中分子量神经丝蛋白抗体
规格：1mg/1ml
英文名： NF-M
别名： Neurofilament medium polypeptide; 160 kDa neurofilament protein; neurofilament 3; Nefm; 150kDa medium; NEF 3; NEF3; NEFM; Neurofilament 3; Neurofilament medium polypeptide; Neurofilament protein mediu
分子量： 102kDa
储存液：0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glyce
克隆类型：Polyclonal
亚型：IgG
纯化方法：affinity purified by Protein A
**原：KLH conjugated synthetic peptide derived from human NF-M
交叉反应：Human, Mouse, Rat, Pig, Cow,
中分子量神经丝蛋白抗体细胞定位：
产品介绍：background: Neurofilaments are the 10nm or intermediate filament proteins found specifically in neurons, and are composed predominantly of three major proteins called neurofilament light (NF-L), neurofilament medium (NF-M) and neurofilament heavy (NF-H). Neurofilament medium runs on SDS-PAGE gels in the range 145-170 kDa, with some variation in different species. Antibodies to this protein are useful to identify neurons and their processes in tissue sections and in tissue culture. Neurofilament medium can also be useful in studies of neurofilament accumulations seen in many neurological diseases, such as Lou Gehrig's disease or Alzheimer's disease. Function: Neurofilaments usually 中分子量神经丝蛋白抗体contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber. Post-translational modifications: There are a number of repeats of the tripeptide K-S-P, NFM is phosphorylated on a number of the serines in this motif. It is thought that phosphorylation of NFM results in the formation of interfilament cross bridges that are important in the maintenance of axonal caliber. Phosphorylation seems to play a major role in the functioning of the larger neurofilament polypeptides (NF-M and NF-H), the levels of phosphorylation being altered developmentally and coincidentally with a change in the neurofilament function. Phosphorylated in the head and rod regions by the中分子量神经丝蛋白抗体 PKC kinase PKN1, leading to the inhibition of polymerization. Similarity: Belongs to the intermediate filament family. Gene ID: 4741 Database links: Entrez Gene: 281347 Cow Entrez Gene: 4741 Human Entrez Gene: 18040 Mouse Entrez Gene: 24588 Rat Omim: 162250 Human SwissProt: O77788 Cow SwissProt: P07197 Human SwissProt: P08553 Mouse SwissProt: P12839 Rat Unigene: 458657 Human Unigene: 10971 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
中分子量神经丝蛋白抗体产品应用：WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1:10-200 IF=1:100-500 （石蜡切片需做抗原修复） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
研究领域：细胞生物 **学 神经生物学
储存条件： Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
来源： Rabbit
外观： Lyophilized or Liquid