蛋白质磷酸酶-2A抗体
规格：1mg/1ml
英文名： PP2A alpha + beta
别名： PPP2CA; PP2A alpha; PP-2A; PP2A C; PP2Ac; PP2CA; PPP2CA; Protein phosphatase 2, catalytic subunit, alpha isoform; Replication protein C; RP C; PP2AA_HUMAN.
分子量： 34kDa
储存液：0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glyce
克隆类型：Polyclonal
亚型：IgG
纯化方法：affinity purified by Protein A
**原：KLH conjugated synthetic peptide derived from human PP-2A C-
交叉反应：Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Rabbit,
蛋白质磷酸酶-2A抗体细胞定位：细胞核 细胞浆
产品介绍：background: This gene encodes the phosphatase 2A catalytic subunit. Protein phosphatase 2A is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. This gene encodes an alpha isoform of the catalytic subunit. [provided by RefSeq, Jul 2008]. This antibody is crosed with PP-2A subunit A,B. Function: PP2A can modulate the activity of phosphorylase B kinase casein kinase 2, mitogen-stimulated S6 kinase, and MAP-2 kinase. Cooperates with SGOL2 to protect centromeric cohesin from separase-mediated cleavage in oocytes specifically during meiosis I (By similarity). Can dephosphorylate SV40 large T antigen and p53/TP53. Dephosphorylates SV40 large T antigen, preferentially on serine residues 120, 123, 677, and perhaps 679. The C subunit was most active, followed by the AC form, which was more active than the ABC form, and activity of all three forms was strongly stimulated by manganese, and to a lesser extent by magnesium. Dephosphorylation by the AC form, but not C or ABC form is inhibited by small T antigen. Activates RAF1 by dephosphorylating it at 'Ser-259'蛋白质磷酸酶-2A抗体. Subunit: PP2A consists of a common heterodimeric core enzyme, composed of PPP2CA a 36 kDa catalytic subunit (subunit C) and PPP2R1A a 65 kDa constant regulatory subunit (PR65 or subunit A), that associates with a variety of regulatory subunits. Proteins that associate with the core dimer include three families of regulatory subunits B (the R2/B/PR55/B55, R3/B''/PR72/PR130/PR59 and R5/B'/B56 families), the 48 kDa variable regulatory subunit, viral proteins, and cell signaling molecules. Interacts with NXN; the interaction is direct (By similarity). Interacts with TP53, SGOL1 and SGOL2. Interacts with AXIN1; the interaction dephosphorylates AXIN1. Interacts with PIM3; this interaction promotes dephosphorylation, ubiquitination and proteasomal degradation of PIM3. Interacts with RAF1. Subcellular Location: Cytoplasm. Nucleus. Chromosome, centromere. Cytoplasm, 蛋白质磷酸酶-2A抗体cytoskeleton, spindle pole. Note=In prometaphase cells, but not in anaphase cells, localizes at centromeres. During mitosis, also found at spindle poles. Centromeric localization requires the presence of SGOL2 (By similarity). Post-translational modifications: Reversibly methyl esterified on Leu-309. Carboxyl methylation may play a role in holoenzyme assembly, enhancing the affinity of the PP2A core enzyme for some, but not all, regulatory subunits. It varies during the cell cycle. Phosphorylation of either threonine (by autophosphorylation-activated protein kinase) or tyrosine results in inactivation of the phosphatase. Auto-dephosphorylation has been suggested as a mechanism for reactivation. Similarity: Belongs to the PPP phosphatase family. PP-1 subfamily. Gene ID: 5515 Database links: Entrez Gene: 416318 Chicken Entrez Gene: 282320 Cow Entrez Gene: 5515 Human Entrez Gene: 19052 Mouse Entrez Gene: 397656 Pig Entrez Gene: 100009252 Rabbit Entrez Gene: 24672 Rat Omim: 176915 Human SwissProt: P48463 Chicken SwissProt: P67774 Cow SwissProt: P67775 Human SwissProt: P63330 Mouse SwissProt: P67776 Pig SwissProt: P67777 Rabbit SwissProt: P63331 Rat Unigene: 105818 Human Unigene: 260288 Mouse Unigene: 1271 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 激酶和磷酸酶（Kinases and Phosphatases） PP-2A (protein phosphatase 2A catalytic subunit；PP2A alpha；)参与酵母细胞及两栖类卵母细胞有丝分裂的蛋白丝/苏氨酸磷酸酶。 此酶的表达与细胞周期调节有关。此抗体与PP-2A subunit A,B,C 均有交叉反应。
蛋白质磷酸酶-2A抗体产品应用：WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
研究领域：细胞生物 **学 信号转导 细胞周期蛋白 激酶和磷酸酶
储存条件： Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
来源： Rabbit
外观： Lyophilized or Liquid
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