N-Cadherin Rabbit Polyclonal Antibody
产品编号
产品名称
产品包装
产品价格
XY0243
50μl
416.00元
来源
用途
交叉反应性
分子量
Rabbit
WB, FC, IHC, ICC, IF
H, M
140KDa
WB, Western blot; IP, Immunoprecipitation; IF, Immunofluorescence; IHC, Immunohistochemistry; ICC, Immunocytochemistry; FC, Flow Cytometry; ELISA, Enzyme-linked Immunosorbent Assay; ChIP, Chromatin Immunoprecipitation Assay. H, Human; M, Mouse; R, Rat; C, Chicken; Cw, Cow; Dg, Dog; Gp, Guinea pig; Hm, Hamster; Hr, Horse; Mk, Monkey; Pg, Pig; Rb, Rabbit; S, Sheep; Z, ZebrXYish; All, all species expected. 配套提供了Western一抗稀释液,可以用于Western检测或其它适当用途时的一抗稀释。 建议抗体使用时的稀释比例如下(实际使用时需根据抗原水平的高低作适当调整):
WB
IP
IF
IHC
ICC
FC
ELISA
ChIP
1:500-1:1000
-
1:200
1:50
抗体详细信息如下:
About this Antibody
Name
Category
Polyclonal antibody(pAb); Primary antibody
Isotype
IgG
Purification
Peptide XYfinity purified
About the Immunogen
Immunogen
This antibody is produced by immunizing rabbits with a synthetic peptide (KLH-coupled) corresponding to a region of N-cadherin.
Gene ID
1000
SwissProt
P19022
Synonyms
CDH2; CDCN; NCAD
Adhesion/ECM
Background
The cadherins are a large family of cell adhesion molecules involved in calcium-dependent recognition and adhesion events. The cadherin subfamily includes N-, P-, B-, and E- cadherins, and other members. Recent studies indicate that cancer cells have up-regulated N-cadherin in addition to loss of E-cadherin. N-cadherin cooperates with the FGF receptor, leading to overexpression of MMP-9 and cellular invasion. N-Cadherin is commonly found in cancer cells and provides a mechanism for transendothelial migration. When a cancer cell adheres to the endothelial cells of a blood vessel it up-regulates the src kinase pathway, which phosphorylates beta-catenins attached to both N-cadherin (this protein) and E-cadherins.
包装清单:
包装
AZ050
Western一抗稀释液
50ml
—
说明书
1份
保存条件: N-Cadherin Rabbit Polyclonal Antibody -20℃保存,Western一抗稀释液-20℃或4℃保存,一年有效。Western一抗稀释液优先推荐4℃保存,长期不使用可以考虑-20℃保存,但冻融可能会导致出现轻微的浑浊和少量不溶物。 注意事项: 如果本抗体用于Western blot (WB)、**荧光(IF)、**细胞化学(ICC)等实验,请注意回收使用过的稀释抗体。回收的抗体通常至少可以重复使用5-10次。稀释后的抗体,包括已经使用过的稀释抗体,请4℃保存。 回收后重复使用的抗体,使用方法同新鲜稀释的抗体。如果在重复使用过程中发现抗体出现轻微混浊现象,可以10,000g离心1-3分钟,取上清用于后续检测。如果回收的抗体出现明显的絮状物或长霉长菌等情况,则可以考虑废弃该抗体。 提供的Western一抗稀释液也可以用于**荧光(IF)、**组化(IHC)、**细胞化学(ICC)等适当用途。如果希望获得*佳的检测效果,请考虑使用上述检测专用的一抗稀释液。 本产品**于专业人员的科学研究用,不得用于临床诊断或**,不得用于食品或药品,不得存放于普通住宅内。 为了您的**和健康,请穿实验服并戴一次性手套操作。