金属蛋白酶组织抑制因子-4抗体
规格：1mg/1ml
英文名： TIMP-4
别名： Metalloproteinase inhibitor 4; TIMP 4; TIMP4; TIMP metallopeptidase inhibitor 4; TIMP-4; Timp4; TIMP4_HUMAN; Tissue inhibitor of metalloproteinase 4.
分子量： 22kDa
储存液：0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glyce
克隆类型：Polyclonal
亚型：IgG
纯化方法：affinity purified by Protein A
**原：KLH conjugated synthetic peptide derived from human TIMP-4
交叉反应：Human, Mouse, Rat, Cow, Horse, Rabbit,
金属蛋白酶组织抑制因子-4抗体细胞定位：
产品介绍：background: The tissue inhibitors of metalloproteinases (TIMPs) are naturally occurring proteins that specifically inhibit matrix metalloproteinases and regulate extracellular matrix turnover and tissue remodeling by forming tightbinding inhibitory complexes with the MMPs. Thus, TIMPs maintain the balance between matrix destruction and formation. An imbalance between MMPs and the associated TIMPs may play a significant role in the invasive phenotype of malignant tumors. The TIMP proteins share several structural features including six loops held in place by six disulfide bonds arranged in three knot-like structures. These proteins also contain twelve cysteine residues in conserved regions of the molecule that form six disulfide bonds, essential for the formation of native conformations, and the N terminal region that is necessary for inhibitory activities. The N terminus of each TIMP contains a consensus sequence (VIRAK) and each TIMP is translated with a 29 amino acid leader sequence that is cleaved off to produce the mature protein. The C terminal regions are divergent, which enhances the selectivity of inhibition and binding efficiency. Although the TIMP proteins share high homology, they may either be secreted extracellularly in soluble form (TIMP1, TIMP2 and TIMP4) or bind to extracellular matrix components (TIMP3). The MMPs and TIMPs can be divided into two groups with respect to gene expression: the majority exhibit inducible expression and a small number are produced constitutively or are expressed at very low levels and are not inducible. Among agents that induce MMP and TIMP production are the inflammatory cytokines TNF alpha and IL1 beta. A marked cell type specificity is a hallmark of both MMP and金属蛋白酶组织抑制因子-4抗体 TIMP gene expression (i.e. a limited number of cell types can be induced to make these proteins). Tissue Inhibitor of Metalloproteinases 4 (TIMP4) was identified by molecular cloning. TIMP4 shows 37 % amino acid identity with TIMP1 and 51 % homology with TIMP2 and TIMP3. TIMP4 is secreted extracellularly, predominantly in heart and brain tissue. It may function in a tissue specific fashion in extracellular matrix (ECM) homeostasis. TIMP4 has a strong inhibitory effect on the invasion of human breast cancer cells across reconstituted basement membranes suggesting that TIMP4 may have an important role in inhibiting primary tumor growth and progression. The human TIMP4 gene has the chromosomal location of 3p25. Function: Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor. 金属蛋白酶组织抑制因子-4抗体Known to act on MMP-1, MMP-2, MMP-3, MMP-7 and MMP-9. Subcellular Location: Secreted. Tissue Specificity: Abundant in heart and present at low levels in many other tissues. Similarity: Belongs to the protease inhibitor I35 (TIMP) family. Contains 1 NTR domain. Gene ID: 7079 Database links: Entrez Gene: 7079 Human Entrez Gene: 110595 Mouse Entrez Gene: 114849 Rat Entrez Gene: 680130 Rat Omim: 601915 Human SwissProt: Q99727 Human SwissProt: Q9JHB3 Mouse SwissProt: P81556 Rat Unigene: 591665 Human Unigene: 255607 Mouse Unigene: 155651 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 合成与降解（Synthesis and Degradation） TIMP-4是TIMP家族的新成员，TIMP-3与TIMP-4同样具有较强的促凋亡、抑制血管生成、抗肿瘤浸润作用。
金属蛋白酶组织抑制因子-4抗体产品应用：WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
研究领域：肿瘤 心血管 神经生物学 合成与降解
储存条件： Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
来源： Rabbit
外观： Lyophilized or Liquid