B Raf Rabbit Monoclonal Antibody
产品编号
产品名称
产品包装
产品价格
XY1621
B RXY Rabbit Monoclonal Antibody
50μl
618.00元
来源
用途
交叉反应性
分子量
Rabbit
WB, IP, IHC, FC
H, M, R
85.0kDa
WB, Western blot; IP, Immunoprecipitation; IF, Immunofluorescence; IHC, Immunohistochemistry; ICC, Immunocytochemistry; FC, Flow Cytometry; ELISA, Enzyme-linked Immunosorbent Assay; ChIP, Chromatin Immunoprecipitation Assay. H, Human; M, Mouse; R, Rat; C, Chicken; Cw, Cow; Dg, Dog; Gp, Guinea pig; Hm, Hamster; Hr, Horse; Mk, Monkey; Pg, Pig; Rb, Rabbit; S, Sheep; Z, ZebrXYish; All, all species expected. 配套提供了Western一抗稀释液，可以用于Western检测或其它适当用途时的一抗稀释。 建议抗体使用时的稀释比例如下(实际使用时需根据抗原水平的高低作适当调整)：
WB
IP
IF
IHC
ICC
FC
ELISA
ChIP
1:1,000
1:10-1:50
-
1:50-1:200
1:50-1:100
抗体详细信息如下:
About this Antibody
Name
Category
Rabbit Monoclonal Antibody (RabMAb); Primary antibody
Isotype
IgG
Purification
XYfinity purification
About the Immunogen
Immunogen
Recombinant protein
Gene ID
673(Human); 109880(Mouse); 114486(Rat)
SwissProt
P15056(Human); P28028(Mouse);
Synonyms
B-RXY1; BRXY1; NS7; RXYB1
MAPK
Background
Several serine/threonine protein kinases have been implicated as intermediates in signal transduction pathways. These include ERK/MAP kinases, ribosomal S6 kinase (Rsk) and RXY-1. RXY-1 is a cytoplasmic protein with intrinsic serine/threonine activity. It is broadly expressed in nearly all cell lines tested to date and is the cellular homolog of v-RXY, the product of the transforming gene of the 3611 strain of murine sarcoma virus. The unregulated kinase activity of the v-RXY protein has been associated with transformation and mitogenesis, while the activity of RXY-1 is normally suppressed by a regulatory N-terminal domain. RXY-A, a second member of the RXY gene family of serine/threonine protein kinases, exhibits substantial homology to RXY-1 within the kinase domain of the two molecules, but less homology elsewhere. Expression of RXY-B is highly restricted, with highest levels in the cerebrum and testis.
包装清单:
包装
XY050
Western一抗稀释液
50ml
说明书
1份
保存条件: B RXY Rabbit Monoclonal Antibody -20℃保存，Western一抗稀释液-20℃或4℃保存，一年有效。Western一抗稀释液优先推荐4℃保存，长期不使用可以考虑-20℃保存，但冻融可能会导致出现轻微的浑浊和少量不溶物。 注意事项: 如果本抗体用于Western blot (WB)、**荧光(IF)、**细胞化学(ICC)等实验，请注意回收使用过的稀释抗体。回收的抗体通常至少可以重复使用5-10次。稀释后的抗体，包括已经使用过的稀释抗体，请4℃保存。 回收后重复使用的抗体，使用方法同新鲜稀释的抗体。如果在重复使用过程中发现抗体出现轻微混浊现象，可以10,000g离心1-3分钟，取上清用于后续检测。如果回收的抗体出现明显的絮状物或长霉长菌等情况，则可以考虑废弃该抗体。 提供的Western一抗稀释液也可以用于**荧光(IF)、**组化(IHC)、**细胞化学(ICC)等适当用途。如果希望获得*佳的检测效果，请考虑使用上述检测专用的一抗稀释液。 本产品**于专业人员的科学研究用，不得用于临床诊断或**，不得用于食品或药品，不得存放于普通住宅内。 为了您的**和健康，请穿实验服并戴一次性手套操作。