有效。
注意事项：
       由于本产品需新鲜制备，在您确认订购后约6个工作日才能发货。
       粗酶液的每次冻融均可能会引起部分失活，所以收到酶液后请根据需要进行分装，并置于-20℃或更低温度保存。
       随着保存时间的延长，酶活力会有一定程度的下降，收到产品后应尽快使用。
       本产品在生产和保存的过程中可能会有混浊或沉淀产生，融化后混匀即可，不影响正常使用。
       本产品**于专业人员的科学研究用，不得用于临床诊断或**，不得用于食品或药品，不得存放于普通住宅内。
       为了您的**和健康，请穿实验服并戴一次性手套操作。

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