p19ARF抑癌基因抗体
规格：1mg/1ml
英文名： CDKN2A/p19ARF
别名： MTS1; ARF; CDK4 inhibitor P16 INK4; CDKN2A; Cyclin dependent kinase inhibitor 2A; INK4; P19; P19ARF; p19INK4a.
分子量： 19kDa
储存液：0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glyce
克隆类型：Polyclonal
亚型：IgG
纯化方法：affinity purified by Protein A
**原：KLH conjugated synthetic peptide derived from human P19ARF
交叉反应：Human, Mouse, Rat,
p19ARF抑癌基因抗体细胞定位：细胞核
产品介绍：background: P19ARF Capable of inducing cell cycle arrest in G1 and G2 phases. Acts as a tumor suppressor. Binds to MDM2 and blocks its nucleocytoplasmic shuttling by sequestering it in the nucleolus. This inhibits the oncogenic action of MDM2 by blocking MDM2-induced degradation of p53 and enhancing p53-dependent transactivation and apoptosis. Also induces G2 arrest and apoptosis in a p53-independent manner by preventing the activation of cyclin B1/CDC2 complexes. Binds to BCL6 and down-regulates BCL6-induced transcriptional repression. Binds to E2F1 and MYC and blocks their transcriptional activator activity but has no effect on MYC transcriptional repression. Binds to TOP1/TOPOI and stimulates its activity. This complex binds to rRNA gene promoters and may play a role in rRNA transcription and/or maturation. Interacts with NPM1/B23 and promotes its polyubiquitination and degradation, thus inhibiting rRNA processing. Interacts with UBE2I/UBC9 and enhances sumoylation of a number of its binding partners including MDM2 and E2F1. Binds to HUWE1 and represses its ubiquitin ligase activity. May play a role in controlling cell proliferation and apoptosis during mammary gland development. Function: Capable of inducing cell cycle arrest in G1 and G2 phases. Acts as a tumor suppressor. Binds to MDM2 and blocks its nucleocytoplasmic shuttling by sequestering it in the nucleolus. This inhibits the oncogenic action of MDM2 by blockingp19ARF抑癌基因抗体 MDM2-induced degradation of p53 and enhancing p53-dependent transactivation and apoptosis. Also induces G2 arrest and apoptosis in a p53-independent manner by preventing the activation of cyclin B1/CDC2 complexes. Binds to BCL6 and down-regulates BCL6-induced transcriptional repression. Binds to E2F1 and MYC and blocks their transcriptional activator activity but has no effect on MYC transcriptional repression. Binds to TOP1/TOPOI and stimulates its activity. This complex binds to rRNA gene promoters and may play a role in rRNA transcription and/or maturation. Interacts with NPM1/B23 and promotes its polyubiquitination and degradation, thus inhibiting rRNA processing. Interacts with COMMD1 and promotes its 'Lys63'-linked polyubiquitination. Interacts with UBE2I/UBC9 and enhances sumoylation of a number of its binding partners including MDM2 and E2F1. Binds to HUWE1 and represses its ubiquitin ligase activity. May play a role in controlling cell proliferation and apoptosis during mammary gland development. Subunit: Does not interact with cyclins, CDK1, CDK2, CDK4, CDK5 or CDK6. Binds to BCL6, E2F1, HUWE1, MDM2, MYC, NPM1/B23, TOP1p19ARF抑癌基因抗体/TOPOI and UBE2I/UBC9. Interacts with TBRG1 and COMMD1. Interacts with CDKN2AIP and E4F1. Subcellular Location: Nucleus, nucleolus. Nucleus, nucleoplasm. Tissue Specificity: Widely expressed with very low levels in kidney and colon. Gene ID: 1029 Database links: Entrez Gene: 1029 Human Entrez Gene: 12578 Mouse Entrez Gene: 25163 Rat Omim: 600160 Human SwissProt: P42771 Human SwissProt: P51480 Mouse SwissProt: Q9R0Z3 Rat Unigene: 512599 Human Unigene: 4733 Mouse Unigene: 48717 Rat Entrez Gene: 1029 Human Entrez Gene: 12578 Mouse Entrez Gene: 25163 Rat Omim: 600160 Human SwissProt: P42771 Human SwissProt: P51480 Mouse SwissProt: Q9R0Z3 Rat Unigene: 512599 Human Unigene: 4733 Mouse Unigene: 48717 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. p19ARF是抑癌基因，通过干扰p53的功能，导致细胞周期调节紊乱和细胞凋亡障碍，使细胞发生癌变，与多种肿瘤的形成有关。 目前对p19ARF的研究虽然取得了很大的进展，但是还存在一些亟待解决的问题，如:p19ARF蛋白的表达调控还不是十分清楚;如何正确地认识并理解p19ARF的作用机制，p19ARF对肿瘤发生的研究有重要的理论意义;目前的许多证据大部分来源于动物实验，p19ARF与人类肿瘤的关系还需要进一步研究。
p19ARF抑癌基因抗体产品应用：WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
研究领域：肿瘤 细胞生物 信号转导 细胞凋亡 细胞周期蛋白 转录调节因子
储存条件： Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
来源： Rabbit
外观： Lyophilized or Liquid