小鼠抗聚组氨酸单克隆抗体
规格:1mg/1ml
英文名: His tag(2D5)
别名: HisX6; 6His; His Tag; 6 His epitope tag; Hexa His tag; HHHHHH epitope tag; HHHHHH tag; Histidine.
储存液:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glyce
克隆类型:Monoclonal
亚型:IgG
纯化方法:affinity purified by Protein A
**原:KLH conjugated HHHHHH peptide
交叉反应:fusionprotein,HHHHHHtagged
小鼠抗聚组氨酸单克隆抗体细胞定位:
产品介绍:background: The H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. The antibody recognizes the His-tag fused to the amino- or carboxy- termini of targeted proteins in transfected or transformed cells. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.小鼠抗聚组氨酸单克隆抗体 蛋白质纯化的新途径─应用His-Tag抗体检测His-Tag融合蛋白 1) 6xHis与不同的Sepharose纯化填料结合(捆绑),可不受构象的影响,在天然或变形的条件下可进行进一步纯化。 2) 可在温和的洗脱液环境中,其结合、洗涤及洗脱步骤不会对蛋白结构产生影响。 3) 6xHis标签比普通(Tag)要小得多,经纯化后的蛋白可直接进行下游操作。可用于任何表达系统(包括: **,杆状病毒和哺乳动物) 4小鼠抗聚组氨酸单克隆抗体) 6xHis标签在生理溶液pH下不带电荷,其标签不会干涉重组蛋白的结构和功能, 6xHis不会影响蛋白分泌。 5) 6xHis标签**原性差,重组蛋白可以不必除去标签而直接作为抗原进行**。
小鼠抗聚组氨酸单克隆抗体产品应用:WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 (石蜡切片需做抗原修复) not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
研究领域:细胞生物 **学
储存条件: Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
来源: Mouse
外观: Lyophilized or Liquid