AMPK alpha 1 Rabbit Monoclonal Antibody
产品编号
产品名称
产品包装
产品价格
XY1627
50μl
618.00元
来源
用途
交叉反应性
分子量
Rabbit
WB, IP, IF, ICC, FC
H, M, R
63.0kDa
WB, Western blot; IP, Immunoprecipitation; IF, Immunofluorescence; IHC, Immunohistochemistry; ICC, Immunocytochemistry; FC, Flow Cytometry; ELISA, Enzyme-linked Immunosorbent Assay; ChIP, Chromatin Immunoprecipitation Assay. H, Human; M, Mouse; R, Rat; C, Chicken; Cw, Cow; Dg, Dog; Gp, Guinea pig; Hm, Hamster; Hr, Horse; Mk, Monkey; Pg, Pig; Rb, Rabbit; S, Sheep; Z, ZebrXYish; All, all species expected. 配套提供了Western一抗稀释液，可以用于Western检测或其它适当用途时的一抗稀释。 建议抗体使用时的稀释比例如下(实际使用时需根据抗原水平的高低作适当调整)：
WB
IP
IF
IHC
ICC
FC
ELISA
ChIP
1:1,000-1:5,000
1:10-1:50
1:50-1:200
-
1:50-1:100
抗体详细信息如下:
About this Antibody
Name
Category
Rabbit Monoclonal Antibody (RabMAb); Primary antibody
Isotype
IgG
Purification
XYfinity purification
About the Immunogen
Immunogen
Recombinant protein
Gene ID
5562(Human); 105787(Mouse); 65248(Rat)
SwissProt
Q13131(Human); Q5EG47(Mouse); P54645(Rat)
Synonyms
PRKAA1; AMPK; AMPKa1
Cell Cycle
Background
AMPK (for 5'-AMP-activated protein kinase) is a heterotrimeric complex com-prising a catalytic α subunit and regulatory β and γ subunits. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming bio-synthetic pathways. AMPK is activated by high AMP and low ATP through a mechanism involving allosteric regulation, promotion of phosphorylation by an upstream protein kinase known as AMPK kinase, and inhibition of dephosphorylation. Activated AMPK can phosphorylate and regulate in vivo hydroxy-methylglutaryl-CoA reductase and acetyl-CoA carboxylase, which are key regulatory enzymes of sterol synthesis and fatty acid synthesis, respectively. The human AMPKα1 and AMPKα2 genes encode 548 amino acid and 552 amino acid proteins, respectively. Human AMPKβ1 encodes a 271 amino acid protein and human AMPKβ2 encodes a 272 amino acid protein. The human AMPKγ1 gene encodes a 331 amino acid protein. Human AMPKγ2 and AMPKγ3, which are 569 and 492 amino acid proteins, respectively, contain unique N-terminal domains and may participate directly in the binding of AMP within the AMPK complex.
包装清单:
包装
XY050
Western一抗稀释液
50ml
说明书
1份
保存条件: AMPK alpha 1 Rabbit Monoclonal Antibody -20℃保存，Western一抗稀释液-20℃或4℃保存，一年有效。Western一抗稀释液优先推荐4℃保存，长期不使用可以考虑-20℃保存，但冻融可能会导致出现轻微的浑浊和少量不溶物。 注意事项: 如果本抗体用于Western blot (WB)、**荧光(IF)、**细胞化学(ICC)等实验，请注意回收使用过的稀释抗体。回收的抗体通常至少可以重复使用5-10次。稀释后的抗体，包括已经使用过的稀释抗体，请4℃保存。 回收后重复使用的抗体，使用方法同新鲜稀释的抗体。如果在重复使用过程中发现抗体出现轻微混浊现象，可以10,000g离心1-3分钟，取上清用于后续检测。如果回收的抗体出现明显的絮状物或长霉长菌等情况，则可以考虑废弃该抗体。 提供的Western一抗稀释液也可以用于**荧光(IF)、**组化(IHC)、**细胞化学(ICC)等适当用途。如果希望获得*佳的检测效果，请考虑使用上述检测专用的一抗稀释液。 本产品**于专业人员的科学研究用，不得用于临床诊断或**，不得用于食品或药品，不得存放于普通住宅内。 为了您的**和健康，请穿实验服并戴一次性手套操作。