多腺苷二磷酸多聚酶抗体/多聚ADP-核糖聚合酶1
规格：1mg/1ml
英文名： PARP1
别名： ADP ribosyltransferase (NAD+; poly (ADP ribose) polymerase); ADP ribosyltransferase NAD+; ADPRT 1; ADPRT; ADPRT1; msPARP; NAD(+) ADP ribosyltransferase 1; pADPRT 1; pADPRT1; PARP 1; PARP1; PARP-1; Pol
分子量： 112kDa
储存液：0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glyce
克隆类型：Polyclonal
亚型：IgG
纯化方法：affinity purified by Protein A
**原：KLH conjugated synthetic peptide derived from human PARP
交叉反应：Human, Mouse, Rat, Dog, Cow,
细胞定位：细胞核
多腺苷二磷酸多聚酶抗体/多聚ADP-核糖聚合酶1产品介绍：background: This gene encodes a chromatin-associated enzyme, poly(ADP-ribosyl)transferase, which modifies various nuclear proteins by poly(ADP-ribosyl)ation. The modification is dependent on DNA and is involved in the regulation of various important cellular processes such as differentiation, proliferation, and tumor transformation and also in the regulation of the molecular events involved in the recovery of cell from DNA damage. In addition, this enzyme may be the site of mutation in Fanconi anemia, and may participate in the pathophysiology of type I diabetes. [provided by RefSeq, Jul 2008]. Function: Involved in the base excision repair (BER) pathway, by catalyzing the poly(ADP-ribosyl)ation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism. This modification follows DNA damages and appears as an obligatory step in a detection/signaling pathway leading to 多腺苷二磷酸多聚酶抗体/多聚ADP-核糖聚合酶1the reparation of DNA strand breaks. Mediates the poly(ADP-ribosyl)ation of APLF and CHFR. Positively regulates the transcription of MTUS1 and negatively regulates the transcription of MTUS2/TIP150. With EEF1A1 and TXK, forms a complex that acts as a T-helper 1 (Th1) cell-specific transcription factor and binds the promoter of IFN-gamma to directly regulate its transcription, and is thus involved importantly in Th1 cytokine production. Subunit: Component of a base excision repair (BER) complex, containing at least XRCC1, PARP2, POLB and LRIG3. Homo- and heterodimer with PARP2. Interacts with PARP3, APTX and SRY. The SWAP complex consists of NPM1, NCL, PARP1 and SWAP70. Interacts with TIAM2 and ZNF423 (By similarity). Interacts (when poly-ADP-ribosylated) with CHD1L. Interacts with the DNA polymerase alpha catalytic subunit POLA1; this interaction functions as part of the control of replication fork progression. Interacts with EEF1A1, RNF4 and TXK. Subcellular Location: Nucleus.多腺苷二磷酸多聚酶抗体/多聚ADP-核糖聚合酶1 Post-translational modifications: Phosphorylated by PRKDC and TXK. Phosphorylated upon DNA damage, probably by ATM or ATR. Poly-ADP-ribosylated by PARP2. Poly-ADP-ribosylation mediates the recruitment of CHD1L to DNA damage sites. S-nitrosylated, leading to inhibit transcription regulation activity. Similarity: Contains 1 BRCT domain. Contains 1 PARP alpha-helical domain. Contains 1 PARP catalytic domain. Contains 2 PARP-type zinc fingers. Gene ID: 142 Database links: Entrez Gene: 142 Human Entrez Gene: 11545 Mouse Entrez Gene: 25591 Rat Omim: 173870 Human SwissProt: P09874 Human SwissProt: P11103 Mouse SwissProt: P27008 Rat Unigene: 177766 Human Unigene: 277779 Mouse Unigene: 11327 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PARP(poly ADP-ribose polymerase/PARP-type 1)是DNA修复酶。 PARP是细胞凋亡核心成员半胱胺酸蛋白酶(caspase)的切割底物。因此,它在DNA损伤修复与细胞凋亡中发挥着重要作用。Anti-PARP p85 是特意的PARPp85片段的特异抗体，由caspase剪切116kDa完整分子而得到的。 PARP是存在于多数真核细胞中的一个多功能蛋白质翻译后修饰酶。它通过识别结构损伤的DNA片段而被激活，对聚腺苷二磷酸核糖聚合酶PARP被认为是DNA损伤的感受器。它还能对许多核蛋白进行聚腺苷二磷酸核糖基化。因此，在DNA损伤修复与细胞凋亡中发挥着重要作用，端锚聚合酶在癌细胞端粒结构的调控机制中有重要作用。
多腺苷二磷酸多聚酶抗体/多聚ADP-核糖聚合酶1产品应用：WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
研究领域：信号转导 细胞凋亡
储存条件： Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
来源： Rabbit
外观： Lyophilized or Liquid