5-溴脱氧尿嘧啶核苷/增殖标志物单克隆抗体
规格：1mg/1ml
英文名： BrdU
别名： Bromodeoxyuridine; Bromodeoxyuridine; 5-Bromo-2′-deoxyuridine; 5-BrdU; Proliferation Marker; 5-Bromo-2-deoxyUridine.
分子量： 0.3071kDa
储存液：0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glyce
克隆类型：Monoclonal
亚型：IgG
纯化方法：affinity purified by Protein G
**原：bromodeoxyuridine conjugated to KLH
交叉反应：BrdU
5-溴脱氧尿嘧啶核苷/增殖标志物单克隆抗体细胞定位：细胞核
产品介绍：background: Proliferation Marker Bromodeoxyuridine (BrdU) is a thymidine analog and is specifically incor-porated into DNA during DNA synthesis. Anti-bromodeoxyuridine monoclonal antibody is used to identify cells that have incorporated BrdU. This immunological detection scheme has several advantages over the use of radioactive thymidine incorporation for identifying cells under-going replication. Labeling and detection can be performed the same day instead of waiting several days, as required for autoradiography of tritium-labeled cells, and the necessity 5-溴脱氧尿嘧啶核苷/增殖标志物单克隆抗体of using multiple specimens for obtaining the optimal exposure time is eliminated. In addition, anti-bromodeoxyuridine staining with flow cytometric analysis allows multiple parameters to be evaluated simultaneously. Anti-bromodeoxyuridine monoclonal antibody has been used for identi-fying proliferating cells in blood (Campana et al., 1988), tissues (Schutte et al., 1987; Hayashi, et al., 1988),5-溴脱氧尿嘧啶核苷/增殖标志物单克隆抗体 tumors (Hoshino et al., 1986; Morstyn et al., 1983), as well as for determining plasma cell labeling indices (Greipp et al., 1985). Subcellular Location: Nuclear. CAS: 59-14-3 Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 增殖标志物 BrdU（溴化脱氧尿嘧啶核苷）可以在体内和体外掺入到处于S期的细胞所合成的DNA链中。此抗体可以与掺入到任何种属细胞中的BrdU反应，与碘脱氧尿嘧啶有交叉反应，标记掺有BrdU的S期细胞，主要用于研究各种不同的因素对正常/肿瘤组织的细胞增殖及动力学的研究。 用FITC标记的抗BrdU IgG可用于流式细胞术定量检测增值细胞。
5-溴脱氧尿嘧啶核苷/增殖标志物单克隆抗体产品应用：ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
研究领域：细胞生物 **学 发育生物学 染色质和核信号 神经生物学 **及化合物 细胞类型标志物
储存条件： Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
来源： Mouse
外观： Lyophilized or Liquid