高迁移率族蛋白A1抗体
规格：1mg/1ml
英文名： HMGA1
别名： HMG-I; HMG-Y; High mobility group AT-hook 1; HMG-I/HMG-Y; HMG-R; HMGA 1a; HMGA 1b; HMGA1; HMGI; HMGI/Y; HMGIY; HMGY; MGC12816; MGC4242; MGC4854; HMGA1_HUMAN; High mobility group protein HMG-I/HMG-Y; H
分子量： 12kDa
储存液：0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glyce
克隆类型：Polyclonal
亚型：IgG
纯化方法：affinity purified by Protein A
**原：KLH conjugated synthetic peptide derived from human HMGA1 (5
交叉反应：Human, Mouse, Rat, Chicken, Cow,
细胞定位：细胞核
高迁移率族蛋白A1抗体产品介绍：background: HMGA1 (HMGI/Y; high mobility group AT-hook 1) is a non-histone protein involved in many cellular processes, including regulation of inducible gene transcription, integration of retroviruses into chromosomes, and the metastatic progression of cancer cells. There are two splice isoforms of HMGA1 - the full length HMGA1a/HMGI and the shortened form HMGA1b/HMGY. The encoded protein preferentially binds to the minor groove of A+T-rich regions in double-stranded DNA. It has little secondary structure in solution but assumes distinct conformations when bound to substrates such as DNA or other proteins. HMGA1 is frequently acetylated and is found in the nucleus. Interacts with HIV-1 pre-integration complex. Function: HMG-I/Y bind preferentially to the minor groove of A+T rich regions in double stranded DNA. It is suggested that these proteins could function in nucleosome phasing and in the 3'-end processing of mRNA transcripts. They are also 高迁移率族蛋白A1抗体involved in the transcription regulation of genes containing, or in close proximity to A+T-rich regions. Subunit: Interacts with HIPK2 (By similarity). Interacts with HIV-1 pre-integration complex. Subcellular Location: Nucleus. Chromosome. Post-translational modifications: Constitutively phosphorylated on two or three sites. Phosphorylated upon DNA damage, probably by ATM or ATR. Hyperphosphorylated at early stages of apoptosis, followed by dephosphorylation and methylation, which coincides with chromatin condensation. Isoforms HMG-I and HMG-Y can be phosphorylated by HIPK2. Phosphorylation of HMG-I at Ser-36, Thr-53 and Thr-78 and of HMG-Y at Thr-42 and Thr-67 by HIPK2 modulates 高迁移率族蛋白A1抗体DNA-binding affinity. HMG-Y is not methylated. Methylation at Arg-58 is mutually exclusive with methylation at Arg-60. DISEASE: Note=A chromosomal aberration involving HMGA1 is found in pulmonary chondroid hamartoma. Translocation t(6;14)(p21;q23-24) with RAD51B. Similarity: Belongs to the HMGA family. Contains 3 A.T hook DNA-binding domains. Gene ID: 3159 Database links: Entrez Gene: 3159 Human Entrez Gene: 15361 Mouse Entrez Gene: 117062 Rat Omim: 600701 Human SwissProt: P17096 Human SwissProt: P17095 Mouse SwissProt: Q8K585 Rat Unigene: 518805 Human Unigene: 4438 Mouse Unigene: 228608 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
高迁移率族蛋白A1抗体产品应用：WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
研究领域：染色质和核信号 微生物学
储存条件： Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
来源： Rabbit
外观： Lyophilized or Liquid