5-溴脱氧尿嘧啶核苷抗体
规格：1mg/1ml
英文名： BrdU
别名： Bromodeoxyuridine; Bromodeoxyuridine; 5-Bromo-2′-deoxyuridine; 5-BrdU; Proliferation Marker; 5-Bromo-2-deoxyUridine.
储存液：0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glyce
克隆类型：Polyclonal
亚型：IgG
纯化方法：affinity purified by Protein A
**原：BrdU conjugated to KLH
交叉反应：BrdU
5-溴脱氧尿嘧啶核苷抗体细胞定位：细胞核
产品介绍：background: The immunocytochemical detection of bromodeoxyuridine (BrdU) incorporated into DNA is a powerful tool to study the cytokinetics of normal and neoplastic cells. In vitro or in vivo labeling of tumor cells with the thymidine analogue BrdU and the subsequent detection of incorporated BrdU with specific anti-BrdU monoclonal antibodies is an accurate and comprehensive method to quantitate the degree 5-溴脱氧尿嘧啶核苷抗体of DNA-synthesis. BrdU is incorporated into the newly synthezised DNA of S-phase cells may provide an estimate for the fraction of cells in S-phase. Also dynamic proliferative information such as the S-phase transit rate and the potential doubling time can be obtained, by means of bivariate BrdU/DNA flow cytometric analysis Subcellular Location: Nuclear. CAS: 59-14-3 Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. Proliferation Marker BrdU（溴化脱氧尿嘧啶核苷5-溴脱氧尿嘧啶核苷抗体）可以在体内和体外掺入到处于S期的细胞所合成的DNA链中。 此抗体可以与掺入到任何种属细胞中的BrdU反应，与碘脱氧尿 嘧啶有交叉反应，标记掺有BrdU的S期细胞，主要用于研究各种 不同的因素对正常/肿瘤组织的细胞增殖及动力学的研究。 用FITC标记的抗BrdU IgG可用于流式细胞术定量检测增值细胞。
5-溴脱氧尿嘧啶核苷抗体产品应用：ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
研究领域：细胞生物
储存条件： Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
来源： Rabbit
外观： Lyophilized or Liquid