小鼠抗丙酮酸激酶-M2抗体
规格：1mg/1ml
英文名： PKM2
别名： PK 1; PK 2; PK 3; PK Muscle type; PK1; PK2; Pk3; PKL; PKLR; PKM 2; PKM; PKM2; PYKM; Pyruvate kinase 1; Pyruvate kinase 2/3; Pyruvate kinase 3; Pyruvate kinase isozyme R/L; Pyruvate kinase isozymes M1/
分子量： 58kDa
储存液：0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glyce
克隆类型：Polyclonal
亚型：IgG
纯化方法：affinity purified by Protein A
**原：KLH conjugated synthetic peptide derived from human PKM2
交叉反应：Human, Mouse, Rat, Pig, Cow, Horse, Rabbit,
小鼠抗丙酮酸激酶-M2抗体细胞定位：细胞核 细胞浆
产品介绍：background: The protein encoded by this gene is a pyruvate kinase that catalyzes the production of phosphoenolpyruvate from pyruvate and ATP. This protein has been shown to interact with thyroid hormone, and thus may mediate cellular metabolic effects induced by thyroid hormones. This protein has been found to bind Opa protein, a bacterial outer membrane protein involved in gonococcal adherence to and invasion of human cells, suggesting a role of this protein in bacterial pathogenesis. Three alternatively spliced transcript variants encoding two distinct isoforms have been reported. Function: Glycolytic enzyme that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP. Stimulates POU5F1-mediated transcriptional activation. Plays a general role in caspase independent cell death of tumor cells. The ratio betwween the highly active tetrameric form and nearly inactive dimeric form determines whether glucose carbons are channeled to biosynthetic processes or used for glycolytic ATP production. The transition between the 2 forms contributes to the control of glycolysis and is important for tumor小鼠抗丙酮酸激酶-M2抗体 cell proliferation and survival. Subunit: Monomer and homotetramer. Exists as a monomer in the absence of FBP, and reversibly associates to form a homotetramer in the presence of FBP. The monomeric form binds T3. Tetramer formation induces pyruvate kinase activity. The tetrameric form has high affinity for the substrate and is associated within the glycolytic enzyme complex. Exists in a nearly inactive dimeric form in tumor cells and the dimeric form has less affinity for the substrate. Binding to certain oncoproteins such as HPV-16 E7 oncoprotein can trigger dimerization. FBP stimulates the formation of tetramers from dimers. Interacts with HERC1, POU5F1 and PML. Interacts (isoform M2) with EGLN3; the interaction hydroxylates PKM under hypoxia and enhances binding to HIF1A. Interacts (isoform M2) with HIF1A; the interaction is enhanced by binding of EGLN3, promoting enhanced transcription activity under hypoxia. Subcellular Location: Cytoplasm. Nucleus. Note=Translocates to the nucleus in response to different apoptotic stimuli. Nuclear translocation is sufficient to induce cell death that is caspase independent, isoform-specific and independent of its enzymatic activity. Tissue Specificity: Specifically expressed in proliferating cells, such as embryonic stem cells, embryonic carcinoma cells, as well as cancer cells. Post-translational modifications: ISGylated. Under hypoxia, hydroxylated by EGLN3. Acetylation at Lys-305 is stimulated by high glucose concentration, it decreases enzyme activity and promotes its lysosomal-dependent degradation via chaperone-mediated autophagy. Similarity: 小鼠抗丙酮酸激酶-M2抗体Belongs to the pyruvate kinase family. Gene ID: 5315 Database links: Entrez Gene: 5315 Human Entrez Gene: 18746 Mouse Entrez Gene: 25630 Rat Omim: 179050 Human SwissProt: P14618 Human SwissProt: P52480 Mouse SwissProt: P11980 Rat Unigene: 534770 Human Unigene: 326167 Mouse Unigene: 405069 Mouse Unigene: 1556 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 丙酮酸激酶（PK）有L、R、M1、M2四种同功酶，均为四聚体。 L型主要分布于肝脏，R型存在于红细胞，在结构、**学和动力学上十分相似，由同一基因调控；M1存在于肌肉中，M2分布于除上述以外的其他组织（尤以肾脏*多）以及胎儿各组织。PK也是一种癌胚蛋白，在恶性肿瘤中，增高的都是M2型。
小鼠抗丙酮酸激酶-M2抗体产品应用：WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
研究领域：肿瘤 **学 信号转导 细胞周期蛋白 激酶和磷酸酶 肿瘤细胞生物标志物 新陈代谢
储存条件： Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
来源： Mouse
外观： Lyophilized or Liquid