间变型**瘤激酶抗体

间变型**瘤激酶抗体

规格：1mg/1ml

英文名： ALK

别名： ALK tyrosine kinase receptor; ALK tyrosine kinase receptor precursor; ALK/EML4 fusion gene, included; ALK/NPM1 fusion gene, included; anaplastic lymphoma kinase (Ki-1); Anaplastic lymphoma kinase; Ana

分子量： 174kDa

储存液：0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glyce

克隆类型：Polyclonal

亚型：IgG

纯化方法：affinity purified by Protein A

**原：KLH conjugated synthetic peptide derived from human CD246 N-

交叉反应：Human, Mouse, Rat, Dog, Cow, Horse,

间变型**瘤激酶抗体细胞定位：细胞膜

产品介绍：background: This gene encodes a receptor tyrosine kinase, which belongs to the insulin receptor superfamily. This protein comprises an extracellular domain, an hydrophobic stretch corresponding to a single pass transmembrane region, and an intracellular kinase domain. It plays an important role in the development of the brain and exerts its effects on specific neurons in the nervous system. This gene has been found to be rearranged, mutated, or amplified in a series of tumours including anaplastic large cell lymphomas, neuroblastoma, and non-small cell lung cancer. The chromosomal rearrangements are the most common genetic alterations in this gene, which result in creation of multiple fusion genes in tumourigenesis, including ALK (chromosome 2)/EML4 (chromosome 2), ALK/RANBP2 (chromosome 2), ALK/ATIC (chromosome 2), ALK/TFG (chromosome 3), ALK/NPM1 (chromosome 5), ALK/SQSTM1 (chromosome 5), LK/KIF5B (chromosome 10), ALK/CLTC (chromosome 17), ALK/TPM4 (chromosome 19), and ALK/MSN (chromosome X).[provided by RefSeq, Jan 2011]. Function: Neuronal orphan receptor tyrosine kinase that is essentially and transiently expressed in specific regions of the central and peripheral nervous systems and plays an important role in the genesis and differentiation of the nervous system. Transduces signals from ligands at the cell surface, through specific activation of the mitogen-activated protein kinase (MAPK) pathway. Phosphorylates almost exclusively at the first tyrosine of the Y-x-x-x-Y-Y motif. Following activation by ligand, ALK induces tyrosine phosphorylation of CBL, FRS2, IRS1 and SHC1, as well as of the MAP kinases MAPK1/ERK2 and MAPK3/ERK1. Acts as a receptor for ligands pleiotrophin (PTN), a间变型**瘤激酶抗体 secreted growth factor, and midkine (MDK), a PTN-related factor, thus participating in PTN and MDK signal transduction. Subunit: Homodimer. Homodimerizes when bound to ligand. Interacts with FRS2, IRS1, MDK, PTN and SHC1. Interacts with CBL, PIK3R1 and PLCG1. Subcellular Location: Cell membrane; Single-pass type I membrane protein. Note=Membrane attachment was crucial for promotion of neuron-like differentiation and cell proliferation arrest through specific activation of the MAP kinase pathway. Tissue Specificity: Expressed in brain and CNS. Also expressed in the small intestine and testis, but not in normal lymphoid cells. Post-translational modifications: Phosphorylated at tyrosine residues by autocatalysis, which activates kinase activity. In cells not stimulated by a ligand, receptor protein tyrosine phosphatase beta and zeta complex (PTPRB/PTPRZ1) dephosphorylates ALK at the sites in ALK that are undergoing autophosphorylation through autoactivation. N-glycosylated. DISEASE: Note=A chromosomal aberration involving ALK is found in a form of non-Hodgkin lymphoma. Translocation t(2;5)(p23;q35) with NPM1. The resulting chimeric NPM1-ALK protein homodimerize and the kinase becomes constitutively activated. The constitutively active fusion proteins are responsible for 5-10% of non-Hodgkin Note=A chromosomal aberration involving ALK is associated with inflammatory myofibroblastic tumors (IMTs). Translocation t(2;11)(p23;p15)间变型**瘤激酶抗体 with CARS; translocation t(2;4)(p23;q21) with SEC31A. Similarity: Belongs to the protein kinase superfamily. Tyr protein kinase family. Insulin receptor subfamily. Contains 1 LDL-receptor class A domain. Contains 2 MAM domains. Contains 1 protein kinase domain. Gene ID: 238 Database links: Entrez Gene: 238 Human Entrez Gene: 11682 Mouse Entrez Gene: 266802 Rat Omim: 105590 Human SwissProt: Q9UM73 Human SwissProt: P97793 Mouse Unigene: 654469 Human Unigene: 311854 Mouse Unigene: 201918 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. ALK蛋白在细胞生长调控中起重要作用，主要表达在神经细胞,白细胞癌基因蛋白。 ALK p80是多向性生长因子酪氨酸激酶受体蛋白， 间变性大细胞**瘤伴有t(2;5) (p23;q35)染色体的易位，易位后的细胞基因表达分子量为80kD，常伴有CD30阳性，ALK p80阳性的间变性大细胞**瘤预后好于阴性病例。 主要用于间变性大细胞**瘤与何杰金氏**瘤的鉴别诊断，间变性大细胞**瘤中阳性率大约为70%左右，还可以用于骨髓中间变性大细胞**瘤的诊断.

间变型**瘤激酶抗体产品应用：ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1:20-500 IF=1:100-500 （石蜡切片需做抗原修复） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.

研究领域：**学  神经生物学  信号转导  生长因子和**  转录调节因子  激酶和磷酸酶  细胞类型标志物  b-**细胞  肿瘤细胞生物标志物  

储存条件： Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

来源： Rabbit

外观： Lyophilized or Liquid

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