Phospho-Akt1 (Ser473) Rabbit Monoclonal Antibody
产品编号
产品名称
产品包装
产品价格
XY1546
50μl
618.00元
来源
用途
交叉反应性
分子量
Rabbit
WB, IF, IHC, ICC
H, M
56.0kDa
WB, Western blot; IP, Immunoprecipitation; IF, Immunofluorescence; IHC, Immunohistochemistry; ICC, Immunocytochemistry; FC, Flow Cytometry; ELISA, Enzyme-linked Immunosorbent Assay; ChIP, Chromatin Immunoprecipitation Assay. H, Human; M, Mouse; R, Rat; C, Chicken; Cw, Cow; Dg, Dog; Gp, Guinea pig; Hm, Hamster; Hr, Horse; Mk, Monkey; Pg, Pig; Rb, Rabbit; S, Sheep; Z, ZebrXYish; All, all species expected. 配套提供了Western一抗稀释液，可以用于Western检测或其它适当用途时的一抗稀释。 建议抗体使用时的稀释比例如下(实际使用时需根据抗原水平的高低作适当调整)：
WB
IP
IF
IHC
ICC
FC
ELISA
ChIP
1:1,000-1:2,000
-
1:50-1:200
抗体详细信息如下:
About this Antibody
Name
Category
Rabbit Monoclonal Antibody (RabMAb); Primary antibody
Isotype
IgG
Purification
XYfinity purification
About the Immunogen
Immunogen
Recombinant protein
Gene ID
207(Human); 11651(Mouse)
SwissProt
P31749(Human); P31750(Mouse)
Synonyms
AKT; CWS6; PKB; PKB-ALPHA; PRKBA; RAC; RAC-ALPHA
PI3K/Akt/mTOR
Background
The serine/threonine kinase Akt family contains several members, including Akt1 (also designated PKB or RacPK), Akt2 (also designated PKBβ or RacPK-β) and Akt 3 (also designated PKBγ or thyoma viral proto-oncogene 3), which exhibit sequence homology with the protein kinase A and C families and are encoded by the c-Akt proto-oncogene. All members of the Akt family have a pleckstrin homology domain. Akt1 and Akt2 are activated by PDGF stimulation. This activation is dependent on PDGFR-β tyrosine residues 740 and 751, which bind the subunit of the phosphatidylinositol 3-kinase (PI 3-kinase) complex. Activation of Akt1 by insulin or insulin-growth factor-1(IGF-1) results in phosphorylation of both Thr 308 and Ser 473. Phosphorylation of both residues is important to generate a high level of Akt1 activity, and the phosphorylation of Thr 308 is not dependent on phosphorylation of Ser 473 in vivo. Thus, Akt proteins become phosphorylated and activated in insulin/IGF-1-stimulated cells by an upstream kinase(s). The activation of Akt1 and Akt2 is inhibited by the PI kinase inhibitor wortmannin, suggesting that the protein signals downstream of the PI kinases.
包装清单:
包装
XY050
Western一抗稀释液
50ml
说明书
1份
保存条件: Phospho-Akt1 (Ser473) Rabbit Monoclonal Antibody -20℃保存，Western一抗稀释液-20℃或4℃保存，一年有效。Western一抗稀释液优先推荐4℃保存，长期不使用可以考虑-20℃保存，但冻融可能会导致出现轻微的浑浊和少量不溶物。 注意事项: 如果本抗体用于Western blot (WB)、**荧光(IF)、**细胞化学(ICC)等实验，请注意回收使用过的稀释抗体。回收的抗体通常至少可以重复使用5-10次。稀释后的抗体，包括已经使用过的稀释抗体，请4℃保存。 回收后重复使用的抗体，使用方法同新鲜稀释的抗体。如果在重复使用过程中发现抗体出现轻微混浊现象，可以10,000g离心1-3分钟，取上清用于后续检测。如果回收的抗体出现明显的絮状物或长霉长菌等情况，则可以考虑废弃该抗体。 提供的Western一抗稀释液也可以用于**荧光(IF)、**组化(IHC)、**细胞化学(ICC)等适当用途。如果希望获得*佳的检测效果，请考虑使用上述检测专用的一抗稀释液。 本产品**于专业人员的科学研究用，不得用于临床诊断或**，不得用于食品或药品，不得存放于普通住宅内。 为了您的**和健康，请穿实验服并戴一次性手套操作。