重组金黄色葡萄球菌蛋白A抗体
规格:1mg/1ml
英文名: Protein A
别名: Immunoglobulin G binding protein A; Staphylococcal protein A; SPA; gG-binding protein A.
分子量: 40-60kDa
储存液:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glyce
克隆类型:Polyclonal
亚型:IgG
纯化方法:affinity purified by Protein A
**原:Recombinant protein A
交叉反应:Saureus
重组金黄色葡萄球菌蛋白A抗体细胞定位:分泌型蛋白
产品介绍:background: Protein A is a 40-60 kDa surface protein originally found in the cell wall of the bacteria Staphylococcus aureus (SPA). SPA binds proteins from many of mammalian species, most notably IgGs, and helps inhibit phagocytic engulfment and acts as an immunological disguise via this type of interaction, thus the bacteria will disrupts opsonization and phagocytosis. SPA is known to bind with Fc region of immunoglobulins preferentially through interaction with the VH3 variable region of the heavy chain. SPA has been shown to bind with high affinity to 重组金黄色葡萄球菌蛋白A抗体human IgG1 and IgG2 as well as mouse IgG2a and IgG2b, whereas bind with moderate affinity to human IgM, IgA and IgE as well as mouse IgG3 and IgG1. SPA is often produced in E. coli and is practically coupled to other molecules such as enzymes, biotin, radioactive iodine for use in immunology and other biological research. SPA is also immobilized onto solid supports such as agarose 重组金黄色葡萄球菌蛋白A抗体beads for total IgG purifying or interest protein or protein complex identifying in immunoprecipitation studies. Subcellular Location: Secreted; cell wall. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
重组金黄色葡萄球菌蛋白A抗体产品应用:WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 (石蜡切片需做抗原修复) not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
研究领域:**学
储存条件: Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
来源: Rabbit
外观: Lyophilized or Liquid